Paper Details

The effect of plant growth regulators on embryogenic callus induction and regeneration from coleoptile in rice

Hamideh Ghobeishavi, Ebrahim Dorani Uliaie, Seyed Siamak Alavikia, Mostafa Valizadeh

DOI: http://dx.doi.org/10.12692/ijb/5.2.144-150

Int. J. Biosci. 5(2), 144-150. July, 2014. (PDF)

Abstract:

To achieve a repeatable tissue culture system is the first successful step in the genetic engineering of plants. For obtaining this objective in this study potential of coleoptile explant and tow genotype for regeneration were examined. Effect of different treatments on callus induction and regeneration from coleoptile explant was investigated. Significant difference was observed between two cultivars in callus induction and somatic embryogenesis. The plant regeneration was influenced by the genotype as well as composition of the medium. The high frequency plant regeneration was achieved from both cultivars in medium containing 2 mg/L kinetin plus 0.5 mg/L NAA and medium containing 2 mg/L BAP plus 0.5 mg/L kinetin. Regeneration percentage of Neda variety (32.39%) was higher than Nemat variety (20/95%).