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Partial purification and some properties of Vibrio haemolyticus protease

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Research Paper 01/03/2013
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Partial purification and some properties of Vibrio haemolyticus protease

T. O. Femi-Ola, O. S. Bamidele
Int. J. Biosci.3( 3), 92-97, March 2013.
Certificate: IJB 2013 [Generate Certificate]
Key: CaseinInhibitorsKolanutProteaseVibrioWeevil

Abstract

An extracellular protease of Vibro haemolyticus isolated from the gut of kolanut weevil Balanogastris kolae Desbr was partially purified and characterized in this study. The enzyme was purified in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-150 gel filtration chromatography. The purified protease had an apparent molecular weight of 22.3kDa. The protease was found to have optimum activities at temperature of 50oC and pH of 8. The maximum velocity Vmax and Km of the protease produced during the hydrolysis of casein were 66.66mg/min/mL and 3.33mg/mL. The enzyme was almost 100% stable at 60oC even after 120 minutes of incubation. It was strongly activated by K+, while the enzyme activity was strongly inhibited by Fe3+ and Hg2+. .

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Aderibigbe EY. 1998. Characterization of extracellular proteinases from strains of from strains of Bacillus group. African Journal Science 2, 100-107.

Adinarayana K, Ellaiah P, Prasad DS. 2003. Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11. AAPS Pharmaceutical Science Technician 4, 56-63.

Beg QK, Sahai, V, Gupta R. 2003. Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor. Process Biochemistry 39, 203-209, http://dx.doi.org/10.1016/s0032-9592(03)00064-5

Fujiwara N, Yamamoto K. 2007. Production of alkaline protease in low cost medium by alkalophilicBacillus species and properties of the enzyme. Journal of Fermentation Technology 65, 345-348.

Gupta R, Beg QK, Lorenz P. 2002. Bacterial alkaline protease: Molecular approaches and industrial applications. Applied Microbiology and Biotechnology 59, 15-32m http://dx.doi.org/10.1007/s00253-002-0975-y

Jaswal RK, Kocher GS. 2007. Partial characterization of a crude alkaline protease from Bacillus circulans and its detergent compatibility. The Internet Journal of Microbiology 4, 1.

Kim JM, Lim WJ, Suh HJ. 2001. Feather degrading Bacillus species from poultry waste. Process Biochemistry 37, 287-291, http://dx.doi.org/10.1016/S0032-9592(01)00206-0

Lee CY, Chang MF, Yu MS, Pan MJ. 2002. Purification and characterization of a putative virulence factor, serine protease from Vibrio parahaemolyticus FEMS Microbiology Letter 19, 31-37, http://dx.doi.org/10.1111/j.1574-6968.2002tb10982

Lowry QH, Rosebrough NJ, Farr AL, Randall RL. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265-273.

Madan M, Dhillion S, Singh R. 2002. Production of alkaline protease by a UV mutant of Bacillus polymyxa. Indian Journal of Microbiology 42,155-159.

Mussarat S, Aamer AS, Abdul H, Fariha H. 2000. Influence of culture conditions on production and activity of protease from Bacillus subtilis BS5. Pakistan Journal of Botany 40, 2161-2169.

NorrisJL, Porter NA, Caprioli RM, 2003. Mass spectrometry of intracellular and membrane proteins using cleavable detergents. Analytical Chemistry 75, 6642-6647 DOI org/10.1021/ac034802z.

Prescott LM, Harley JP, Klein DA. 1999. Microbiology. 4th edition, McGraw-Hill companies, New Delhi, pp151-310.

Rao MB, Tanksale AM, Ghalge MS, Desphande VV. 1998. Molecular and biotechnological aspects of microbial proteases.Microbiology, Molecular Biology Review 62, 597-635.

Whitaker DR, Hanson KR, Datta PK. 1963. Improved procedure for preparation and characterization of Myrothecium cellulase. Part 2: Purification Procedure. Canadian Journal of Biochemistry and Physiology 41, 671-696.

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