Paper Details

Development of PCR assay for detection of Toxoplasma gondii in meat products in south west of Iran

Rahmatollah Yazdani, Abbas Doosti, Marzieh Shavali, Asghar Arshi

DOI: https://dx.doi.org/10.12692/ijb/5.10.128-133

Int. J. Biosci. 5(10), 128-133. November, 2014. (PDF)

Abstract:

Toxoplasma gondii (T. gondii) is an intracellular protozoan parasite with felids as definitive hosts, including humans and domestic animals and is an important cause of abortions and stillbirths. The aim of present study was to identification of T. gondii in meat products by PCR assay in Chaharmahal Va Bakhtiari province (south west of Iran). In the present study, 273 meat products were collected from different companies and food markets. Genomic DNA was extracted and PCR was performed for B1 gene amplification of meat products using specific oligonucleotid primers (Tg1 and Tg2). Results showed that 30 out of 273 samples (10.98 %) were positive for T. gondii. These findings showed relatively high incidence of T. gondii infection in meat products in south west of Iran.