Paper Details

In vitro phytochemical, cytoxicity and mineral composition analyses of Micania cordata (Bumr.f.) B. L. robinson leaves

Nobel Barua, Nurul Absar, Sudip Paul, Anik Barua, Md. Yeashin Gazi, Moumoni Saha, Mohammad Shariful Islam, Jannatul Marium Belaly

DOI: https://dx.doi.org/10.12692/ijb/5.8.154-160

Int. J. Biosci. 5(8), 154-160. October, 2014. (PDF)

Abstract:

Mikania cordata (Family: Asteraceae) has been used in traditional medication for the ailments of human diseases. The present study was designed to explore the phytochemical constituents and cytotoxic activity of ethanolic extract of M. cordata (Bumr.f.) B.L. Robinson leaves. The preliminary phytochemical screening revealed the presence of reducing sugars, flavonoids, saponins, alkaloids, tannins, phlobotannins, steroids, cardiac glycosides, amino acids in the ethanolic extract of the plant leaves. The ethanolic extract did not show any noticeable toxicity in brine shrimp lethality bioassay. The LC50 and LC90 values were found to be 102.09 and 971.63 μg/ml respectively for the plant extract. The mineral composition of the plant leaves was also analyzed in the present study by atomic absorption spectrophotometer. The mean (±SD) content of calcium, potassium, magnesium, phosphorus, sulpher, iron, manganese and zinc was 306.00±3.60, 116.67±1.53, 54.67±1.15, 0.09±0.005, 0.58±0.02, 85.67±1.53, 5.67±0.60 and 36.00±1.73 (in μg/g) respectively in the leaves of M. cordata. Thus the study reveals that leave’s extract contains several phytochemicals, show less cytotoxicity and the leaves also contain considerable amount of minerals. The findings of the study provide the basis for its wide use as the therapeutic both in traditional and folk medicine.