Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction
Paper Details
Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction
Abstract
This research was approved to isolate, detects, diagnosis and molecular composition of Escherichia coli O157 H7 from UTI in Baghdad city. 67 urine samples were collected from the referring dogs into the Adan Teaching Veterinary Hospitals from the period of October 2017 to June 2018. The urine samples were collected by using urethral catheterization method. The samples were cultured, the recovered colonies were diagnosed microscopically (Gram stain), morphorgically and biochemically according to slandered methods. The results showed that the frequency of isolated bacterial spp. were 26 (38.8%) of 67 samples of urine, sex and age disseminations of dogs with bacteriuria .The bacteria were insulated in clean culture from 20 (76.92%) samples and were insulated in diverse culture from 6 (23.07%) samples. A total of 37 strains were isolated from urine samples. Of the strains isolated, 12(32.43%) were as Escherichia coli, 7(18.91%) as Streptococcus species, 6 (16.21%) as Staphylococcus species, 4 (10.81%) as Klebsiella species. 3(8.10%) as Proteus species. and Pseudomonas aeruginosa and 2 (5.40%) as Corynebacterium species. Specific forward and reverse primers were designed according to a program from NCBI-Genbank for Escherichia coli and E. coli O157 H7 To study sequence of these genes after amplification of 16srRNA genes. Genetic identities results showed that E. coli and E. coli O157 H7 isolates gave 100% matching and resembling the reference strains.
Ball KR, Rubin JE, Chirino-Trejo M, Dowling PM. 2008. Antimicrobial resistance and prevalence of canine uropathogens at the Western College of Veterinary Medicine Veterinary Teaching Hospital, 2002-2007. Canadian Veterinary Journal 49, 985-90.
Collee JG, Fraser AG, Marmion BP, Simmons A. 1996. Mackie and McCartney practical medical Microbiology. 14th ed, Chrchill. Livingston. USA.
Etün C, Þentrk S, Levent Kocabiyik A. 2003. Bacteriological Examination of Urine Samples from Dogs with Symptoms of Urinary Tract Infection. Turkish Journal of Veterinary Animal Science 27, 1225-1229.
Greenwood D, Slack R, Peutherer J, Barer M. 2007. Medical Microbiology, 17th edition, Mosby Inc., page 233,238,305.
Griffin P, Tauxe R. 1991. The Epidemiology of Infections Caused by Escherichia coli O157:H7, Other Enter hemorrhagic E. coli, and the Associated Hemolytic Uremic Syndrome, Epidemiological Review 13, 60-98.
Hariharan H, Brathwaite-Sylvester E, Belmar VM, Sharma R. 2016. Bacterial Isolates from Urinary Tract Infection in Dogs in Grenada, and Their Antibiotic Susceptibility, Open Journal of Veterinary Medicine 6, 85- 88.
Hasan M, Yousif A, Alwan M. 2016. Detection of virulent genes in E. coli O157:H7 isolated from puppies and adult dogs by polymerase chain reaction. Research Journal for Veterinary Practitioners 4(1),1-6.
Johannes L. 2010. Shiga toxins, from cell biology to biomedical applications. National Review of Microbiology 8, 105-116.
Khakhria R, Duck D, Lior H. 1990. Extended phagetyping scheme for Escherichia coli 0157:H7, Epidemiology Infection 105, 511-520.
Kogika MM, Fortunato VAB, Mamizuka EM, Hagiwara MK, Pavan MFB, Grosso SNA. 1995. Etiologic study of urinary tract infection in dogs. Brazilian Journal of Veterinary Research Animal Science 32, 31.
Mitra M, Mahanta SK, Guha C, Pramanik AK. 1994. Comparative efficacy of gentamicin, norfloxacin and nitrofurantoin against urinary tract infection in canine. Indian Veterinary Journal 71, 1217- 1220.
Ojo O, Bello A, Amosun E, Ajadi R. 2014. Multidrug resistant verocytotoxin producing Escherichia coli O157:H7 in the faeces of diarrhoeic and non-diarrhoeic dogs in Abeokuta, Nigeria. Veterinarski Arhiv 84(1), 63-73.
Osborne CA. 1995. Three Steps to Effective Management of Bacterial Urinary Tract Infections: Diagnosis, Diagnosis, Diagnosis. Compendium 17, 1233-1240.
Quinn PJ, Carter ME, Markey B, Carter GR. 1994. Clinical Veterinary Microbiology. Wolf /Mosby, London.
Sandvig K. 2002. Pathways followed by ricin and Shiga toxin into cells. Histochemistry and Cell Biology 117(2), 131-141.
Sharma NK, Gill JPS, Joshi DV, Kwatra MS. 1992. In vitro drug susceptibility of isolates from urinary tract infection cases of canine, Indian Veterinary Medicine Journal, 16, 299-301.
Smee N, Loyd K, Grauer GF. 2013. UTIs in small animal patients: part 2: diagnosis, treatment, and complications. The Journal of the American Animal Hospital Association 49, 83-94.
Snedecor GW, Cochran WG. 1980. Statistical methods ,7th ed the Iowa state university press Ames.
Thompson MF, Litster AL, Platell JL, Trott DJ. 2011. Canine bacterial urinary tract infections: New developments in old pathogens. Veterinary microbiology 147, 205-208.
Tilley LP, Smith-Jr FWK. 2004. The 5-Minute Veterinary Consult—Canine and Feline. 3rd Edition, Lippincott Williams & Wilkins, Philadelphia, 768-769.
Trevena WB, Hooper RS, Wray C, Willshaw G.A, Cheasty T, Domingue G. 1996. Vero cytotoxin producing Escherichia coli O 157 associated with companion animals, Veterinary Record 138, 400.
Welinder-Olsson C, Kaijser B. 2005. Enter hemorrhagic Escherichia coli (EHEC), Scandinavian Journal of Infectious Diseases 37, 405-416.
Aida H. Ibrahim, Ban N.Nadhom, Ansam K. Mohammed, 2018. Detection of Escherichia coli O157 H7 isolated from infected dogs with urinary tract infections using real time – polymerase chain reaction. Int. J. Biosci., 13(4), 102-110.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.