Comprehensive Exploration of Phytochemical Composition and Bioactive Potentials (Antioxidant and Antibacterial Activities) in Gloriosa superba (Adavinabhi) Rhizomes

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Research Paper 04/05/2024
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Comprehensive Exploration of Phytochemical Composition and Bioactive Potentials (Antioxidant and Antibacterial Activities) in Gloriosa superba (Adavinabhi) Rhizomes

Swetha Boddu, Fouzia Begum, Elizabeth Philip, Sowkya Evangelin Pasala, Devika Tumuluri, Shouni Niveditha Tenali, Madhuri K
Int. J. Biosci.24( 5), 58-70, May 2024.
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Abstract

Gloriosa superba, commonly recognised as Adavinabhi, is a medicinal plant renowned for its traditional uses in various herbal formulations. This research aimed to explore the phytochemical substances and assess the antioxidant and antimicrobial properties of Gloriosa superba rhizomes. The phytochemical analysis involved qualitative and quantitative assessments of various bioactive compounds present in the rhizomes. Standard methods were employed to identify alkaloids, flavonoids, phenols, tannins, terpenoids, and other secondary metabolites. The quantitative estimation revealed significant concentrations of alkaloids and flavonoids, indicating their potential pharmacological relevance. Furthermore, the biological activities of Gloriosa superba rhizomes were studied. The antioxidant action was assessed using established assays such as DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging assays. Results demonstrated potent antioxidant potential, suggesting the plant’s capability to neutralize free radicals and mitigate oxidative stress. Additionally, the antimicrobial activity of Gloriosa superba rhizomes was assessed against a panel of pathogenic microorganisms, including bacteria and fungi. The plant extract exhibited significant antimicrobial effects, highlighting its potential as a natural source for antimicrobial agents. In the present study revealed that Gloriosa superba rhizomes are rich in bioactive compounds, especially alkaloids and flavonoids. The plant demonstrates notable antioxidant and antimicrobial activities, emphasizing its potential in the development of therapeutic agents. Further investigations deserve to isolate and describe specific compounds accountable for these pharmacological activities, paving the way for the development of novel pharmaceuticals and herbal remedies.

VIEWS 76

Balaji K, Kilimozhi D. 2014. GC-MS analysis of various extracts of Clerodendrum phlomidis leaf. International Journal of Pharmacy and Pharmaceutical Sciences 6(1), 226-232.

Chitra R, Rajamani K. 2009. Perise performance and correlation studies for yield and its quality characters in Glory lily Gloriosa superba (L). Academic journal of plant sciences 2, 39-43.

Gulcin I, Bursal E, Schitoglu HM, Bilsel M, Goren AC. 2010. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of Propolis from Erzurum, Turkey. Food Chem. Toxicol. 48, 2227-2238.

Ikigai H, Nakae T, Hara Y, Shimamura T. 1993.  Bactericidal catechins damage the lipid bilayer. Biochem Biophys Acta 1147, 132-6.

Janani SR, Singaravadivel K. 2014. Screening of Phytochemical and GC-MS Analysis of some bioactive constituents of Asparagus racemosus. International Journal of PharmTech Research 6(2), 428-432.

Jigna PA, Darshana JA, Sumitra CH. 2005. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology 29, 203-10.

Kala C, Farooquee N, Dhar U. 2004. Prioritization of medicinal plants on the basis of available knowledge, existing practices and use value status in Uttaranchal, India. Biodiver. Conserv. 13(2), 453-469.

Kavitha R, Kamalakannan S, Deepa T, Elamathi R, Sridhar S, Suresh kumar J. 2011. In vitro antimicrobial activity and phytochemical Analysis of Indian Medicinal plant Couroupita guianensis. Journal of Chemical and Pharmaceutical Research 3(6), 115-121.

Kumaravel S, Praveenkumar P, Vasuki P. 2010. GC-MS study on microbial degradation of Lindane. International Journal of Applied Chemistry. 6(3), 363-366.

Lavanya R, Uma Maheshwari S, Harish G, Bharath Raj J, Kamali S, Hemamalini D, Bharath Varma J, Umamaheshwara Reddy C. 2010. In vitro antioxidant activity of methanolic extract in leaves of Anisomeles malbarica Linn. Research Journal of Pharmaceutical, Biological and Chemical Sciences 1(4), 738.

Anitha. 2018. GC-MS analysis of rhizome of Gloriosa superba. Bioscience Discovery 9(3), 360-364.

Makky EA, Mashitah M, Yus off, Ibrahim MM. Impact of Medicinal plants phyto-components against Antibiotic Resistant Bacteria. Journal of Chemical and Pharmaceutical Research. 4(1), 881-893.

Muthukrishnan SD, Subramaniyan A. 2012. Phytochemical constituents of Gloriosa superba seed, tuber and leaves. Research Journal of Pharmaceutical, Biological and Chemical Sciences 3(3), 111-117.

Nadkarni KM. 1996. Indian Materia Medica, 3rd. ed, vol.2. Popular Prakashan Mumbai. 579 p.

Nikhila GS, Sangeetha G, Swapna TS. 2014. Antimicrobial screening of medicinally potent plant Gloriosa superba L. CIBTech Journal of Microbiology 3(2), 48-57.

Paul John Peter M, Yesu Raj J, Prabhu Sicis VP, Saravanan J, Sakthivel S. 2012. GC-MS Analysis of bioactive components on the Leaves extract of Stylosanthes fruticosa- A potential folklore medicinal plant. Asian Journal of Plant Science & Research 2(3), 243-253.

Priya D, Rajaram K, Sureshkumar P. 2011; Phyto chemical studies and GC-MS analysis of Caralluma fimbriata Wall. International Journal of Pharmaceutical Research and Development 3(10), 105-110.

Rehana B, Nagarajan N. 2012. Phytochemical screening for active compounds in Gloriosa superba leaves and tubers. Journal of Pharmacognosy and Phytochemistry 4, 17-20.

Senthilkumar, M. 2013. Phytochemical screening and antibacterial activity of Gloriosa superba L. Journal of Pharmacognosy and Phytochemistry 5(1), 31-36.

Shanmugham H, Rathinam R, Chinnathambi A, Venkatesan T. 2009. Antimicrobial and mutagenic properties of the root tubers of Gloriosa superba L. Pakistan Journal of Botany 41(1), 293- 299.

Sivakumar T, Gajalakshmi D, Subramanian VK, Palanisamy K. 2015. Tuber extract mediated biosynthesis of silver nanoparticles and its antioxidant, antibacterial activity. J Biol Sci; 15, 68-7.

Trease, S.E. and Evans, D. 1983. Colchicum seed and corn. In: Pharmacognosy, 12th edn. Balliere Tindall, London, 593-559 p.

Venkatachalam MR, Jebasan A. 2010. Larvicidal activity of Hydrocotyl javanica Thump extract against Culex qunquefasciatus. Journal of Experimental Zoology India 4, 99-101.