Paper Details

In vitro regeneration of two chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars through organogenesis from petal explants

Rezvanolsadat Kazeroonian, Amir Mousavi, Sepideh Kalatejari, Masoud Tohidfar

DOI: http://dx.doi.org/10.12692/ijb/5.4.76-81

Int. J. Biosci. 5(4), 76-81. August, 2014. (PDF)

Abstract:

Petal explants of two chrysanthemum cultivars were cultured on the Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators (PGRs). Results indicated the supremacy of “Resomee Splendid” over the other cultivar in regard to the shoot induction percentage with 62.22% vs. 49.63%. Maximum percentages of the shoot induction (93.33% vs. 73.33) were achieved in 3.0 mg l-1 benzylaminopurine (BAP) + 0.5 mg l-1 1-naphthaleneacetic acid (NAA) and 4.5 mg l-1 BAP +1.0 mg l-1 NAA, while, the highest number of shoots per explant (2.73 vs. 3.0) was obtained in 4.5 mg l-1 BAP + 1.0 mg l-1 NAA in “Resomee Splendid” and “Reagan Elite Salmon”, respectively. Considering callogenesis, the later cultivar totally showed a greater response to the PGR treatments. Adventitious shoots were formed indirectly on the “Resomee Splendid” explants, while, considering the other cultivar direct shoot formation was observed on the media fortified with either 3.0 mg l-1 BAP + 0.5 mg l-1 NAA or 4.5 mg l-1 BAP + 0.5 mg l-1 NAA. A combination of thidiazuron (TDZ) and NAA resulted in indirect shoot formation of both cultivars. Regenerated shoots successfully elongated and formed roots on MS medium and were finally acclimatized and transplanted into soil. Treatments containing TDZ were totally inferior to the ones comprising of BAP in terms of both quantitative characteristics.