Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba
Paper Details
Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba
Abstract
Isolation from contaminated sites using classical enrichment technique is of common practice and give promising results but with a narrow range of microbial community. In this study, wild bacteria were isolated and adopted through changing growth media and exposure to UV-light for 15minutes. Various physico-chemical conditions were optimized for pyrene degradation by isolate M3 with 1000ppm concentration, optimum temperature of 35°C, pH 7 and shaking speed of 120 rpm. Isolate M3 degraded 76.31% pyrene with 0.970 OD value and 2.7×10-31 CFUmL-1 after 120 hours. Metabolites formed during degradation process on comparison with authentic standards were identified as phenanthrene, catechol, salicylaldehyde and salicylic acid. Plasmid isolation and curing showed a fractional reduction in pyrene degradation by this isolate. Experiment was performed in triplicate and data was analysed by 2-way ANOVA and level of significance 0.002.
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Nadia Bibi, Mohammad Hamayun, Anwar Hussain, Aaqib Sayyed, Sumera Afzal Khan (2017), Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba; IJB, V10, N4, April, P343-353
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