Chemical and biological investigations of the leaves of Gynura procumbens
Paper Details
Chemical and biological investigations of the leaves of Gynura procumbens
Abstract
The crude n-hexane (HX), dichloromethane (DCM), methanol (ME) and ethyl acetate (EA) extracts of the leaves of Gynura procumbens were subjected to antioxidant, antibacterial and antifungal activities and cytotoxicity against brine shrimp nauplii. The DCM and EA extracts exhibited mild antimicrobial activities, whereas, HX and ME extracts did not show any sort of sensitivity. On the other hand, the brine shrimp lethality with LC50 values was 70.71, 0.78, 4.42 and 59.46 µg/mL for HX, DCM, ME and EA extracts, respectively indicates the presence of potent bioactive compounds. At the same time, among the four crude extracts, only ME and HX extracts showed potential antioxidant activity with IC50 values of 20.35 and 48.0µg/mL. A pure compound was isolated from HX extract and the structure of the compound was elucidated as stigmasterol by means of 1H NMR spectroscopy.
Perry LM. 1980. Medicinal Plants of East and Southeast Asia: Attributed Properties and Uses. 1st Edn., The MIT Press, Cambridge, Massachusetts and London, ISBN: 0-262-16076-5, 334- 360.
Nawawi A, Nakamura N, Hattori M, Kurokawa M, Shiraki K. 1999. Inhibitory effects of Indonesian medicinal plants on the infection of herpes simplex virus type 1. Phytotherapy Research 13, 37- 41. http://dx.doi.org/10.1002/(SICI)1099-1573(199902)13:1<37::AID-PTR382>3.0.CO;2-S/pdf
Li WL, Ren BR, Zhuo M, Hu Y, Lu CG. 2009. The anti-hyperglycemic effect of plants in genus gynura cass. The American Journal of Chinese Medicine 37, 961- 966. http://www.ncbi.nlm.nih.gov/pubmed/19885955
Akowuah GA, Sadikun A, Mariam A. 2002. Flavonoid identification and hypoglycemic studies of butanol fraction from Gynura procumbens. Pharmaceutical Biology 40, 405- 410. http://dx.doi.org/10.1076/phbi.40.6.405.8440
Zhang XF, Tan BKH. 2000. Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and streptozotocin-induced diabetic rats. Singapore Medical Journal 41, 9- 13. http://cat.inist.fr/?aModele=afficheN&cpsidt=269964
Iskander MN, Song Y, Coupar IM, Jiratchariyakul W. 2002. Antiinflammatory screening of the medicinal plant Gynura procumbens. Plant Foods for Human Nutrition 57, 233- 244. http://dx.doi.org/10.1023/A:1021851230890
Agustina D, Haryana SM, Supartinah A. 2006. Anticarcinogenesis effect of Gynura procumbens (Lour) merr on tongue carcinogenesis in 4NQO-induced rat. Dental Journal (Maj. Ked. Gigi) 39, 126-132.
Hoe SZ, Kamaruddin MY, Lam SK. 2006. Inhibition of angiotensin-converting enzyme activity by a partially purified fraction of Gynura procumbens in spontaneously hypertensive rats. Medical Principles and Practice 16, 203- 208. http://dx.doi.org/10.1159/000100391
Kim MJ, Lee HJ, Wiryowidagdo S, Kim HK. 2006. Antihypertensive effects of Gynura procumbens extract in spontaneously hypertensive rats. Journal of Medicinal Food 9, 587- 590. http://dx.doi.org/10.1089/jmf.2006.9.587
Lee HJ, Lee B, Chung J, Wiryowidagdo S, Chun W, Kim S, Kim S, Choe M. 2007. Inhibitory effects of an aqueous extract of gynura procumbens on human mesangial cell proliferation. Korean Journal of Physiology & Pharmacology 11, 145- 148.
Puangpronpitag D, Chaichanadee S, Naowaratwattana W, Sittiwet C, Thammasarn K, Luerang A, Kaewseejan N. 2010. Evaluation of nutritional value and antioxidative properties of the medicinal plant Gynura procumbens extract. Asian Journal of Plant Sciences 9, 146- 151. http://dx.doi.org/10.3923/ajps.2010.146.151
Rosidah MFY, Sadikun A, Asmawi M. 2008. Antioxidant potential of Gynura procumbens. Pharmaceutical Biology 46, 616- 625. http://dx.doi.org/10.1080/13880200802179642
Mahmood AA, Mariod AA, Al-Bayaty F, Abdel-Wahab SI. 2010. Anti-ulcerogenic activity of Gynura procumbens leaf extract against experimentally-induced gastric lesionsin rats. Journal of Medicinal Plant Research 4, 685- 691. http://indianmedicine.eldoc.ub.rug.nl/root/M3/161 m/
Rosidah MFY, Sadikun A, Ahmad M, Akowuah GA, Asmawi MZ. 2009. Toxicology evaluation of standardized methanol extract of Gynura procumbens. Journal of Ethnopharmacology 123, 244- 249. http://dx.doi.org/10.1016/j.jep.2009.03.011
Akowuah GA, Sadikun A, Mariam A. 2002. Flavonoid identification and hypoglycemic studies of butanol fraction from Gynura procumbens. Pharmaceutical Biology 40, 405- 410. http://dx.doi.org/10.1076/phbi.40.6.405.8440
Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology 45, 493- 496.
Meyer BN, Ferringni NR, Puam JE, Lacobsen LB, Nichols DE, McLaughlin JL. 1982. Brine shrimp: a convenient general bioassay for active constituents. Planta Medica 45, 31- 34. http://dx.doi.org/10.1055/s-2007-971236
Brand-Williams W, Cuvelier ME, Berset C. 1995. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology 28, 25-30. http://dx.doi.org/10.1016/S0023-6438(95)80008-5
Khan RI. 1991. Natural Product: A Laboratory Guide, 2nd Ed. Academic Press, N.Y, USA.
A. F. M. Mustafizur Rahman, Md. Sharif Al Asad (2013), Chemical and biological investigations of the leaves of Gynura procumbens; IJB, V3, N4, April, P36-43
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