Paper Details

Preservation of spotted buffalo epididymal spermatozoa in andromed with raffinose addition

Muhammad Rizal, Yulnawati Yusnizar, Hera Maheshwari, Herdis

DOI: https://dx.doi.org/10.12692/ijb/5.8.244-249

Int. J. Biosci. 5(8), 244-249. October, 2014. (PDF)

Abstract:

Raffinose is an external cryoprotectant that would protect cell membrane from cold shock effect during preservation process. This study was perform to observe the effect of raffinose on the quality of spotted buffalo epididymal spermatozoa during storage at 4 °C for 24 h. Three different extenders, i.e. commercial tris-egg yolk free extender (Andromed) as control (AM), Andromed Andromed plus 0.2% w/v raffinose (AMR0.2), and Andromed plus 0.4% w/v raffinose (AMR0.4), were used in this study. The results showed that the progressive motility and viability of spermatozoa reduced significantly during storage period. At 12 h of storage, the progressive motility was 60.33% in AM versus 60% in both AMR0.2 and AMR0.4 (P>0.05). Meanwhile, after 24 h of storage, the progressive motility in both AM and AMR0.4 extenders was 55% versus 55.33% in AMR0.2 (P>0.05). Spermatozoa viability in AM, AMR0.2, and AMR0.4 were 70.30%, 73%, 72.33% after 12 h of storage and 66.33%, 68.67% and 69.67% after 24 h of storage, respectively (P>0.05). This results suggested that Andromed without an external cryoprotectant addition could maintain the quality of epididymal spermatozoa as good as the one with 0.2% and 0.4% raffinose addition.