Histopathological changes on testes of guppy fish Poecilia reticulate Peters (1859) exposed to vydate
Paper Details
Histopathological changes on testes of guppy fish Poecilia reticulate Peters (1859) exposed to vydate
Abstract
The present study was aimed to identify the histopathological changes of the testicle in the Guppy fish resulting from exposure to the vydate pesticide; the investigation conducted on 60 fish of Guppy males collected from local markets, fish were divided into 4 groups, each one included 15 fish, group 1 has exposed for (0.3 mg/L) of vydatepesticide, group 2 Exposed for (0.5mg/L), group 3 exposed for (0.8 mg/L), and total control. Then the groups were sacrificed at (2, 4 & 6) weeks and the samples of testes were collected and fixed with Bouin’s solution and processed with routine paraffin technique and tissue sections were stained with H&E stain. The results showed that the testes of the control group were surrounded by a connective tissue capsule which contained melanocytes. The testicular parenchyma composed of groups of seminiferous tubules which separated from each other by interstitial tissue which contains Lyedic cells. The germinal epithelium showed variable stages of spermatogenesis started with primordium germ cells with divided to give rise spermatogonia, primary spermatocytes, spermatid and spermatozoa. In treated groups, at two weeks exposure tovydate showed mild vascular congestion of the interstitial tissue, blood vessels and shrinkage of the Lyedic cells; while at four weeks exposure the signs revealed advanced changes within seminiferous tubules showed degenerative changes and necrosis and there were appearance of giant cells a among developing germ cells. The study concluded that the changes are increased with increasing both the concentration and the duration of exposure, as evidenced by our current study.
Al-Azawi AJM. 2010. Effect of Pesticides Gramoxone and Cypermethrin on Cyprinuscarpio and accumulation in food chain. Ph.D.thesis in Biology, College of Science, University of Baghdad, 150pp. (in Arabic).
Bagchi P, Chatterjee S, Ray A, Deb C. 1990. Effect of quinalphos, Organophosphorus insecticide, on testicular stroidegnesis in fish, Clariasbatrachus. Bulletin of Environmental Contamination and Toxicology 44, 871-875.
Bancroft JD, Stevens AS. 2012. Theory and practice of histological techniques. 7 thedition .Churchill living stone, Edinburgh, London, p. 233-250.
Barry M J, Logan D C, Ahokas JT, HoldwayDA.1995 a. Sublethal effects of endosulfan and oxygen concentration on embryos of the australian crimson – spotted rainbow fish Melanotaenia fluviatilis. Australian Journal of Ecotoxicology 1, 71- 76.
Candioti JV, Soloneski S, Larramendy ML. 2010. Genotoxic and cytotic effects of the formulated insecticide Aficida on Cnesterodon decem maculates (Jenyns, 1842) (Pisces: Poeciliidae). Mutation Research/Genetic Toxicology and Environmental Mutagenesis703, 180-186.
Choung CB, Hyne RV, Stevens MM, Hose GC. 2013. The ecological effects of a herbicide insecticide mixture on an experimental freshwater ecosystem. Environmental Pollution 172, 264-274.
Donald M, Zachary JF. 2012. Pathologic basis of veterinary disease .5 thed , Mosby. Inc. China, 60-607.
Güneş E, Yerli SV. 2011. Effects of deltamethrin on lipase activity in Guppies (Poeciliareticulata). Turkish Journal of Fisheries and Aquatic Sciences 11, 473-476.
Harvey J, Han J, Reisen R. 1978. Metabolism of oxamyl in plants. Journal of Agricultural and Food Chemistry 26, 529–536.
Kadim AH, Stevano SK, Penkow VT. 1972. Polynucleate Spermatogonial cells in the seminiferous epithelium of the field – mouse (Apodemus sylvaticus L .) . CR Akad Bulgare Science 25, 373 -376.
Li H, Mehler WT, Lydy MJ, You J. 2011. Occurrence and distribution of sediment associated insecticides in urban waterways in the Pearl River Delta, China. Chemosphere82(10), 1373-1379.
Material Safety Data Sheet. 2012. DuPont™ Vydate® L insecticide/Nematicide, Version 2.1, 13 p.
Mathews SA. 1939. The relationship between the pituitary gland and gonads in Fundulus. The Biological Bulletin 76, 241-250.
Robbins SL, Kumar V. 1987. Basic pathology. 4 thed W .B . Saunders Company.
Swarup K,Srivastava SJ. 1979. Cyclic changes in the testicular activity of the fresh water large murrel, Channa marulius. National Academy Science Letters2, 83-84.
Valavanidis A, Vlahogianni T, Dassenakis M, Scoullos M.2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety 64, 178–189.
Wasu HY, Gadhikar AY, Ade PP. 2009. Sublethal and chronic effect of Carbaryl and Malathion on Clarius batrachus(Linn.). Journal of Applied Sciences and Environmental Management13(2), 23–26.
Wolfe NL, Zepp RG, Paris DF.1978 .Use of Structure-reactivity Relationships to Estimate hydrolytic persistence of carbamate pesticides. Water Research 12(8), 561-563.
Yeh SP, Sung TG, Chang CC, Cheng W,Kuo CM. 2005. Effects of an organophosphorus insecticide, trichlorfon, on hematological parameters of the giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture 243, 383–392.
Young B, Stewart W, O’Dowd G. 2011. Wheatear’s basic pathology: A text, atlas and review of histopathology: with student consult online access, 5th ed, Churchill Livingstone, Elsevier, China, 348 p.
Nor Al-Huda R. Mayea, Mukhtar K. Haba, 2018. Histopathological changes on testes of guppy fish Poecilia reticulate Peters (1859) exposed to vydate. J. Biodiv. Environ. Sci., 12(5), 63-69.
Copyright © 2018 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.