home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Isolation, identification and screening of phosphate solubilizing bacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

Paper Details

Research Paper 07/06/2024
Views (111) Download (19)
current_issue_feature_image
publication_file

Isolation, identification and screening of phosphate solubilizing bacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

M. Roja Susmitha, K. Ammani
J. Bio. Env. Sci.24( 6), 19-24, June 2024.
Certificate: JBES 2024 [Generate Certificate]
Key: Phosphate solubilizationPiper betlePseudomonas fluorescens ASN-2Rhizosphere soil

Abstract

A study was designed to screen the effective phosphate solubilizing rhizobacteria from rhizosphere of Betle vine plants (Piper betle). Soil samples collected from agriculture fields of Piper betle of Guntur district (Nutakki, Revendrapadu), Andhra Pradesh, India and they were employed for isolation of rhizobacteria by using serial dilution plate technique. To isolate rhizobacteria, two media such as Nutrient agar and Kings’ B agar were employed. Total 15 rhizobacterial strains were isolated from the rhizosphere samples and designated them as ASN-1 to ASN-15 and evaluated for their phosphate solubilizing ability. Among them, ASN-2 showed phosphate solubilisation efficiency. The maximum phosphate solubilization halo zone was recorded as 30 mm on Pikovskaya’s agar plates after 96h of incubation at 28±2⁰C whereas in case of broth, the strain exhibited highest tricalcium phosphate (TCP) solubilization as 197.32μg/ml. Characterization of the strain was performed based on cultural, morphological and genomic characteristics using 16s r RNA sequencing and identified as Pseudomonas fluorescens ASN-2 (GenBank accession number: MW537707). The study suggested that rhizobacteria (Pseudomonas) from rhizosphere of vegetable crop Piper betel of Andhra Pradesh (Nutakki, Revendrapadu) serve as a source for potential phosphate solubilizing property.

VIEWS 16
Cover PDF

Adhikari P, Jain R, Sharma A, Pandey A. 2021. Plant growth promotion at low temperature by phosphate-solubilizing Pseudomonas spp. isolated from high-altitude Himalayan soil. Microbial Ecology 82(3), 677-687.

Banerjee SR, Palit C, Sengupta D. 2010. Stress induced phosphate solubilization by Arthrobacter sp. and Bacillus sp. isolated from tomato rhizosphere. Australian Journal of Crop Science 4, 378-383.

Bhatt PV, Vyas BRM. 2014. Screening and characterization of plant growth and health promoting rhizobacteria. International Journal of Current Microbiology and Applied Sciences 3(6), 139-155.

Bhattacharyya PN, Jha DK. 2012. Plant growth-promoting rhizobacteria emergence in agriculture. World Journal of Microbiology and Biotechnology 28, 1327-1350.

Bychkova AA, Zaitseva YV, Sidorov AV, Aleksandrova AS, Marakaev OA. 2022. Biotechnological potential of phosphate-solubilizing Pseudomonas migulae strain GEOT18. International Journal of Agricultural Technology 18(4), 1403-1414.

Cappuccino JC, Sherman N. In Microbiology-a laboratory manual 6, 491-496.

Chung H, Park M, Madhaiyan M. 2005. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere crop plants of Korea. Soil Biology and Biochemistry 37, 1970-1974.

Dave A, Patel HH. 1999. Inorganic phosphate solubilizing soil Pseudomonads. Indian Journal of Microbiology 39, 161-164.

Espinosa VD, Lopez RL, Cruz BAU. 2009. Use of 16s RNA gene for characterization of phosphate-solubilizing bacteria associated with corn. Revista Fitotecnia Mexicana 32, 31-37.

Grant MA, Holt JG. 1977. Medium for the selective isolation of members of the genus Pseudomonas from natural habitats. Applied and Environmental Microbiology 33(5), 1222-1224.

Gulati A, Rahi P, Vyas P. 2008. Characterization of phosphate-solubilizing fluorescent Pseudomonas for the rhizosphere of sea-buckthorn growing in the cold desert of Himalayas. Current Microbiology 56, 73-79.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. 1994. Bergys Manual of Determinative Bacteriology, USA 9, 112-115.

Inchure SM, Vedpathak DV. 2021. Studies on phosphate solubilizing potential of marine Pseudomonas isolates. Journal of Advanced Scientific Research 12.

Khan AA, Jilani G, Akhtar MS. 2009. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. Journal of Agricultural and Biological Sciences 1, 48-58.

King EO, Ward MK, Raney DE. 1954. Two simple media for demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44, 301-307.

Kshetri L, Nevita T, Pandey P. 2015. Plant growth promoting rhizobacteria (PGPR) and their application for sustainable agriculture in north eastern region of India. Environmental Bulletin of Himalayan Ecology 23, 1-47.

Mahantesh P, Patil CS. 2011. Isolation and biochemical characterization of phosphate solubilizing microbes. International Journal of Microbiological Research 3(1), 67.

Paul D, Sinha SN. 2017. Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India. Annals of Agricultural Sciences 15(1), 130-136.

Pikovskaya RI. 1948. Mobilization of phosphorous in soil in connection with vital activity of some microbial species. Mikrobiologiya 17, 362-370.

Rodriguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17, 319-329.

Sujatha N, Ammani K. 2014. Phosphate solubilization by the isolates of fluorescent Pseudomonads. International Journal of Advanced Pharmaceutical and Biological Chemistry 3.

Tallapragada P, Seshachala U. 2012. Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betle in Karnataka, India. Turkish Journal of Biology 36(1), 25-35.

Watanabe FS, Olsen SR. 1965. Test of an ascorbic acid method for determining phosphorous in water and NaHCO3 extracts from soil. Soil Science Society of America Journal 29, 677-678.

Submit Manuscript