Embryonic blood vessel damage and embryotoxicity induced by electronic cigarettes vape juice
Paper Details
Embryonic blood vessel damage and embryotoxicity induced by electronic cigarettes vape juice
Abstract
This study is an investigation of electronic cigarette’s vape juice for its embryonic blood vessel damage and embryotoxicity by the use of Chorioallantoic Membrane Assay. Assessment of vascular areas showed lysis, hemorrhage, coagulation, and hyperemia in the pure grade nicotine treatment, nicotine-containing vape juice experimental treatments, and non-nicotine-containing vape juice treatments. Mean weight, mean length, percentage of dead embryos, and percentage of dysmorphogenic embryos were used as parameters to evaluate embryotoxicity. Anas platyrhynchos eggs were used as test organism. Independent Samples Kruskal-Wallis revealed that there is a significant difference in the mean weight and mean lengths of Anas platyrhynchos embryos. The percentage of dead embryos conveys possible effect of various concentrations of vape juice treatments in the death of embryos. Dysmorphogenesis in embryos was recorded in treatments containing nicotine. Results of this study can lay foundations in the areas of public awareness, consumption, education, research, and healthcare.
Ashour A, Alhussain H, Rashid U, Abughazzah L, Gupta I, Malki A, Vranic S, Moustafa AEA. 2020. E-Cigarette Liquid Provokes Significant Embryotoxicity and Inhibits Angiogenesis. Toxics 8(2), 38.
Baba S, Wikström AK, Stephansson O, Cnattingius S. 2013. Changes in Snuff and Smoking Habits in Swedish Pregnant Women and Risk for Small for Gestational Age Births. BJOG An Int. J. Obstet and Gynaecol 120(4), 456-62.
Baba S, Wikstrom AK, Stephansson O, Cnattingius S. 2014. Influence of Snuff and Smoking Habits in Early Pregnancy on Risks for Stillbirth and Early Neonatal Mortality. Nicotine Tob Res 16(1), 78-83.
Boulanger CM. 2018. Highlight on Endothelial Activation and Beyond. Arteriosclerosis, Thrombosis, and Vascular Biology 38(12).
Cardenas VM. 2019. Use of Electronic Nicotine Eelivery Systems (ENDS) By Pregnant Women I: Risk of Small-For-Gestational-Age Birth. Tob. Induc. Dis 17, 44.
Caspers KM, Romitti PA, Lin S, Olney RS, Holmes LB, Werlermm. 2013. Maternal Periconceptional Exposure to Cigarette Smoking and Congenital Limb Deficiencies. Pediatric and Perinatal Epidemiology 27(6), 509-520.
Caspers KM, Romitti PA, Lin S, Olney RS, Holmes LB, Werlermm. 2020. National Birth Defects Prevention Study. Centers for Disease Control and Prevention.
De Vito EE, Krishnan-Sarin S. 2018. Ecigarettes: Impact of E-Liquid Components and Device Characteristics on Nicotine Exposure. Current Neuropharmacology 16(4), 438-459.
Fetterman JL, Weisbrod RM, Feng B, Bastin R, Tuttle ST, Holbrook M, Baker G, Robertson RM, Conklin DJ, Bhatnagar A, Hamburg NM. 2018. Flavorings in Tobacco Products Induce Endothelial Cell Dysfunction. Arteriosclerosis, Thrombosis, and Vascular Biology 38(7), 1607-1615.
Lavezzi AM, Matturri L, Del Corno G, Johanson CE. 2013. Vulnerability Of Fourth Ventricle Choroid Plexus in Sudden Unexplained Fetal and Infant Death Syndromes Related to Smoking Mothers. Int J. Dev. Neurosci 31, 319-327.
Lee WH, Ong SG, Zhou Y, Tian L, Bae HR, Baker N, Wu JC. 2019. Modeling cardiovascular risks of e-cigarettes with human-induced pluripotent stem cell- derived endothelial cells. Journal of the American College of Cardiology 73, 2722-273.
Lerner CA, Sundar IK, Yao H, Gerloff J, Ossip DJ, McIntosh S, Robinson R, Rahman I. 2015. Vapors produced by electronic cigarettes and ejuices with flavorings induce toxicity, oxidative stress, and inflammatory response in lung epithelial cells and in mouse lung. PLoS ONE 10(2), e0116732.
Li A, Lee SY, Sirieix CM, Nattie E. 2018. Pre- and early postnatal nicotine exposure exacerbates autoresuscitation failure in serotonin-deficient rat neonates. The Journal of Physiology 596(23), 5977-5991.
Munro HM, Tarone RE, Wang TJ, Blot WJ. 2016. Menthol and Nonmenthol Cigarette Smoking. Circulation 133(19), 1861-1866.
Qasim H, Karim ZA, Rivera JO, Khasawneh FT, Alshbool FZ. 2017. Impact of Electronic Cigarettes on the Cardiovascular System. Contemporary Review.
Regan AK, Bombard JM, O’Hegartymm, Smith RA, Tong VT. 2021. Adverse Birth Outcomes Associated with Prepregnancy and Prenatal Electronic Cigarette Use. Obstetrics & Gynecology 138(1), 85-94.
Ribatti D. 2016. The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model. Mechanisms of Development 141.
Rowell TR, Reeber SL, Lee SL, Harris RA, Nethery RC, Herring AH, Glish GL, Tarran R. 2017. Flavored e-cigarette liquids reduce proliferation and viability in the CALU3 airway epithelial cell line. American Journal of Physiology-Lung Cellular and Molecular Physiology 313(1).
Schweitzer KS, Chen SX, Law S, van Demark M, Poirier C, Justice MJ, Hubbard WC, Kim ES, Lai X, Wang M, Kranz WD, Carroll CJ, Ray BD, Bittman R, Goodpaster J, Petrache I. 2015. Endothelial disruptive proinflammatory effects of nicotine and ecigarette vapor exposures. American Journal of Physiology-Lung Cellular and Molecular Physiology 309(2).
Vita JA. 2011. Endothelial Function. Circulation 124(25).
Menrie L. Arabos, Sharmilah P. Bautista, Jerome V. Domondon, Princess Faye W. Osting, Beatriz E. Aspiras, 2023. Embryonic blood vessel damage and embryotoxicity induced by electronic cigarettes vape juice. Int. J. Biosci., 23(2), 169-176.
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