Genotyping of rice germplasm for bacterial blight resistant gene (Xa7)

Paper Details

Research Paper 01/04/2016
Views (767)
current_issue_feature_image
publication_file

Genotyping of rice germplasm for bacterial blight resistant gene (Xa7)

Muhammad Ishaq Ibrahim, Fida Muhammad Abbasi, Malik Ashiq Rabbani, Abdul Qayyum, Sohail Ahmad Jan, Shahid Ali Khan, Haris Khurshid, Muhammad Ilyas, Ashtar Khan, Nadar Khan, Sabir Hussain Shah, Muhammad Zahid Kiani, Yasir Khan
Int. J. Biosci. 8(4), 28-35, April 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Rice (Oryza sativa) is one of most important cash crops in all over the world. Bacterial blight is a severe disease of rice caused by Xanthomonas Oryza. Abiotic control of this disease is expensive and cause environmental pollution. Therefore host plant resistance is one of the best ways to produce bacterial blight resistance in rice genotype. In present study bacterial blight resistance gene Xa7 was investigated in important rice genotypes. Bacterial blight resistance gene Xa7 was screened in 49 important rice genotypes by using specific primers linked to Xa7gene. The desire sequence (207 bp) of Xa7 gene was amplified through a set of specific sequence tag site molecular markers through Polymerase Chain Reaction (PCR). Out of 49 genotypes, 23 showed the presence of Xa-7 gene and these cultivars will be useful to control of this lethal disease.

Van der Geer J, Hanraads, JAJ, Lupton RA. 2000. The art of writing a scientific article. Journal of Scientific Communications 163, 51-59.

Abbasi FM, Masood R, Ahmed H, Khan U, Afzal M, Inamullah M, Rehman U, Khan MT, Akbar K, Abid M. 2011. Molecular screening of Pakistani rice germplasm for Xa5 gene resistance to bacterial blight. African Journal of Biotechnology 10(15), 2833-2837.

Aggarwal RK, Brar DS, Khush, GS. 1997. Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization. Molecular Genetics 254, 1-12.

Akhtar AF, Abbasi FM, Rafi A. 2005. Studies on bacterial blight of rice incidence in Pakistan. Pakistan Journal of Phytopathology 17(2), 137-140.

Akhtar MA, Zakria M, Abbasi FM. 2004. Trends in occurance of bacterial blight of rice in Pakistan. Pakistan Journal of Phytopathology 16(1), 69-71.

Amir M, Cho JI, Han M, Ahn CH, Jeon JS, An G, Park PB. 2010. The ABRE-binding bZIP transcription factor OsABF2 is a positive regulator of abiotic stress and ABA signaling in rice. Journal of Plant Physiology 167(17), 1512-1520.

Arif M, Jaffar M, Babar M, Sheikh MA, Kousar S, Arif S, Zafar Y. 2008. Identification of bacterial blight resistance genes Xa4 in Pakistani rice germplasm using PCR. African Journal of Biotechnology 7(5), 541-545.

Chen Z, Zeng HL, Yang J, Liu Q, Zhu XY. 2008. High-resolution mapping and gene prediction of Xanthomonas Oryzae pv. Oryzae resistance gene Xa7. Molecular Breeding 22, 433-441.

Chen LJ, Lee DS, Song ZP, Suh HS, Lu BR. 2004. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Anal of Botany 93, 67-73.

Dellaporta,  S.  L., Wood,  J.,  &  Hicks,  J.B. (1983). A plant mini preparation: version 2. Plant Molecular Biology and Reproduction 1, 19-31.

Huang N, Angeles ER, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush GS. 1997. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theoretical Applied Genetics 95, 313-320.

Lee KS, Rasabandith S, Angeles ER, Khush GS. 2003. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology 93, 147-152.

Lin XH, Zhang DP, Xie YF, Gao HP, Zhang Q. 1996. Identifying and mapping a new gene for bacterial blight resistance in rice based on RLFP markers. Phytopathology 86, 1156-1159.

Tan GX, Ren X, Weng QM, Shi ZY, Zhu LL, He GC. 2004. Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genetica Sinica 31, 724-729.

Ullah I, Jamil S, Iqbal MZ, Shaheen HL, Hasni SM, Jabeen S, Mehmood A, Akhter M. 2012. Detection of bacterial blight resistance genes in

Sakaguchi S. 1967. Linkage studies on the basmati rice landraces. Genetics and Molecular resistance to bacterial leaf blight, Xanthomonas Research 11(3), 1960-1966.

Mew TW, Vera CM, Cruz, Reyes RC. 1982. Interaction of xanthomonas compestris pv. Oryzae and  a  resistant  rice  cultivars.  Phytopathology 72, 786-789.

Porter BW, Chittoor JM, Yano M, Sasaki T, White  FF.  2003.  Development  and  Mapping of Markers Linked to the Rice Bacterial Blight Resistance  Gene Xa7. J. Crop Sciience 43, 1484–1492.

Sakaguchi S. 1967. Linkage studies on the resistance to bacterial leaf blight, Xanthomonas oryzae. in rice. Bulletin of the National Institute of Agricultural Sciences 16, 1-18.

Yoshimura S, Yoshimura A, Iwata N, Mcouch SR,  Abenes ML., Baraoidan MR,  Mew TW, Nelson RJ. 1995. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Molecular Breeding 1, 375-387.

Related Articles

Molecular detection and prevalence assessment of human norovirus: NoV (GI and GII) in Mugil cephalus (fish, teleost, Mugilidae) in Dakar, Senegal

Malick Ndiaye*, Malick Fall, Moussa Camara, Mamadou Malado Jallow, Ndongo Dia, Int. J. Biosci. 29(4), 144-152, October 2026.

Effects of partially replacing protein and fiber sources with cashew nut cake and cashew apple bagasse on the production performance of meat quails (Coturnix japonica Temminck & Schlegel, 1849)

N’Dri Saint-Clair Amani*, Moriféré Coulibaly, Gbohono Angelina Loukou, Howélé Ouattara, Int. J. Biosci. 29(4), 133-143, October 2026.

Mineral composition of fresh and dried peppers (Piper nigrum L.) from Côte d’Ivoire

Moussa Coulibaly*, Parfait Kouassi Kan Kouassi, Julien Koffi Golly, Doudjo Soro, Emmanuel Nogbou Assidjo, Benjamin Kouassi Yao, Int. J. Biosci. 29(4), 112-121, October 2026.

Phenotypic diversity of cocoa (Theobroma cacao L.) based on morphological characterization descriptors in major cocoa-growing areas of Tanzania

Godwin G. Maro*, Pavithravani B. Venkataramana, Kelvin M. Mtei, Mashamba Lucas Philip, Int. J. Biosci. 29(4), 102-111, October 2026.

Anti-inflammatory, analgesic and antipyretic activities of the aqueous leaf extract of Kalanchoe crenata (Andrews) Haw. traditionally used for ear disorders in Côte d’Ivoire

Monyn-Kouamé Ebalah Delphine*, Yéo Sounta Oumar, Sépé Gbodjrou Marthe, Kandé Brahima, Int. J. Biosci. 29(4), 81-89, October 2026.

General characteristics of the factors influencing the effectiveness of symbiotic relationships between legumes and bacteria

K. F. Bakhshaliyeva, N. D. İmamquliyev, M. İ. Qasımova, S. M. Muradova*, Int. J. Biosci. 29(4), 70-80, October 2026.