Paper Details

Comparison of some DNA isolation methods in Chamomile (Matricaria chamomilla L.)

Amin Azadi, Maryam zandipour, Arghavan Ghamsari, Saeede Khani

DOI: https://dx.doi.org/10.12692/ijb/4.12.301-305

Int. J. Biosci. 4(12), 301-305. June, 2014. (PDF)

Abstract:

There are different methods for DNA extraction in different plants. Quality and quantity of extracted DNA are important parameters. The presence of inhibitory compounds such as polyphenols and secondary metabolites can affect direct or indirect on DNA extraction process and cause reduction of DNA concentration and impurities of extracted DNA. In order to compare available method for DNA extraction in chamomile, a factorial experiment was conducted with a completely random design in three replicates. Factors were different DNA extraction methods (five common methods) and leaf tissue (young/mature). Quality and quantity of extracted DNA was evaluated with the ratio of absorbance at 260 to 280 nm and agarose gel electrophoresis. Method Doyle and Doyle (1987) had the highest DNA concentration among others with 141ng/µl. Quality and quantity of extracted DNA was higher in young leaves than mature.