Isolation and identification with different enzyme production from marine associated plants

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Research Paper 04/09/2024
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Isolation and identification with different enzyme production from marine associated plants

J. Valli, K. Kanimozhi, V. Ambikapathy, A. Panneerselvam
Int. J. Biosci.25( 3), 12-20, September 2024.
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Abstract

The current research focuses on the diversity and screening of endophytic fungi found within three distinct marine-associated plants, which reside inside the host plant without causing any observable negative impact on them like Avicennia marina, Suaeda maritima, Salicornia brachiata. The maximum number of colonies was isolated from Avicennia marina, and Salicornia brachiata followed by Suaeda maritima. The eighteen fungi were identified such  as Aspergillus conicus, A. fumigatus, A. niger, A. luchuensis, A. ochraceus, A. terreus, A. ustus, Alternaria geophylla, Alternaria tenuis, Choanephora cucurbitarum, Curvularia geniculata, Fusarium falcatum, Helminthosporium sativum, Neonectria ranularia, Nigrospora sphaerica, Penicillium janthenellum, Pyricularia oryzae, Rhizopus stolonifer by  morphological  characters were significantly resulted. The fungal strains were screened by amylase, cellulase, lipase, and protease production. Among them only fungal strain were maximum (A. niger) and minimum zone of inhibition (Choanephora cucurbitarum) were observed in protease production followed by amylase. Maximum produced cellulase (A. ochraceus) and minimum zone of inhibition (Rhizopus stolonifer) followed by lipase. Totally these (Aspergillus conicus, A. ochraceus) two fungi are present in all enzyme production. However, these endophytic fungi were excellent biological activities for future endeavor.

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