A review of the benefits of using synthetic biology in molecular biology

Paper Details

Review Paper 09/08/2023
Views (578) Download (50)
current_issue_feature_image
publication_file

A review of the benefits of using synthetic biology in molecular biology

Mohammed Radhi Mohaisen
Int. J. Biomol. & Biomed.17( 1), 8-13, August 2023.
Certificate: IJBB 2023 [Generate Certificate]

Abstract

A synthetic biology system can be used for useful purposes by building artificial components. For instance, by genetically encoding a synthetic pathway in DNA, we can harness the power of enzymatic chemistry. Modern molecular biology has been enabled by synthetic biology methods, which enable the learning, testing, designing, building and repeating the cycle. In synthetic biology, cellular systems are genetically engineered from the ground up, creating new biomolecules, networks, and pathways, while rewiring and reprogramming them. A brief review of some of the most common DNA synthetic biology techniques is presented here, with a particular focus on recent advancements that have attempted to reduce the cost of synthesis and enhance DNA sequence accuracy.

VIEWS 145

Canton B, Labno A, Endy D. 2008. Refinement and standardization of synthetic biological parts and devices. Nat Biotechnol. 26(7), 787-793.

De Massy B, Dorgai L, Weisberg RA. 1989. Mutations of the phage lambda attachment site alter the directionality of resolution of holliday structures. EMBO J. 8(5), 1591-1599.

Gellert M. 1967. Formation of covalent circles of lambda dna by E. coli extracts. Proc Natl Acad Sci USA 57(1), 148-155.

Gibson DG. 2011. Enzymatic assembly of overlapping dna fragments. Methods Enzymol 498, 349-361.

Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA, Smith HO. 2009. Enzymatic assembly of dna molecules up to several hundred kilobases. Nat Methods 6(5), 343-345.

Khalil AS, Collins JJ. 2010. Synthetic biology: Applications come of age. Nat Rev Genet 11(5), 367-379.

Kikuchi Y, Nash HA. 1979. Nicking-closing activity associated with bacteriophage lambda int gene product. Proc Natl Acad Sci. USA 76(8), 3760-3764.

Kitts PA, Nash HA. 1987. Homology-dependent interactions in phage lambda site-specific recombination. Nature 329(6137), 346-348.

Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, Keasling JD. 2011. Bglbrick vectors and datasheets: A synthetic biology platform for gene expression. J. Biol. Eng. 5, 12.

Liu JK, Chen WH, Ren SX, Zhao GP, Wang J. 2014. Ibrick: A new standard for iterative assembly of biological parts with homing endonucleases. PLoS One 9(10), e110852.

Nash HA, Pollock TJ. 1983. Site-specific recombination of bacteriophage lambda. The change in topological linking number associated with exchange of dna strands. J Mol Biol 170(1), 19-38.

Nunes-Düby SE, Matsumoto L, Landy A. 1989. Half-att site substrates reveal the homology independence and minimal protein requirements for productive synapsis in lambda excisive recombination. Cell 59(1), 197-206.

Radeck J, Kraft K, Bartels J, Cikovic T, Dürr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S. 2013. The bacillus biobrick box: Generation and evaluation of essential genetic building blocks for standardized work with bacillus subtilis. J. Biol. Eng. 7(1), 29.

Røkke G, Korvald E, Pahr J, Oyås O, Lale R. 2014. Biobrick assembly standards and techniques and associated software tools. Methods Mol. Biol. 1116, 1-24.

Shetty RP, Endy D, Knight TF. 2008. Engineering biobrick vectors from biobrick parts. J. Biol. Eng. 2, 5.

Sleight SC, Bartley BA, Lieviant JA, Sauro HM. 2010. In-fusion biobrick assembly and re-engineering. Nucleic Acids Res. 38(8), 2624-2636.

Smith HO, Wilcox KW. 1970. A restriction enzyme from hemophilus influenzae. I. Purification and general properties. J. Mol. Biol. 51(2), 379-391.

Voigt C. 2011. Synthetic biology. Amsterdam: Elsevier.