Production of eleutherosides, total phenolics and total favonoids from somatic embryos of Siberian ginseng affected by different aeration volume in bioreactor
Paper Details
Production of eleutherosides, total phenolics and total favonoids from somatic embryos of Siberian ginseng affected by different aeration volume in bioreactor
Abstract
Physical culture condition plays crucial roles in mass production of somatic embryos in bioreactor. The effects of supplied aeration volume in a balloon type bubble bioreactor (BTBB) to produce somatic embryos were investigated in this study. Best response in case of fresh weight (FW), dry weight (DW) and growth ratio (GR) were observed when input aeration volume were changed every 10 days as follows 0.05/0.1/0.2/0.3 vvm rather than constant aeration volume. Maximum FW 99.28 g/l and DW 11.35 g/l were found in above aeration volume although the highest growth ratio was noticed when 0.05 vvm constant aeration volume was applied. Highest amount of total eleutherosides (108.51 µg/g DW) were produced while aeration volume was changed in every 10 days but higher chlorogenic acid was found while constant aeration volume was 0.01 vvm (1.05 mg/g DW). Pattern of total phenolics and total flavonoids production also revealed that, maximum amount of those metabolities were produced when input air was changed every 10 days. The results indicated that input aeration volume has a potential role on somatic embryos production and secondary metabolites accumulation in bioreactor.
Brekhman II, Dardymov IV. 1969. New substances of plant origin which increase nonspecific resistance. Annual Review of Pharmacology 9, 415. http://dx.doi.org/10.1146/annurev.pa.09.040169.00 2223
Choi YE, Yang DC, Yoon ES. 1999. Rapid propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of germinating zygotic embryos. Plant Cell Tissue and Organ Culture 58, 93-97. http://dx.doi.org/doi10.1023/A:1006318928684
De Feria M, Jime´ nez E, Barbo´ n R., Capote A, Cha´ vez M, Quiala E. 2003. Effect of dissolved oxygen concentration on differentiation of somatic embryos of Coffea arabica cv. Catimor 9722. Plant Cell Tissue and Organ Culture 72, 1–6. http://dx.doi.org/10.1016/j.scienta.2008.05.013
Eschbach LF, Webster MJ, Boyd JC. 2000. The effect of Siberian ginseng (Eleutherococcus senticosus) on substrate utilization and performance. International Journal of Sports Nutrition and Exercise Metabolism 10, 444-451.
Folin O, Ciocalteu V. 1927. On tyrosine and tryptophane determination in proteins. Journal of Biological Chemistry 27, 627-650.
Gaffney BT, Hugel HM, Rich PA. 2001. The effects of Eleutherococcus senticosus and Panax ginseng on steroidal hormone indices of stress and lymphocyte subset numbers in endurance athletes. Life Sciences 70, 431-442.
Gao JW, Lee JM. 1992. Effect of oxygen supply on the suspension culture of genetically modified tobacco cells. Biotechnology Progress 8, 285-290.
Gui Y, Guo Z, Ke S, Skirvin RH. 1991. Somatic embryogenesis and plant regeneration in Acanthopanax senticosus. Plant Cell Reports 9, 514-516. http://dx.doi.org/10.1007/BF00232108
Hohe A, Winkelmann T, Schwenkel HG. 1999. The effect of oxygen partial pressure in bioreactors on cell proliferation and subsequent differentiation of somatic embryos of Cyclamen persicum. Plant Cell Tissue and Organ Culture 59, 39-45.
Isoda S, Shoji J. 1994. Studies on the cultivation of Eleutherococus senticosus Maxim. II On the germination and rising of seedling. Nature Medicine 48, 75-81.
KimYS. 2002. Production of ginsenoside through bioreactor cultures of adventitious roots in ginseng (Panax ginseng C.A. Meyer). Ph.D. thesis, Chungbuk National Univesity, Cheongju, Korea.
Kurata K, Shimazu T. 2006. Effects of dissolved oxygen concentration on somatic embryogenesis. In: Gupta SD, Ibaraki Y (eds.), Plant Tissue Culture Engineering. Springer, Dordrecht 339-353.
Leckie F, Scragg AH, Cliffe KC. 1991. An investigation into the role of initial kLa on the growth and alkaloid accumulation by cultures of Catharanthus roseus. Biotechnology and Bioengineering 37, 364-370.
Lee WT. 1979. Distribution of Acanthopanax plants in Korea. Korean Journal of Pharmacology 10,103-107.
Lindsey K, Yeoman MM. 1983. The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. Journal of Experimental Botany 34, 1055-1065. http://dx.doi.org/10.1093/jxb/34.8.1055
Murashige T, Skoog F. 1960. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
Namdev PK, Dunlop AE. 1995. Shear sensitivity of plant cells in suspensions, present and future. Applied Biochemistry and Biotechnology 54, 109-131.
Patrick NY, Arnason JT, Anwang DVC. 1998. An Improved extraction procedure for the rapid, quantitative high-performance liquid chromatographic estimation of the main eleutherosides (B and E) in Eleutherococcus senticosus (Eleuthero). Phytochemical Analysis 9, 291-295.
Paek KY, Chakrabarty D, Hahn EJ. 2005. Application of bioreactor system for large-scale production of horticultural and medicinal plants. Plant Cell Tissue and Organ Culture 81, 287-300. http://dx.doi.org/10.1007/s11240-004-6648-z
Paek KY, Chakrabarty D. 2003. Micropropagation of woody plants using bioreactor. In: Jain SM, Ishii K, eds. Micropropagation of woody trees and fruits. Kluwer Academic Publisher, The Netherland, 735-756. (doi http://dx.doi.org/10.1007/978-94-010-0125-0_25
Pan ZW, Wang HQ, Zhong JJ. 2000. Scale-up study on suspension cultures of Taxus chinensis cells for production of taxane diterpene. Enzyme and Microbial Technology 27, 714-723.
Schlatmann JE, Fonck E, ten Hoopen HJG, Heijnen JJ. 1994. The negligible role of carbon di oxide and ethylene in ajmalicine production by Catharanthus roseus cell suspensions. Plant Cell Reports 14, 157-160.
Schlatmann JE, Nuutila AM, van Gulik WM, ten Hoopen HJG, Verpoorte R, Heijnen JJ. 1993. Scaleup of ajmalicine production by plant cell culture of Catharanthus roseus. Biotechnology and Bioengineering 41, 253-262.
Schmolz MW, Sacher F, Aicher B. 2001. The synthesis of Rantes, G-CSF, IL-4, IL-5, IL-6, IL-12 and IL-13 in human whole-blood cultures is modulated by an extract from Eleutherococcus senticosus L. roots. Phytotherapy Research 15, 268-270. http://dx.doi.org/10.1002/ptr.746
Shohael AM, Chakrabarty D, Yu KW, Hahn EJ, Paek KY. 2005. Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an airlift bioreactor and production of eleutherosides. Journal of Biotechnology 120, 228-236. http://dx.doi.org/10.1016/j.jbiotec.2005.06.010
Shohael AM, Ali MB, Yu KW, Hahn EJ, Islam RI, Paek KY. 2006. Effect of light on oxidative stress, secondary metabolites and induction of antioxidant enzymes in Eleutherococcus senticosus somatic embryos in bioreactor. Process Biochemistry 41, 1179-1185. (doi:10.1016/j.procbio.2005.12.015)
Shohael AM, Chakrabarty D, Ali MB, Yu KW, Hahn EJ, Lee HL, Paek KY. 2006. Enhancement of eleutherosides production in embryogenic cultures of Eleutherococcus sessiliflorus in response to sucrose-induced osmotic stress. Process Biochemistry 41, 512-518. http://dx.doi.org/10.1016/j.procbio.2005.09.005
Shohael AM, Khatun SM, Alam MF, Paek KY. 2013. Effects of Murashige and Skoog medium strength on germination and secondary metabolites production of Eleutherococcus senticosus’s somatic embryos in bioreactor. International Journal of Biosciences 3, 155-163. http://dx.doi.org/10.12692/ijb/3.3.155-163
Smart NJ, Fowler MW. 1981. Effect of aeration on large-scale cultures of plant cells. Biotechnology Letter 3, 171-176.
Su WW. 1995. Bio-processing technology for plant cell suspension cultures. Applied Biochemistry and Biotechnology 50, 189-230.
Takayama S, Akita M. 1994. The type of bioreactors used for shoots and embryos. Plant Cell Tissue and Organ Culture 39, 147-156.
Tao L, Yang Y, Wang Q, You X. 2012. Callose deposition is required for somatic embryogenesis in plasmolized Eleutherococcus senticosus zygotic embryo. Internal Journal Of Molecular Sciences 13, 14115-14126. http://dx.doi.org/10.3390/ijms131114115
Thanh NT, Murthy HN, Yu KW, Jeong CS, Hahn EJ, Paek KY. 2006. Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng. Journal of Plant Physiology 163, 1337-1341.
Vardar-Sukan F. 1985. Dynamics of oxygen mass transfer in bioreactors. Part I: Operating variables affecting mass transfer. Process Biochemistry 20, 181-184.
Vardar-Sukan F. 1986. Dynamics of oxygen mass transfer in bioreactors. Part II: Design variables. Process Biochemistry 21, 40-43.
Yang JL, Zhao B, Seong ES, Kim MJ, Kang WH. 2010. Callus induction and high-efficiency plant regeneration via somatic embryogenesis in Papaver nudicaule L., an ornamental medicinal plant. Plant Biotechnology Reports 4, 261-267.
Yesil-Celiktas O, Gurel A, Vardar-Sukan F. 2010. Large Scale Cultivation of Plant Cell and Tissue Culture in Bioreactors. Transworld Research Network, Kerala. 1-54.
ZhongJJ, Yoshida M, Fujiyama K, Seki T, Yoshida T. 1993. Enhancement of anthocyanine production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. Journal of Fermentation Bioengineering 75, 299-303.
Zimmerman JL. 1993. Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5, 1411-1423.
Ziv M. 2000. Bioreactor technology for plant micropropagation, In: Janick J, Horticultural Reviews, ISBN 0-471-33374-3, John Wiley & Sons, Inc.
Abdullah Mohammad Shohael, Kee Yoeup Paek (2013), Production of eleutherosides, total phenolics and total favonoids from somatic embryos of Siberian ginseng affected by different aeration volume in bioreactor; IJB, V3, N4, April, P213-221
https://innspub.net/production-of-eleutherosides-total-phenolics-and-total-favonoids-from-somatic-embryos-of-siberian-ginseng-affected-by-different-aeration-volume-in-bioreactor/
Copyright © 2013
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0