Paper Details

Phylogenetic relationships of sub-populations of Barbus sharpeyi fish in southwest of Iran using mitochondrial DNA with PCR- sequencing method

Alireza Darabi, Nasser Kashan, Jamal  Fayazi, Mehdi Aminafshar, Mohammad  Chamani

DOI: http://dx.doi.org/10.12692/ijb/5.2.41-46

Int. J. Biosci. 5(2), 41-46. July, 2014. (PDF)

Abstract:

This study was conducted to Investigate cytochrome oxidase I (COI) Region from Barbus sharpeyi mitochondrial DNA (mtDNA), In Other to study genetic and phylogenetic relationships. A total number of 31 samples of fishery farms, jrahi river in shadegan and karkhe river in hovaizeh in southwest of Iran were studied. After DNA(Deoxyribo Nucleic Acid ) extraction, COI Region from mtDNA (aprox. 610 bp) was amplified using COI – 625R and COI – 625F primers. A total number of 25 Amplified PCR)Polymerase Chain Reaction) products was sequenced. Then sequenced samples was Investigated using BioEdit 5.0.9, MEGA5 (Molecular Evolutionary Genetics Analysis Version 5.0.) and Arlequin 3.5 softwares. The average nucleotide Diversity was 0.0286, 0.0785 and 0.0072 respectively. This result showed a low genetic diversity within sub-populations in B.sharpeyi specie. The UPMGA(Unweighted Pair Group Method with Arithmetic Mean) dandrogram which was constructed based on the distance matrix between groups with MEGA5 program. ‏ The most part of genetic variance ( prox. %85.5) in B. sharpeyi was within populations. The obtained results at the present study showed a gene flow between Jrahi and Karoon river was happened. This results suggested that for maintaining of genetic diversity of B. sharpeyi sub-populations at appropriate levels , the exchange and transfer of barbus stocking between Jrahi and other rivers should be avoided and should be used new reproductive stocks.