Anticancer activity of Biophytum sensitivum ethanolic extract against cervical carcinoma cell lines
Paper Details
Anticancer activity of Biophytum sensitivum ethanolic extract against cervical carcinoma cell lines
Abstract
Cancer is a debilitating disease resulting from uncontrolled proliferation. One major treatment strategy for cancer is the application of chemotherapeutic drugs which kill cancer cells. In this study the anticancer potentials of plants was investigated against HeLa and HT-3 cell line. Cytotoxicity of leaves extracts was determined by MTT assay. The results showed that the ethanolic extract of Biophytum sensitivum possessed a moderate amount of anticancer activity and the IC50 value was recorded. The most potent anticancer activity was observed with the ethanolic extract of Biophytum sensitivum with IC50 values of 61.25µg/ml and 56.25µg/ml on HeLa and HT-3 cells respectively. Phytochemical analyses revealed the presence of large amount of phenols and flavonoids in the potent plant extracts which may be suggested to play an important role in their anticancer activities. The ethanolic extract would be studied further for isolation and characterization of active components for lead optimization studies.
Abdullaev FI. 2001. Plant derived agents against cancer. In: Gupta SK (ed.), Pharmacology and therapeutics in the new millennium, Narosa Publishing House: New Delhi, India, 345–354.
Abinash C, Bharati, Sahu AN. 2012. Ethnobotany, phytochemistry and pharmacology of Biophytum sensitivum DC. Pharmacognosy Reviews 6(11), 68–73.
Bhaskar VK, Rajalakshmi V. 2010. Antitumour activity of aqueous extract of Biophytum sensitivum Linn. Annals of Biological Research 3, 76–80.
Boominathan M, Ramamurthy V. 2009. Antimicrobial activity of Heliotropium indicum and Coldenia procumbens. Journal of Ecobiology 24(1), 11–15.
Cragg GM, Newman DJ. 2005. Plants as source of anticancer agents. Journal of Ethnopharmacology 100, 72–79.
Di Domenico F, De Marco F, Perluigi M. 2013. Proteomics strategies to analyze HPV-transformed cells: relevance to cervical cancer. Expert Review of Proteomics 10(5), 461–472.
Dredge K, Dalgleish AG, Marriott JB. 2003. Angiogenesis inhibitors in cancer therapy. Current Opinion in Investigational Drugs 4, 667–674.
Evans WC. 2002. Trease and Evans Pharmacognosy, 15th Ed. W.B. Saunders, London, 183–184 and 191–393.
Ferlay J, Soerjomataram I, Dikshit R. 2015. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. International Journal of Cancer 136(5), E359–E386.
Harborne JB. 1973. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman and Hall, London, 33–41.
Haslem E. 1989. Plant polyphenols: Vegetable tannins revisited – chemistry and pharmacology of natural products. Cambridge University Press, Cambridge, 169.
Jayaprakasam B, Zhang Y, Seeram NP, Nair MG. 2003. Growth inhibition of human tumor cell lines by withanolides from Withania somnifera leaves. Life Sciences 74, 125–132.
Jayvir A, Minoo P, Gauri B, Ripal K. 2002. Nature Heals: A Glossary of Selected Indigenous Medicinal Plant of India, 2nd Ed. SRIST Innovations, India, 22.
Jirovetz L, Wobus A, Buchbauer G, Shafi MP, Thampi PT. 2004. Comparative analysis of the essential oil and SPME-headspace aroma compounds of Cyperus rotundus L. roots/tubers from South-India using GC, GC-MS and olfactometry. Journal of Essential Oil Bearing Plants 7, 100–106.
Kontostathi G, Zoidakis J, Anagnou NP, Pappa KI, Vlahou A, Makridakis M. 2016. Proteomics approaches in cervical cancer: focus on the discovery of biomarkers for diagnosis and drug treatment monitoring. Expert Review of Proteomics 13(8), 731–745.
Kunle OF, Egharevba HO. 2009. Preliminary studies on Vernonia ambigua: phytochemistry and antimicrobial screening of whole plant. Ethnobotanical Leaflets 13, 1216–1221.
Mossman T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65, 55–63.
Ochwangi DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. 2014. Medicinal plants used in treatment and management of cancer in Kakamega County, Kenya. Journal of Ethnopharmacology 151(3), 1040–1055.
Oloyode OI. 2005. Chemical profile of unripe pulp of Carica papaya. Pakistan Journal of Nutrition 4(6), 379–381.
Pappa KI, Polyzos A, Jacob-Hirsch J. 2015. Profiling of discrete gynecological cancers reveals novel transcriptional modules and common features shared by other cancer types and embryonic stem cells. PLoS ONE 10(11), e0142229.
Rao BR, Singh K, Sastry KP, Singh CP, Kothari SK, Rajput DK, Bhattacharya AK. 2007. Cultivation technology for economically important medicinal plants. In: Janardhan Reddy K, Bahadur B, Bhadraiah B, Rao MLN (eds), Advances in Medicinal Plants. Universities Press India Pvt Ltd, Hyderabad, 112–122.
Schiffman MH, Castle P. 2003. Epidemiologic studies of a necessary causal risk factor: human papillomavirus infection and cervical neoplasia. Journal of the National Cancer Institute 95(6), article E2.
Shivanna MB, Mangala KR, Parinitha M. 2008. Ethno-medicinal knowledge of Lambani community in Chikamagalur district of Karnataka. Indian Journal of Medicinal and Aromatic Plant Sciences 30, 105–108.
Sivaraj R, Rahman PKSM, Rajiv P, Salam HA, Venckatesh R. 2014. Biogenic copper oxide nanoparticles synthesis using Tabernaemontana divaricata leaf extract and its antibacterial activity against urinary tract pathogen. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 133, 178–181.
Sofowora EA. 1993. Medicinal Plants and Traditional Medicine in Africa. John Wiley and Sons Ltd, Nigeria, 1–3.
Tian J, Wong KKY, Ho CM, Lok CN, Yu WY, Che CM, Chiu JF, Tam PKH. 2007. Topical delivery of silver nanoparticles promotes wound healing. Nanomedicine 2(1), 129–136.
Trease GE, Evans WC. 1983. Textbook of Pharmacognosy, 12th ed. Balliere Tindall, London, 57–59.
Varghese AC, Ly K, Corbin C, Mendiola J, Agarwal A. 2010. Oocyte development competence and embryo development: Impact of lifestyle and environment and risk factors. RBM Online 22(5), 410–420.
H. P. Reni Christabel, T. S. Dhanaraj, V. Ramamurthy (2025), Anticancer activity of Biophytum sensitivum ethanolic extract against cervical carcinoma cell lines; IJB, V26, N5, May, P134-141
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