Investigation of GA3 effect on in vitro micro propagation of potato varieties
Paper Details
Investigation of GA3 effect on in vitro micro propagation of potato varieties
Abstract
In this investigation MS media supplemented with different concentrations of GA3 viz. 0.00, 0.12, 0.25, 0.50, 0.75mg/L were used for in vitro micro propagation of potato varieties Cardinal and Desiree. The parameters included were percent sprout, plantlet height, number of nodes per plantlet, number of days to root initiation, and number of days to transferable height of the plantlets. Statistical analysis showed that the varieties, GA3 concentration and varieties vs GA3 concentration were highly significant for plant height, number of nodes per plantlet and number of days to transferable height of the plantlets. Plantlets developed in M.S media supplemented with 0.25 mg/L of GA3 for Desiree and 0.12mg/L of GA3 for Cardinal produce maximum plant height, more number of nodes, reduced no. of days to root initiation and took less number of days to transferable height of the plant. Plantlets developed in these treatments were healthy and vigorous than the plantlets developed in other treatments. The results have shown that sprout percentage was high in variety Cardinal and Desiree proved efficient in response of no. of days to transferable height of the plant.
Adhikari DC, Ranjit M, Pant B. 2005. In vitro propagation of potato (Solanum tuberosum L.). Botanica orientalis. 5th issue, Deppt of Botany Tribuvan Universitry, Kathmandu, Nepal.
Akhtar N, Munawwar MH, Hussain M, Mahmood M. 2006. Sterile shoot production and direct regeneration from the nodal explants of potato cultivars. Asian Journal of plant Sciences 5, 885-889.
Anonymous. 2007. Agiculture Statistics of Pakistan. Ministry of Food, Agriculture and Livestock Division Economic Wing, Islamabad Pakistan.
Ben Dowling. 1995. Developing advanced seed potato technology. Australian Agribusiness Review Volum-3 No. 2.
Blom-Barnhoorn GJ, Aartrijk V. 1985. The regeneration of plants free of LSV and TBV from infected Lilium bulb-scale explants in the presence of virazola. Acta Horticulturae 164, 163-168.
Bostan H. Demirci E. 2004. Obtaing PVX, PVY and PLRV-free micro tuber from Granola, Pasinier 92 and Caspar potato cultivars. Pakistan journal of biological sciences 7(7), 1135-139.
Chaudhary B, Mitta P. 2014. The Effects of Different Concentrations and Combinations of Growth Regulators on the Micro propagation Of Potato (Solanum Tubrosum) 1(4), P-ISSN 2349-1817.
Farhatullah, Abbas Z, Abbas SJ. 2007. In vitro effects of gibberelic acid on morphogenesis of potato explant International Journal of Agriculture and Biosciences 9, 181-182.
Hussain I, Muhammad A, Chaudhry Z, Asghar R, Saqlain M, Rashid H. 2005. Morphogenic potential of three potato cultivars from divers explants, A Prerequisite in genetic manipulation. Pakistan Journal of Botany 37, 889-898.
Kaur M, Kaur R, Sharma C, Kaur N, Kaur A. 2015. Effect of growth regulators on micro propagation of potato cultivars. African Journal of Crop Science 3(5), 162-164.
Kiapour H , Moaveni P, Habibi D, Sani B. 2015. Evaluation of the application of gibbrellic acid and titanium dioxide nanoparticles under drought stress on some traits of basil (Ocimum basilicum L.) Intern-ational Journal of Agronomy and Agricultural Rese-arch 6(4) 138-150.
Liu PBW, Loy JB. 1976. Action of gibberelic acid on cell proliferation in the sub apical shoot meristem of water melon seedlings. American Journal of Botany 63, 700-704.
Madhu SV, Savithramma DL. 2014. Standar-dization of high efficient and rapid regeneration protocol for Agrobacterium mediated transformation of tomato (Solanum lycopersicum L.) cv. Pusa Ruby, Vaibhav and Arka Meghali. International Journal of Agronomy and Agricultural Research 5(5), 161-169.
Malik NJ. 1995. Potatoes in Pakistan: A Hand book. Pak-Swiss potato Development Project, PARC, Islama-bad Pakistan.
Miller PR, Amirouche L, Stuchbury T, Mathews S. 1985. The use of plant growth regulators in micro propagation of slow growing potato cultivars. Potato Research 28(4), 479-486.
Murrashige T, Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15, 473-497.
Novak FJ, Zadina, Horackova V, Maskova I. 1980. The effect of growth regulators on meristem tip development and in vitro multiplication of (Solanum tuberosum L.) plants. Potato Res 23, 155-66.
Rabbani A, Askari B, Abbasi NA, Bhatti M, Quairishi A. 2001. Effect of growth regulators on in vitro multiplication of potato International journal of agriculture & biology 3, 181-82.
Ross H. 1986. Potato breeding-problems and prosp-ectives. Journal of Plant Breeding 13, 1-132.
Siddiqui SU, Chaudhary MF, Anwar R. 1996. In vitro preservation of potato (Solanum tuberosum L.) germplasm. Pakistan Journal of Botany 28, 37-40.
Steel RGD, Torrie JH. 1980. Principles and procedure of statistics. 2nd Ed. Mc Graw Hill book company Inc. New York pp. 507.
Tisserat B, Jones D, Gallatta PD. 1992. Micro-wave sterilization of plant tissue culture media. Horticultural Science 27(4), 358-361.
Venkatasalam EP, Latawa J, Chakrabarti SK, Pandey KK, Sood R, Thakur V, Sharma AK, Singh BP. 2015. Standardisation of medium for micro propagation of recalcitrant Potato (Solanum tuberosum L.) Cultivar kufri Jyoti. Potato Journal 42(2), 116-123.
Webb KJ, Osifo EO, Henshaw GG. 1983. Shoot regeneration from leaflet discs of six cultivars of potato. Plant Science Letters 30, 1-8.
Azhar Mehmood, Abbas Hussain Shah, Azhar Hussain Shah, Muhammad Sajid, Habib Ahmad, 2016. Investigation of GA3 effect on in vitro micro propagation of potato varieties. Int. J. Agron. Agric. Res., 9(2), 190-197.
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