Use of culture filtrates of Trichoderma strains as a biological control agent against Colletotrichum capsici causing Anthracnose fruit rot disease of chili
Paper Details
Use of culture filtrates of Trichoderma strains as a biological control agent against Colletotrichum capsici causing Anthracnose fruit rot disease of chili
Abstract
Culture filtrates of five Trichoderma strains viz. Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 were used as seed treatments aloneand in combination with culture filtrates of Colletotrichum capsici, to assay their efficacy in suppressing Anthracnose fruit rot disease and promoting chili plant growth and yield, under field conditions. A pot trial experiment was conducted at the Botanical Garden, Rajshahi University, Bangladesh from July 2006 to March 2007. Application of culture filtrates of T. harzianum IMI-392433 (T8) significantly (p=0.05) suppressed the disease percentages (94.97 %) compared to C. capsici treatment and improved both plant growth and yield. The highest seed germination rate (100%) and the highest growth and yield (20.37gm/plant) were also recorded in the same treatment; while culture filtrates of C. capsici treatment (T1) alone significantly decreased these values. The correlation matrix showed that yield of chili hadsignificant and positive correlation with plant height (r = 0.979**), number of leaf per plant (r = 0.877 **), number of primary branch (r = 0.916 **), number of secondary branch (r = 0.889**), total number of leaf (r = 0.949**) and total number of flower (r = 0.953**) at maximum flowering time, root number (r = 0.970**), root length(r = 0.923**), total number of fruit (r = 0.980**), fruit length (r = 0.935**), fresh fruit weight (r = 0.967**), dry fruit weight (r = 0.920**), total number of seed per fruit (r = 0.868**) and hundred seed weight (r = 0.955**). The significant and negative correlation (r = – 0.671**) was observed with the yield and percentages of infected fruits. The results suggest that T. harzianum IMI-392433 has growth promoting effects and this strain may be used as an effective bio control agent tocontrol Anthracnose fruit rot disease of chili.
Abdul-Baki A, Anderson JD.1973. Vigour determination of soybean seed by multiple criteria. Crop Sci 3, 630-633.
Agrios GN. 2005. Plant Pathology (5th edition). Elsevier-Academic Press, San Diego, CA, 922 pp.
Bal U, Altintas S. 2006. Effect of Trichoderma harzianum on the yield and fruit quality of tomato plants (Lycopersicum esculentum) grown in an unheated green house. Australian Journal of Experimental Agriculture 46, 131-136.
Begum MF, Rahman MA, Alam MF. 2010. Biological control of Alternaria fruit rot of chili by Trichoderma species under field conditions. Mycobiology 38(2), 113-117.
Chang YC, Baker R, Kleifield O, Chet I.1986. Increased growth of plants in the presence of the biological control agent T. harzianum. Plant Disease 70, 145-148.
Da Luz WC, Bergstrom GC, Stockwell CA. 1998. Seed applied bioprotectents for control of seed-borne Pyrenophora triticirepentis and agronomic enhancement of wheat. Canadian Journal of Plant Pathology 19, 384-386.
Dennis C, Webster J. 1971. Antagonistic properties of species-group of Trichoderma I. Production of non-volatile antibiotics. Transactions of the British Mycological Society 57, 25-39.
Elad Y. 2000. Biological control of foliar pathogens by means of Trichoderma harzianum and potential modes of action. Crop Protection 19, 709–714.
Elad Y, Freeman S. 2002. Biological control of fungal plant pathogens. In: F. Kempken, ed, A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research, The Mycota, XI. Agricultural Applications. Springer, Heidelberg, Germany, 93–109.
Elad Y, Shtienberg D. 1995. Botrytis cinerea in greenhouse vegetables; chemical, cultural, physiological and biological controls and their integration. Integrated Pest Management Review1, 15–29.
El-Katatny MH, Gudelj M, Robra KH, Elnaght M.A., Gübitz G.M. 2001. Characterization of a chitinase and an endo-β-1,3-glucanase from Trichoderma harzianum Rifaii T24 involved in control of the phytopathogen Sclerotium rolfsii. Applied Microbiology and Biotechnology 56, 137-143.
Fan O, Tian S. 2001. Postharvest biological control of grey mold and blue mold on apple by Cryptococcus albidus (Saito) Skinner. Postharvest Biology and Technology 21, 257–358.
Hanson LE. 2000. Reduction of verticillium wilt symptoms in cotton following seed treatment with Trichoderma virens. The Journal of Cotton Science 4, 224-231.
Harman GE. 2000. Myths and dogmas of biocontrol. Changes in perceptions derived from research on Trichoderma harzianum T-22. Plant Disease 84, 377-393.
Howell CR, Stipanovic RD. 1995. Mechanisms in the bio control of Rhizoctonia solani-induced cotton seedling disease by Gliocladium virens: Antibiosis. Phytopathology 85,469-472.
Intana W, Suwanno T, Chamswarng C, Chantrapromma K, Ngamriabsakul C. 2007. Increased efficacy for controlling Anthracnose of chili using antifungal metabolites from mutant strains of Trichoderma harzianum. Thai Journal of Agricultural Science 40(1-2), 65-72.
Katan T, Elad Y, Yunis H. 1989. Resistance to diethofenocarb (NPC) in benomyl resistant field isolates of Botrytis cinerea. PlantPathology 38, 86–92.
Koitabashi M. 2005. New bio control method for parsley powdery mildew by the antifungal volatiles-producing fungus Kyu-W63. Journal of General Plant Pathology 71, 280–284.
Lo CT, Lin CY. 2002. Screening strains of Trichoderma spp. for plant growth enhancement in Taiwan. Plant Pathology Bulletin 11, 215-20.
Madhosing C. 1995. Relative wilt-inducing capacity of the culture filtrates of isolates of Fusarium oxysporum f.sp. radicislycopersici, the tomato crown and root-rot pathogen. Journal of Phytopathology 4, 193-198.
Maiti D, Dasgupta B, Sen C. 1991. Antagonism of Trichoderma harzianum and Gliocladium virens isolates to Sclerotium rolfsii and biological control of stem rot of groundnut and betel vine. Journal of Biological Control 5, 105-109.
Mukhtar I. 2008. Influnces of Trichoderma species on seed germination in okra. Mycopath 6(1&2), 47-50.
Ordentlich A, Wiesman Z, Gottlieb HE, Cojocaru M, Chet I. 1992. Inhibitory furanone produced by the bio control agent Trichoderma harzianum. Phytochemistry 31, 485-486.
Ozbay N, Newman SE. 2004. Biological control with Trichoderma spp. with emphasis on T. harzianum. Pakistan Journal of Biological Sciences 7, 478-84.
Pakdeevaraporn P, Wasee S, Taylor PWJ, Mongkolporn O. 2005. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum. Plant Breeding 124(2), 206-208.
Papavizas GC. 1985. Trichoderma and Gliocladium: biology, ecology and potential for bio control. Annul Review of Phytopathology 23, 23-54.
Poldma P, Albrecht A, Merivee A. 2002. Influence of fungus Trichoderma viridi on the yield of cucumber in greenhouse conditions. In: Proceedings of the conference of scientific aspects of organic farming. Jelgava, Latvia 21-22 March 2002.
Rabeendran N, Moot DJ, Jones EE, Stewart A. 2000. Inconsistent growth promotion of cabbage and lettuce from Trichoderma isolates. New Zealand Plant Protection 53, 143-146.
Rahman MA, Begum MF, Alam MF. 2009. Screening of Trichoderma isolates as a biological control agent against Ceratocystis paradoxa causing Pineapple disease of Sugarcane. Mycobiology 37(4), 277-285.
Rahman MA. 2009. Screening of Trichoderma spp. and their efficacy as a bioconversion agent of municipal solid waste through appropriate technique of solid state fermentation. Ph.D thesis, Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh.
Ramachandran N, Madhavi Reddy K, Rathnamma K. 2007. Current status of chili Anthracnose in India. First International Symposium on Chili Anthracnose. National Horticultural Research Institute, Rural Development of Administration, Republic of Korea, September 17-19, 2007.
Shake MU. 2006. Studies on sheath blight disease of Rice (Oryza sativa L.) caused by Rhizoctonia solani and its control. M.Sc. thesis. Department of Botany, University of Rajshahi, Rajshahi, Bangladesh.
Siddaramaiah AL, Prasad KSK, Padaganar GM. 1978. Laboratory evaluation of fungicides against Cercospora moricola. (Cooke). Indian Journal of Sericulture 33, 33-36.
Sumitra R, Gaikward SJ. 1995. Cheecking Fusarium wilt of pigeon pea by Biological Means. Journal of Soils and Crops 5(2), 163-165.
Tronsmo A, Hjeljord L. 1997. Biological control with Trichoderma species. In: Boland GJ, Kuykendall LD, ed. Plant Microbe Interactions and Biological Control, Marcel Dekker, New York.
Vey A, Hoagland RE, Butt TM. 2001. Toxic metabolites of fungal bio control agents. In:Butt TM, Jacson C and Magan N, Eds. Fungi as biocontrol agents. Progress, Problems and Potential. CAB International, Bristrol,311-346.
Viterbo A, Ramot O, Chernin L, Chet I. 2002. Significance of lytic enzymes from Trichoderma spp. in the bio control of fungal plant pathogens. Antonie Van Leeuwenheek 81, 549-556.
Watanabe N. 1993. Promoting effect of Trichoderma spp. on seed germination and plant growth in vegetable. Mem Inst Sci Tech Meiji Univ 32(2), 9-18.
Wilcox WF, Harman GE, Di Pietro A. 1992. Effect of gliotoxin on growth, sporulation, and zoospore motility of seven Phytophthora spp. In vitro. Phytopathology 82, 1121.
Windham MT, Elad Y, Baker R. 1986. A mechanism for increased plant growth induced by Trichoderma spp. Phytopathology 76, 518-521.
Yoshida SS, Hiradate T, Tsukamoto K, Shirata A. 2001. Antimicrobial activity of culture filtrate of Bacallus amylolasuefaciens RC-2 isolated from mulberry leaves February. Biological Control 91, 2181– 2187.
M. Ahsanur Rahman, M. Mostafizur Rahman, Md. Kamruzzaman, Most. Ferdousi Begum, M. Firoz Alam (2012), Use of culture filtrates of Trichoderma strains as a biological control agent against Colletotrichum capsici causing Anthracnose fruit rot disease of chili; JBES, V2, N1, January, P9-18
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