Investigation of antimicrobial effects of tea leaf extract on microorganisms in culture media
Paper Details
Investigation of antimicrobial effects of tea leaf extract on microorganisms in culture media
Abstract
Regarding to the fact that using synthesis antimicrobial components in food stuffs has undesirable impacts including mutation, toxicological effects, and carcinogenesis in human body, they are being omitted from previously used antimicrobial components list; therefore, preparation and production of natural anti-bacterial components as a substitute seem to be necessary. Tea leaf has been recognized as a substance for antibacterial activity against the effect of microorganisms in the present research, antibacterial activity (paper-disc method), determination of the minimum inhibitor concentration and minimum microbe bactericide concentration (Agar and Broth Dilution Method) of tea water extract in three repetitions on Staphylococcus aureus (PTCC:1112), Escherichia coli (PTCC:1330) Penicillium sp. (PTCC:5251) and Clostridium perfringenes (PTCC: 1651) microorganisms were investigated. Based on the results of antimicrobial activity, Staphylococcus aureus showed the highest sensitivity and Escherichia coli had the highest resistance against tea water extract. Results of the minimum inhibitor concentration (MIC) and minimum bactericide (MBC) for Escherichia coli were determined to be 750 and 1500 mg/L and for microorganisms they were identified to be 375 and 750 mg/L respectively.
Ayaz FA, Hayırlıoglu-Ayaz S, Alpay-Karaoglu S, Grúz J, Valentová K, Ulrichová K, Strnad M. 2008. Phenolic acid contents of kale (Brassica oleraceae L. var. acephalaDC) extracts and their antioxidant and antibacterial activities. Food Chemistry 107, 19-25.
Chou CC, Lin LL, Chung KT.1999.Antimicrobial activity of tea as affected by the degree of fermentation and manufacturing season .International Journal of Food Microbiology 48, 125-130.
Imene J, Ben Slama M, Mhadhbi H, Urdaci MC, Hamdi M. 2009.Effect of green and block teas ( Camellia sinensis L.)on the charecterestic microflora of yogurt during fermentation and refrigerated storage.Food Chemistry 112,612-620 .
Ishihara N, Chu DC, Akachi S, Juneja LR.2001.Improvement of intestinal microflora balance and prevention of digestive and respiratory organ diseases in calves by green tea extracts .Livestock Production Science 68, 217-229.
Kucharia MG, Hassan MA, Ghulmanc MA . 2006.Antimicrobial effect of green tea extract on carcinogenic microorganisms isolated from high caries risk patients, a clinical study. Egyption Dental Journal 52, 2099-2106.
Kumudavally KV, Phanindrakumar HS, Tabassum A, Radhakrishna K, Bawa AS. 2008.Green tea- A potential preservative for extending(25±2). Food Chemistry 107, 426-433.
Mehrabian S. 1995. Food Microbiology, first edition, Tarbiat Moalem Publications, p. 13-32.
Mir Ahmadi F. 1999. Investigation of the effect of tea leaf extract in preventing from oils oxidation. M.Sc. Thesis, Tarbiat Modares University 49, 32-34.
Sakanaka S, JUNEJA LR, TANIGUCHI M. 2000.Antimicrobial of green tea polyphenols on thermophilic spore-forming bacteria .Journal of Bioscience and Bioengineering 90, 81-85.
Sasaki H, Matsumoto M, Tanaka T, Maeda M, Nakai M, Hamada S, Ooshima T. 2004.Antibacterial of polyphenol components in Oolong extract against Streptococcous Mutans. Caries Research 38, 2-8.
Sokmen A, Gulluce M, Akpulat HA, Daferera D,Tepe B, Polissiou M. 2004. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control 15, 627-634.
Stoicov C, Saffari R, Houghton JM. 2009.Green tea inhibits Helicobacter growth in vivo and in vitro. International Journal of Antimicrobial Agents 33, 473-478.
Tiwari RP, Bharti SK, Kaur HD, Dikshit RP, Hoondal GS.2005. Synergistic antimicrobial activity of tea &antibiotic.Indian Journal.Med.Res 122, 80-84.
Türkoğlu A, Emin MD, Mercan N. 2007. Antioxidant and antimicrobial activity of Russula delica Fr: an Edidle Wild Mushroom. Eurasian Journal of Analytical Chemistry 2, 54-67.
Weiduo Si, Joshua G, Rong T, Milosh K, Raymond Y, Yulong Y.2006. Bioassay-guided putrification and identification of antimicrobial components in Chinese green tea extract. Journal of Chromatography 1125, 204-210.
Fardin Mir Ahmadi, Kambiz Davari, 2014. Investigation of antimicrobial effects of tea leaf extract on microorganisms in culture media. J. Biodiv. Environ. Sci., 4(4), 398-403.
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