Neuro-genotoxicity assessment of sublethal exposure of carbosulfan to freshwater Fish, Cyprinus carpio (L.)
Paper Details
Neuro-genotoxicity assessment of sublethal exposure of carbosulfan to freshwater Fish, Cyprinus carpio (L.)
Abstract
Carbosulfan, a carbamate pesticide extensively employed in rural communities, enters the aquatic environment by the proximity of agricultural lands to water bodies or through direct application in such environments. The study’s goal was to investigate the neurotoxic effects of carbosulfan using ACh and AChE levels in brain tissue, and genotoxic effects using Micronucleus (MN) assay in blood cells and Comet assay in gill cells of Cyprinus carpio. The fish was exposed to 1/5th & 1/10th sublethal concentrations of 96 h LC50 for 7, 14, and 21 days. There were significant (P<0.05) alterations in ACh and AChE content and carbosulfan was induced to show MN formation and DNA damage in concentration and time-dependent manner. The reduced ionic composition in C.carpio brain tissue may explain the inhibition of AChE and the rise of ACh concentration. The significant increase in MN and DNA damage observed in carbosulfan-exposed fishes in the current study indicates the mutagenic/genotoxic potential of carbosulfan in the freshwater fish C.carpio, as well as the potential value of the Common carp for assessing pesticide pollution of freshwater bodies. Changes in these characteristics may provide an early warning signal for determining pesticide toxicity and its impact on aquatic species. As a result, it is necessary to monitor the aquatic system and forecast the hazardous effect of carbosulfan on fish; precautions should be taken while using even low concentrations of carbosulfan, and prohibiting or restricting carbosulfan usage is preferable.
Adedeji OB, Adedeji AO, Adeyemo OK, Agbede SA. 2020. Acute toxicity of diazinon to the African catfish (Clarias gariepinus). Afr. J. Biotechnol 7(5), 651-54.
Adinarayan D, Kishore S. 2018. Effect of deltamethrin in the Indian major carp Ciprinus carpio with special reference to cholinergic activities. Int. J. Recent Sci. Res 9(2), 24091-96. http://dx.doi.org/10.24327/ijrsr.2018.0902.1595
Al-Sabti K, Metcalfe CD. 1995. Fish micronuclei for assessing genotoxicity in water. Mutat. Res 343(2-3), 121-35. https://doi.org /10.1016 /0165-1218(95)90078-0
Altinok I, Capkin E, Boran H. 2012. Mutagenic, genotoxic and enzyme inhibitory effects of carbosulfan in rainbow trout Oncorhynchus mykiss, Pestic. Biochem. Phys 102(1), 61-7 https://doi.org /10.1016 /j.pestbp.2011.10.011
Anderson D, Yu TW, Philips BJ, Schmerzer P. 1994. The effects of various antioxidants and other modifying agents on oxygen radical-generated DNA damage in human lymphocytes in COMET assay. Mutat. Res. 307(1), 261-71.
APHA, AWWA, WPCP. 2005. Standard methods for the examination of water and wastewater. 21 ed. American Public Health Association, Washington, DC.
Ateeq B, Abul-Farah M, Ali MN, Ahmad W. 2002. Induction of micronuclei and erythrocyte alterations in the catfish Clarias batrachus by 2,4- dichlorophenoxyacetic acid and butachlor. Mutat. Res. 518(2), 135-44. https://doi.org/10.1016/s1383-
Augustinson KB, In: Glick D. (Ed.). 1957. Methods in Biochemical Analysis vol. 5. Interscience Publishers, New York.
Ayllon F, Garcia-Vasquez E. 2000. Induction of micronuclei and other nuclear abnormalities in European minnow Phoxinus phoxinus and mollie Poecilia latipinna: an assessment of the fish micronucleus test. Mutat. Res 467(2), 177-86. https://doi.org/10.1016/s1383-5718(00)00033-4
Belpaeme K, Delbeke K, Zhu L, Kirsch-Volders M. 1996. Cytogenetic studies of PCB77 on brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay, Mutagenesis 11(5), 485-92. https://doi.org/10.1093 /mutage/11.5.485
Burkepile DE, Moore MT, Hollandmm. 2000. Susceptibility of five nontarget organisms to aqueous diazinon exposure. Bull. Environ. Contam. Toxicol. 64(1), 114-21. https://doi.org/10.1007/ s0012899
Cadet J, Douki T, Gasparutto D, Ravanat JL. 2003, Oxidative damage to DNA: formation, measurement and biochemical features. Mutat. Res. 531(1-2), 5-23. https://doi.org/10.1016/j.mrfmmm.
Capkin E, Boran H, Altinok I. 2014.Response of Acetylcholinesterase (AChE) in the Erythrocyte and Liver of Rainbow Trout Exposed to Carbosulfan. Turkish J. Fish Aquat. Sci. 14, 643-50
Corbett JR. 1974. The Biochemical Mode of Action of Pesticides. Academic Press. 330p.
Fairbrother A, Bennett JK. 1988. The usefulness of cholin-esterase measurements. J. Wild Dis. 4, 587-90.
Finney DJ. 1971. Probit Analysis. Cambridge University Press, Cambridge 333p.
Giri S, Giri A, Sharma GD, Prasad SB. 2003. Induction of sister chromatid exchanges by cypermethrin and carbosulfan in bone marrow cells of mice in vivo. Mutagenesis 18(1), 53-8
Grisolia CK, Cordeirocm T. 2000. Variability in micronucleus induction with different mutagens applied to several species of fish. Genet. Mol. Biol. 23(1), 235-39. https://doi.org/10.1590/S1415-47572
Hulbert AJ, Pamplona R, Buffenstein R, Buttemer WA. 2007. Life and death: metabolic rate, membrane com-position, and life span of animals. Physio. Rev 87(4), 1175-213. https:// doi.org/ 10.1152 /physrev.00047.2006
International programme on chemical safety (IPCS). 1986. Carbamate Pesticide: A General Introduction. Environmental Health Criteria 64. Office of Publications, World Health Organization, Geneva, Switzerland.
Jayashree IV, Vijayalakshmi KK, Rahiman MA. 1994. The genotoxicity of Hinosan, an organophosphorous pesticide in the in vivo mouse. Mutat. Res 322(2), 77-85. https://doi.org/10.1016 /0165-1218(94)00011-5
Kaushal BT, Misha A. 2013. Investigation of acute toxicity of cadmium on snakehead fish Channa punctatus – a comparative toxicity analysis on median lethal concentration. Int. J. Adv. Biol. Res 3(2), 289-94.
Klaude M, Eriksson S, Nygren J, Ahnstrom G. 1996. The comet assay: mechanisms and technical considerations. Mutat. Res 363(2), 89-96. https:// doi.org /10.1016/0921-8777(95)00063-1
Leppert BC, Markle JC, Helt RC, Fujie GH. 1983. Determination of carbosulfan and carbofuran residues in plants, soil, and water by gas chromatography. J. Agric. Food Chem 31(2), 220-3. https://doi.org/10.1021/jf00116a009
Lowry OH, Rosebrough, NJ, Farr AL, Randall RJ. 1951. Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem. 193(1), 265-75.
Matter BE, Grauwiler J. 1974. The micronucleus test as a simple in vivo model, for the evaluation of drug induced chromosomal aberrations, Comparative studies with 13 compounds. Mutat. Res 29, 198-99.
Metcalf RL. 1951. In: Glick, D. (Ed.), Methods in Biochemical Analysis vol. 5. Interscience Publishers, New York.
Mitchelmore CL, Chipman JK. 1998. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res 399(2), 135-47. https://doi.org/10.1016 /s0027-5107(97)00252-2
Nwani CD, Lakra WS, Nagpure NS, Kumar R, Kushwaha B, Srivastava SK. 2010. Mutagenic and genotoxic effects of carbosulfan in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food Chem. Toxicol 48(1), 202-208. https://doi.org /10.1016 /j.fct.2009.09.041
Nwani CD, Nagpure NS, Kumar R, Kushwaha B, Kumar P, Lakra WS. 2011. Mutagenic and genotoxic assessment of atra- zine-based herbicide to freshwater fish Channa punctatus (Bloch) using micronucleus test and single cell gel electrophoresis. Environ. Toxicol. Pharmacol. 31(2), 314-22. http://dx.doi.org/10.1016/j.etap.2010.12.001
O’Brien RD. 1967. Insecticides. In: Action and Metabolism. Academic Press, New York.
Organization for Economic Cooperation and Development (OECD). 1992. OECD Guidelines for the Testing of Chemicals, Section 2, Test No. 203: Fish, Acute Toxicity Test. https://doi.org /10.1787
Pandey S, Nagpure NS, Kumar R, Sharma S, Srivastava SK, Verma MS. 2006. Genotoxicity evaluation of acute doses of endosulfan to freshwater teleost Channa punctatus (Bloch) by alkaline single-cell gel electrophoresis. Ecotoxicol. Environ. Saf 65(1), 56-61. https://doi.org/10.1016/ j.ecoenv.2005.
Quinn DM. 1987. Acetylcholinesterase: Enzyme structure, reaction dynamics, and virtual transition states. Chem. Rev 87, 955-79. https://doi.org /10.1021 /CR00081A005
Reddy PM, Philip GH, Bashamohideen M. 1992. Regulation of AChE system of freshwater fish, Cyprinus carpio under fenvalerate toxicity. Bull. Environ. Contam. Toxicol 48(1), 18-22. https:// doi.org /10.1007 /bf00197478
Reinecke SA, Reinecke AJ. 2004. The comet assay as biomarker of heavy metal genotoxicity in earthworms. Arch. Environ. Contam. Toxicol. 46(2), 208-15. https://doi.org/10.1007/s00244-003-2253-0
Rencüzogullari E, Topaktas M. 2000. Chromosomal aberrations in cultured human lymphocytes treated with the mixtures of carbosulfan, ethyl carbamate and ethyl methanosulfonate. Cytologia 65, 83-92.
Sao A, Pillai AK, Gupta VK. 2008. Spectrophotometric determination of carbosulfan in environmental samples. J. Sci. Ind. Res 67, 1088-91.
Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. simple technique for quantification of low levels of DNA damage in individual cell. Exp Cell Res 175(1), 184-191. https://doi.org/10.1016/0014-4827(88)90265-0
Sterhrer-Schmid P, Wolf HU. 1995. Genotoxic evaluation of three heterocyclic N-methylcarbamate pesticides using the mouse bone marrow micronucleus assay and Saccharomyces cerevisiae strain D7 and D61.M. Mutat. Res 345(3-4), 111-25. https://doi.org/10.1016/0165-1218(95)90047-0
Svensson BG, Hallberg T, Nilsson A, Schutz A, Hagmar L. 1994. Parameters of immunological competence subjects with high consumption of fish contaminated with persistent organochlorine compounds. Int. Arch. Occup. Environ. Health 65(6), 351-8. https://doi.org/10.1007/bf00383243
Topaktas M, Rencüzogullari E, Ila HB. 1996. In vivo chromosomal aberrations in bone marrow cells of rats with marshal. Mutat. Res 371(3-4), 259-64.
Van der Kloot WG. 1956. Cholinesterase and sodium transport by frog muscle. Nature 178, 366-67.
Wang Z, Zang C, Rosenfeld JA, Schones DE, Barski A, Cuddapah S, Cui K, Roh TY, Peng W, Zhang MQ, Zhao K. 2008. Combinatorial patterns of histone acetylations and methylations in the human genome. Nat. Genet 40(7), 897-903. https://doi.org/10.1038/ng.154
Yi MQ, Liu HX, Shi XY, Liang P, Gao XW. 2006. Inhibitory effects of four carbamate insecticides on acetylcholinesterase of male and female Carassius auratus in vitro. Comp. Biochem. Physiol. C. Toxicol. Pharmacol. 143(1), 1113-6.
Krishnamurthy Yogesh, Mididoddi Venkateshwarlu (2023), Neuro-genotoxicity assessment of sublethal exposure of carbosulfan to freshwater Fish, Cyprinus carpio (L.); JBES, V22, N1, January, P31-40
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