Paper Details

The effect of salt stress on seed germination and seedling growth on four Wheat (Triticum aestivum) varieties

Mahnaz Sharifi, Marziyeh Hoseini, Puriya Gharavi-kuchebagh, Sahar Baser-Kouchebagh

DOI: https://dx.doi.org/10.12692/ijb/4.12.73-77

Int. J. Biosci. 4(12), 73-77. June, 2014. (PDF)

Abstract:

The main purpose of the present study was evaluation the effects of salt stress on the seed germination and seedling growth of four wheat varieties. It was conducted in factorial experiment based on completely randomize design (CRD) with three repetitions. Factors were four wheat varieties (Soasel, Shahriyar, Zarin, and Cascogen) and four different concentrations of sodium chloride: 2 g/lit (0.05 M), 5 g/lit (0.12 M), 8 g/lit (0.2 M), 11 g/lit (0.28 M) and control. Salt treatments were applied through the water used for seeds germination, and seedling growth. Seeds were disinfected by dipping them into 5% sodium in disinfected hypochlorite solution for 3 minutes and then were rinsed three times by distilled water. Each experimental unit consisted of one Petri dish containing 25 wheat seeds which were placed in between two special papers. 7 ml of the desired solution was added to each Petri dish and then they all were placed into the growth chambers and kept at 25°C temperature for 14 days. The results of laboratory study showed that the influence of various treatments on germination percentage was significant. 2 g/lit (0.05 M) showed the highest positive effect on germination percentage for Zarin variety. Also, the shortest ratio of radicle/plumule were associated with Zarin type, thus Zarin was identified to be the most tolerant to salt stress as compared to the other three varieties.