Paper Details

In vitro pollen viability and pollen germination of service tree (Sorbus domestica L.)

Melekber Sulusoglu, Aysun Cavusoglu

DOI: https://dx.doi.org/10.12692/ijb/5.8.108-114

Int. J. Biosci. 5(8), 108-114. October, 2014. (PDF)

Abstract:

We investigated the pollen viability and germination of naturally grown service tree (Sorbus domestica L.). This is the first study on the pollen germination of service tree. Two staining tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (Iodine potassium iodide) were used. There were clear differences among the staining tests and IKI gave the highest viability percentages. In the initial studies, different sucrose concentrations (0, 5, 10, 15 or 20%) were added into the media and 15% sucrose gave the highest pollen germination. In the next study, 0, 25, 50, 75 or 100 mg/l boric acid added into media together with 1% agar and 15% sucrose to determine the effects of boric acid concentrations. The highest pollen germination has occurred in boric acid-free media. Pollen germination was inhibited in the higher boric acid concentrations and pollen tube development has continued until 75 mg/l concentration.