Paper Details

Effect of genotype and various hormonal combinations on regeneration of sunflower (Helianthus annuus L.)

Jamil Nokhasi,  Afsaneh Khosravi, Amir Fayaz Moghadam

DOI: https://dx.doi.org/10.12692/ijb/5.10.119-127

Int. J. Biosci. 5(10), 119-127. November, 2014. (PDF)

Abstract:

In this study, the effect of cotyledon and hypocotyl explants of five sunflower genotypes (Azargol, Farokh, Maste, CIRENA  and ESBIBA) were studied  on different  hormonal  combinations  IAA, NAA, BAP, KIN and 2,4-D  on regeneration of Sunflower. Variance analysis results showed that evaluated cultivars had significant differences in response to different hormonal combinations.   The results of the mean comparison showed that maximum amount of callus was obtained in hypocotyl explant, Farokh genotype in all media. Maximum number of airbud was observed in cotyledon explants of Master and ESBIBA genotypes in medium which was containing hormonal combination (IAA+BAP)   as well as, ESBIBA genotype in medium which was containing hormonal combination (IAA+KIN). Also maximum  amount  of  rooting  were  related to  hypocotyl explants of  Azargol  and  ESBIBA genotypes, in media which were containing hormonal combination (NAA+KIN) as well as CIRENA genotype in medium which was containing hormonal combination (NAA+BAP).