Jasmonic acid influence on the activity of hyp-1 gene in St. John’s wort (Hypericum perforatum)
Paper Details
Jasmonic acid influence on the activity of hyp-1 gene in St. John’s wort (Hypericum perforatum)
Abstract
Considering the importance of hyp-1 gene function in hypericin biosynthesis in Hypericum perforatum, we studied the effect of various concentrations of jasmonic acid on the expression of this gene through qRT-PCR method. In this way, calli from leaf samples of St. John’s wort in vitro grown plants were treated with three concentrations of jasmonic acid (JA) and the activity of hyp-1 gene was evaluated via real-time PCR, 24, 48 and 72 hours after treatment. The results showed that in control samples there was no variation in gene expression. The samples treated with 100 μM JA, a time course increase in gene expression was observed. The same trend was seen with 500 μM JA. Accordingly, the maximum hyp-1 gene expression was witnessed in 72 hours after treatment, while the minimum was in 24 hours. In all evaluated times, increasing the concentration of JA resulted in higher expression of hyp-1 gene. Moreover, the expression of hyp-1 24 hours after increased up to about 1, 1.5 and 3 fold in the 0, 100 and 500 μM JA treatments, respectively. A similar trend was observed after 48 h, and the expression of the gene went higher up to 4 fold in 500 μM JA. After 72 h, a 5.5 fold increase was recorded in the maximum concentration of JA. It can be concluded that with increasing the concentration of JA as well as in time course, a significant increment could be seen in the expression of hyp-1 gene.
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