Investigation of GA3 effect on in-vitro micropropagation of potato varieties

Paper Details

Research Paper 01/11/2016
Views (601)
current_issue_feature_image
publication_file

Investigation of GA3 effect on in-vitro micropropagation of potato varieties

Azhar Mehmood, Abbas Hussain Shah, Muhammad Sajid, Habib Ahmad
Int. J. Agron. & Agric. Res. 9(5), 21-30, November 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

In this investigation MS media supplemented with different concentrations of GA3 viz. 0.00, 0.12, 0.25, 0.50, 0.75mg/L were used for in vitro micro propagation of potato varieties Cardinal and Desiree. The parameters included were percent sprout, plantlet height, number of nodes per plantlet, number of days to root initiation, and number of days to transferable height of the plantlets. Statistical analysis showed that the varieties, GA3 concentration and varieties vs GA3 concentration were highly significant for plant height, number of nodes per plantlet and number of days to transferable height of the plantlets. Plantlets developed in M.S media supplemented with 0.25 mg/L of GA3 for Desiree and 0.12mg/L of GA3 for Cardinal produce maximum plant height, more number of nodes, reduced no. of days to root initiation and took less number of days to transferable height of the plant. Plantlets developed in these treatments were healthy and vigorous than the plantlets developed in other treatments. The results have shown that sprout percentage was high in variety Cardinal and Desiree proved efficient in response of no. of days to transferable height of the plant.

Adhikari DC, Ranjit M, Pant B. 2005. In vitro propagation of potato  (Solanum tuberosum L.). Botanica orientalis. 5th issue, Deppt. of    Botany  Tribuvan  Universitry, Kathmandu, Nepal.

Akhtar N, Munawwar MH, Hussain M, Mahmood M. 2006. Sterile shoot production and direct regeneration from the nodal explants of potato cultivars.  Asian Journal of plant Sciences. 5:  885-889.

Anonymous.  Agiculture Statistics of Pakistan.  Ministry of Food, Agriculture and Livestock Division Economic Wing, Islamabad, Pakistan.2007.

Ben Dowling. 1995. Developing advanced seed potato technology. Australian Agribusiness Review 3(2).

Blom-Barnhoorn GJ, Aartrijk V. 1985. The regeneration of plants free of LSV and TBV from infected Lilium bulb-scale explants in the presence  of virazola. Acta Horticulturae 164, 163-168.

Bostan H. Demirci E. 2004. Obtaing PVX, PVY and PLRV-free micro tuber from  Granola, Pasinier 92 and Caspar potato cultivars. Pakistan journal of     biological sciences. 7(7),  1135-139.

Chaudhary B, Mitta P. 2014. The Effects of Different Concentrations and Combinations of  Growth Regulators on the Micro propagation Of Potato (Solanum Tubrosum). 1(4): P-ISSN 2349-1817.

Farhatullah, Abbas Z, Abbas SJ. 2007. In vitro effects of   gibberelic acid on morphogenesis of potato explant International Journal of Agriculture and Biosciences. 9, 181-182.

Hussain I, Muhammad A, Chaudhry Z, Asghar R, Saqlain M, Rashid H. 2005. Morphogenic potential of three potato cultivars from divers explants, A Prerequisite in genetic manipulation. Pakistan Journal of  Botany 37, 889-898.

Kaur M, Kaur R, Sharma C, Kaur N, Kaur A. 2015. Effect of growth regulators on micro propagation of potato cultivars. African Journal of Crop Science 3(5), 162-164.

Kiapour H , Moaveni P, Habibi D, Sani B. 2015. Evaluation of the application of gibbrellic acid and titanium dioxide nanoparticles under drought stress on some traits of basil (Ocimum basilicum L.) International Journal of Agronomy and Agricultural Research 6( 4), 138-150.

Liu PBW, Loy JB. 1976. Action of gibberelic acid on cell proliferation   in the  sub  apical shoot meristem of water melon seedlings. American Journal of Botany 63, 700-704.

Madhu SV, Savithramma DL. 2014. Standardization of high efficient and rapid regeneration protocol for Agrobacterium mediated transformation of tomato (Solanum lycopersicum L.) cv. Pusa Ruby, Vaibhav and Arka Meghali. International Journal of Agronomy and Agricultural Research.  5(5) 161-  169.

Malik NJ. 1995. Potatoes in Pakistan: A Hand book. Pak-Swiss potato Development   Project, PARC, Islamabad, Pakistan.

Miller  PR, Amirouche L, Stuchbury T, Mathews S. 1985. The use of plant growth regulators in micro propagation of slow growing potato cultivars. Potato Research 28(4), 479-486.

Murrashige T, Skoog F. 1962. A revised medium for rapid growth and  bioassay with tobacco tissue culture. Physiol. Plant. 15, 473-497.

Novak FJ, Zadina, Horackova V,  Maskova I. 1980. The effect of growth regulators on meristem tip development  and in vitro multiplication of (Solanum tuberosum L.) plants. Potato Res. 23, 155-66.

Rabbani A, Askari B,  Abbasi NA, Bhatti M, Quairishi A. 2001. Effect of growth regulators on in vitro multiplication of potato International journal of agriculture & biology 3, 181-82.

Ross H. 1986. Potato breeding-problems and prospectives. Journal of Plant Breeding. 13, 1-132.

Siddiqui SU, Chaudhary MF, Anwar R. 1996. In vitro preservation of potato (Solanum tuberosum L.) germplasm. Pakistan Journal of Botany 28, 37-40.

Steel RGD, Torrie JH. 1980. Principles and procedure of statistics. 2nd Ed. Mc Graw Hill book company Inc. New York, 507 p.

Tisserat B, Jones D, Gallatta PD. 1992. Microwave sterilization of plant tissue culture media. Horticultural Science 27(4), 358-361.

Venkatasalam EP, Latawa J, Chakrabarti SK, Pandey KK, Sood R, Thakur V, Sharma AK,  Singh BP. 2015. Standardisation of medium for     micro propagation of recalcitrant Potato (Solanum tuberosum L.) Cultivar kufri Jyoti. Potato Journal 42 (2), 116-123.

Webb KJ, Osifo EO, Henshaw GG. 1983. Shoot regeneration from   leaflet discs of six cultivars of potato. Plant Science Letters. 30, 1-8.

Related Articles

Intertidal seagrass habitat and its macroinvertebrate assemblages in Baylimango, Dapitan City

MA. Dulce C. Guillena, Int. J. Agron. & Agric. Res. 27(1), 16-26, July 2025.

Impact of moisture pit planting on growth and yield of upland Taro [Colocasia esculenta (L.) Schott]: A climate-smart strategy

J. K. Macharia, T. E. Akuja, D. M. Mushimiyimana, Int. J. Agron. & Agric. Res. 27(1), 8-15, July 2025.

Effects of three essential oils on the phytohormones production against Magnaporthe oryzae B.C. Couch, A rice blast pathogen

Ouattara Souleymane, Sama Hemayoro, Sérémé Abdoulaye, Koita Kadidia, Int. J. Agron. & Agric. Res. 27(1), 1-7, July 2025.

Boro rice cultivation practices and adaptive strategies of farmers to flash floods in Sylhet haor basin

Nurunnaher Akter, Md. Rafiqul Islam, Md. Abdul Karim, Md. Giashuddin Miah, Md. Mizanur Rahman, Int. J. Agron. & Agric. Res. 26(6), 7-18, June 2025.

Technology adoption and its impact on environmental and socioeconomic outcomes for vegetable producers in Svay Rieng Province, Cambodia

Hong Chhun, Chun Nimul, Buntong Borarin, Serey Mardy, Sao Vibol, Chan Bunyeth, Tum Saravuth, Ros Vanchey, Int. J. Agron. & Agric. Res. 26(6), 1-6, June 2025.

Effect of pigeon pea (Cajanus cajan) border crop on the control of cotton bollworms

Lovemore Mutaviri, Washington Mubvekeri, Int. J. Agron. & Agric. Res. 26(5), 122-127, May 2025.

Pruning and population density as smart solution to sustainable tomato production

Reychel I. Wamel, Artemio A. Martin Jr., Victoriano V. Casco, Lucila V. Rocha, Int. J. Agron. & Agric. Res. 26(5), 99-112, May 2025.