Neuro-genotoxicity assessment of sublethal exposure of carbosulfan to freshwater Fish, Cyprinus carpio (L.)

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Neuro-genotoxicity assessment of sublethal exposure of carbosulfan to freshwater Fish, Cyprinus carpio (L.)

Krishnamurthy Yogesh, Mididoddi Venkateshwarlu
J. Bio. Env. Sci.22( 1), 31-40, January 2023.
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Abstract

Carbosulfan, a carbamate pesticide extensively employed in rural communities, enters the aquatic environment by the proximity of agricultural lands to water bodies or through direct application in such environments. The study’s goal was to investigate the neurotoxic effects of carbosulfan using ACh and AChE levels in brain tissue, and genotoxic effects using Micronucleus (MN) assay in blood cells and Comet assay in gill cells of Cyprinus carpio. The fish was exposed to 1/5th & 1/10th sublethal concentrations of 96 h LC50 for 7, 14, and 21 days. There were significant (P<0.05) alterations in ACh and AChE content and carbosulfan was induced to show MN formation and DNA damage in concentration and time-dependent manner. The reduced ionic composition in C.carpio brain tissue may explain the inhibition of AChE and the rise of ACh concentration. The significant increase in MN and DNA damage observed in carbosulfan-exposed fishes in the current study indicates the mutagenic/genotoxic potential of carbosulfan in the freshwater fish C.carpio, as well as the potential value of the Common carp for assessing pesticide pollution of freshwater bodies. Changes in these characteristics may provide an early warning signal for determining pesticide toxicity and its impact on aquatic species. As a result, it is necessary to monitor the aquatic system and forecast the hazardous effect of carbosulfan on fish; precautions should be taken while using even low concentrations of carbosulfan, and prohibiting or restricting carbosulfan usage is preferable.

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