Occurrence of Bacillus thuringiensis from different plant areas on South Kalimantan, Indonesia
Paper Details
Occurrence of Bacillus thuringiensis from different plant areas on South Kalimantan, Indonesia
Abstract
B. thuringiensis is a gram-positive soil bacterium, and produce a crystalline inclusion body during sporulation. Therefore, many biological control of insects have been investigated. Currently, researches on the use pathogenic microorganisms to control insect pests are increasing. Microbial pest control is practiced in different parts of the world though utilization of pathogen like fungi, bacteria, viruses and nematodes.. It was a study of flacherie of the silkworm, bombxmori in this report on the discovery of sotto bacillus, which causes the disease to silkworm larvae. Five 1-g soil samples were separately suspended to 9 ml of distilled water. After allowing the suspension to stand for 5 minute, 3-4 ml of the suspension were taken. One half of the suspension was transferred to a test tube and heated in a water bath of 80oC for 15 minutes, so that all microorganisms were killed except Bacillus and other spore forming bacteria, then allowed to cool at room temperature. Ten-fold serial dilutions of the heated suspension in sterile distilled water were placed on nutrient agar (NA-pH 7.5). After two days of incubation at 28oC, Bacillus colonies were recorded. After 2 to 3 days incubation, crystalliferous spore forming bacteria were determined in phase contrast microscope. Isolation from six soil samples yielded about 50 isolates; only one was identified as B. thuringiensis. Observations on B. thuringiensis isolated from citrus areas on C. binotalis showed that infected larvae turned yellowish at the middle and hind part of the abdomen and dead larvae become shrunken and later turned black with putrid odor.
Abdel-Hameed A, Carlberg G, El-Tayeb, OM 1990. Studies on Bacillus thuringiensis H-14 strains isolated in Egypt. Screening for active strains. World Journal of Microbiology and Biotechnology. 6: 299-304.
Asano S, Bando H, Iizuka T. 1993. Amplification and identification of cryII genes from Baccilus thuringiensis by PCR procedures. Journal Sericulture. Science. Japan. 62, 223-227.
Baba F, Asano S, Iizuka T. 1990. Purification of crystals from Bacillus thurigiensis by using Percoll. Journal Sci. Jpn 59, 487-489.
Balarman K, Hoti SL, Manonmani LM. 1981. An Idigenous virulent strain of Bacillus thuringiensis, highly pathogenic and specific to mosquitoes. Current Science 50, 199-200.
Berliner E. 1915. Ober dieschalaffsucht der mehlmottenraupe (Ephestia kuhniella Zeller) und ihrenerreger Bacillus thuringiensis n. sp. Zangue. Entomol. 2, 29-56.
Bulla LA, faust RM, Andrews R, Goodman N. 1985. Insecticidal bacilli, pp. 185-209. In: the Moleculer Biology of the Bacilli, Vol. II. D. A. Dubnau (Ed.). Academic Press, New York.
Burges HD. 1981. Microbial control of pest and plant disease.1970-1980. Academic Press.949 p.
Burges HD, Hussey NW. 1971. Microbial control of insects and mites. Academic Press.876 p.
Bulla LA, Jr, Kramer KJ, Davidson LI. (1977).Characterization of the enmocidal parasporal crystal of Bacillus thuringiensis. Journal Bacteriology. 130, 375-383.
Delucca AJ, Simonson JG, Larson AD. 1981. Bacillus thuringiensis distribution in soils of the United States. Canadian Journal Microbiology. 27, 865-870.
Dulmage HT. 1992. Insecticidal activity of Bacillus thuringiensis and their potential for pest control in Microbial control for pests and plant diseases and plant diseases 1970-1980 (Ed.H.D Burges). Academic. Press. N.Y. P.
Golberg LJ, Margalit J. 1977. A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univitattus, Aedes aegypti and Culex pipiens, Mosq. New 37, 355-358.
Heimpel AM. 1967. A critical review of Bacillus thuringiensis Berl. And other crystalliferous bacteria. Annual Review Entomology 12, 287-322.
Held GA, Kawanishi CY, Huang YS. 1990. Characterization of the parasporal inclusion of Bacillus thuringiensis subsp. kyushuensis. Journal Bacteriology 481-483.
Iizuka T, Ishino M, Nakajima T. 1982. Comparative morphology of Parasporal crystal and characterization of plasmid DNA from various subspecies of entomopathogenic bacteria, Bacillus thuringiensis. Journal Faculty Agriculture. Hokkaido University. 13, 423-431.
Iizuka T, Yamamoto T. 1984. Serological properties of the mosquitocidal protein of Bacillus thuringiensis and the morphology of its parasoral crystal. Journal Faculty. Hokkaido University 62, 98-114.
Iizuka T, Sasaki J, Asano S, Bando H. 1995. Comparative studies on isolation and identification of Bacillus thuringiensis. Biotechnology and Enviroment. Benefits, I, 143-153.
Ishii T, Ohba M. 1997. Investigation of mosquito-specific larvicidal activity of a soil isolate of Bacillus thuringiensiss var. canadensis. Current. Microbiology. 35, 40-43.
Ishiwata S. 1901. On a kind of severe flacherie (sotto disease). Dainihon Sanshikaiho 114, 1-5.
Kalman S, Kiehne KK, Libs JL, Yamamoto T. 1993. Cloning of novel cryIC-type gene from a strain Bacillus thuringiensis subs. Galleriae. Applied. Enviroment. Microbiology 59, 1131-1137.
Kawalek MD, Benjamin S, Lee HL, Gill SS. 1995. Isolation and identification of novel toxin from a new mosquitocidal isolate from Malaysia, Bacillus thuringiensis subsp. Jegathesan. Applied. Enviroment. Microbiology. 2965-2969.
Kim KH, Ohba Aizawa K. 1984. Purification of the toxic protein from Bacillus thuringiensis serotype 10 isolate demonstrating a preferential larvicidal activity to mosquito. Journal Invertebrate. Pathology. 44, 214-219.
Krieg A. 1961. Bacillus thuringiensis Berliner. In disease caused by certain sporeforming bacteria. Heimpel and Angus (Eds.). 21-67.
Mattes O. 1927. Parasitarekran kheiten der mehnotten larvaen und versucheoberih reverwnd barkeitals bilogische becamp fungis mittel. (Zuheich lire beitragzurzytologie de bacgerien). Gesell f. beford, gedam, Naturw. Sitzber (Marbnog) 62, 381-417.
Ohba M, Aizawa K. 1986. Insect toxicity of Bacillus thuringiensis isolated from soils of Japan. Journal Invertebrate. Patholology. 47, 12-20.
Padua LE, Ohba M, Aizawa K. 1984. Isolation of a Bacillus thuringiensis strain (serotype 8a:8b) highly and selectively toxic against mosqouito larvae. Journal Invertebrate. Patholology. 44, 12-17.
Rejesus BM, Sayaboc A. 1990. Management of DBM with Apanteles plutellae pros fects in the Philippines. Paper presented in the second International Workshop on Diamondback Moth Management. Dec. 10-15, 1990. Tainna, Taiwan. 17 p.
Rizali A, Shin-ichiro Asano, Ken Sahara, Hisanori Bando, Bibiana W, Lay, Sugyo Hastowo, Toshihiko Iizuka. 1998. Novel Bacillus thuringiensis serovar aizawai strains isolated from mulberry leaves in Indonesia. Applied Entomolology Zoology. 33(1), 111-114.
Shorey HH, Hall IM. 1962. Effect of chemical and microbial insecticides on several insect pests of lettuce in southern California. Journal Economic Entomolology. 561, 69-174.
Yu YM, Ohba M, Gill SS. 1991. Characterization of mosquitocidal activity of Bacillus thuringiensis subsp. fukuokaensis crystal proteins. Applied. Enviroment. Microbiololgy. 1075-1081.
Zhang Y, Ku Z, Chan Z, Xu B, Yuan F, Chen G, Zhong T, Ming G. 1984. A new isolate of Bacillus thuringiensis possessing high toxicity against the mosquitoes. Acta Microbilogica Sinica 24, 320-325.
Akhmad Rizali, 2017. Occurrence of Bacillus thuringiensis from different plant areas on South Kalimantan, Indonesia. J. Biodiv. Environ. Sci., 11(6), 53-58.
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