Genetic diversity and population structure of cavy (Cavia porcellus L) in three agro ecological zones of Côte d’Ivoire
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Genetic diversity and population structure of cavy (Cavia porcellus L) in three agro ecological zones of Côte d’Ivoire
Abstract
To investigate the genetic diversity and population structure of cavies (Cavia porcellus (Linnaeus, 1758) from three agro ecological zones (North, central, and South) of Ivory Coast, 14 microsatellites markers were used. A total of 131 cavies were genotyped. The measure of population diversity for the three populations revealed a mean allele frequency of 6.0, 5.5 and 6.429 (P<0,05) for the north, central and south populations respectively. The observed heterozygosity (Ho) was 0.511 ± 0.66, 0.505 ± 0.55 and 0.567 ± 0.064 (p < 0,05) for the north, central and south populations and in all cases lower than the expected heterozygosity (He) (0.577 ± 0.059 , 0.634 ± 0.051, 0.645 ± 0.052) respectively. This indicates low heterozygosity across the three populations in the whole population., The population specific inbreeding coefficients (FSt) were 0.1695, 0.2768 and 0.2245 (P < 0,01) for the three separate populations and a mean of 0.2257.There were no clear differences in the population structure with only 2.59 % variation among the three populations and 21.99 % variation among individuals within a population. There were high rates of inbreeding in all the three populations (mean 0.2257 (p < 0,01). Therefore the tree population would mix. It is difficult to select non-related animals and thus control inbreeding in the target populations or selection for particular traits of interest.
Botstein D, White RL, Skolnick M, Davis RW. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American. Journal of Human.Genetic 32, 314-331.
Burgos PW, Cerón-Muňoz M, Moreno-Ochoa M. 2010. Comparación de méthodos par extraccion de AND en cuyes (Cavia porcellus Rodentia, caviidae). Livestock Research and. Rural Development. 22, 4.
Chauca L. 1997. Producción de Cuyes Cavia porcellus . Food and Agriculture Organization, Rome, 80.
Evanno G, Regnaut S, Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: A simulation study. Molecular Ecology. 14, 2611-2620.
Grillenberger M, Neumann CG, Murphy SP, Bwibo NO, Weiss RE, Jiang L, Hautvast JGAJ, West CE. 2006. Intake of micronutrients high in animal-source foods is associated with better growth in rural Kenyan school children. British Journal of Nutrition, 95, 379.
Guo S, Thompson EA. 1992. Performing the exact test of hardy-Weinberg proportion for multiple alleles. Biometrics 48, 361-372.
Kanitz R, Trillmich F, Bonatto SL. 2009. Characterization of new microsatellite loci for the South-American rodents Cavia aperea and C. magna. Conservation Genetics Resources 1, 47-50.
Lammers PJ, Carlson SL, Zdorkowski GA, Honeyman MS. 2009. Reducing Food insecurity in developping countries througth meat production: The potential of the guinea pig (Cavia porcellus). Review Agriculture and Food System 24, 155-162.
Lawson HLJ, Byrne K, Santucci F, Towsend S, Taylor M, Bruford MW, Hewitt GM. 2007. Genetic structure of European sheep breeds. Heredity (2007) 99, 620-631
Metre TK. 2005. Mon compagnon: Le cobaye Cavia porcellus L., expériences personnelles au Kivu, R.D. Congo. Bulletin BEDIM 14 (1), 9-11.
Nei MT, Tateno Y. 1983. Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency. Journal of Molecular Evolution 19, 153-170.
Neumann P, Ken Tan Radloff SE, Li JJ, Hepburn HR, Yang MX, Zhang LJ. 2007. Bee-hawking by the wasp, Vespa velutina, on the honeybees Apis cerana and A. mellifera. Naturwissenschaften, 94, 469 – 472.
Pritchard J, Stephens M, Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics, 155, 945-959.
Rosenberg NA. 2004. DISTRUCT: a program for the graphical display of population structure. Molecular ecology Notes 4, 137-138.
Rossi L, Hoerz T, Thouvenot V, Pastore G, Michael M. 2006. Evaluation of health, nutrition and food security program in a complex emergency: the case of Congo as an example of a chronic post-conflict situation. Public Health Nutrition, 9(5), 551–556.
Santucci F, Ibrahim KM, Bruzzone A, Hewitt GM. 2007. Selection on MHC-linked microsatellite loci in sheep populations. Heredity (E-publ a head of print, 23 july 2007; http// dx.doi.org/10.1038/sj.hdy.6801006).
Solarte C, Cardenas H, Rosero C, Burgos W. 2007. Caracterización molecular de tres lineas comerciales de Cavia porcellus en el Departamento de Nariňo mediante marcadores moleculares AFLP. Revista Colombiana de Cienicias Pecuarias, 20, 49-58
Tapio M, Miceikiene J, Vilkki J, Kantanen J. 2003. Comparison of microsatellite and blood protein diversity in sheep: inconsistencies in fragmented breeds. Molecular Ecology, 12, 2045-2056
Weir BS, Cockerham CC. 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38, 1358-1370
Wu F, Huang Y, Ma Y, Hu S, Hao J, Li N. 2009. Evaluation of genetic diversity and relationships within and between two breeds of duck based on microsatellite markers. Progress in Natural Science, 19, 1581-1586
Parfait Kouadio Kouakou, Rob Skilton, Djikeng Apollinaire, Fantodji Agathe, Gourene Beatrice, Aoussi Serges Clément , 2015. Genetic diversity and population structure of cavy (Cavia porcellus L) in three agro ecological zones of Côte d’Ivoire. Int. J. Agron. Agric. Res., 6(3), 27-35.
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