Physicochemical and phytochemical analysis of Glycyrrhiza glabra root extract

Paper Details

Research Paper 07/09/2025
Views (129)
current_issue_feature_image
publication_file

Physicochemical and phytochemical analysis of Glycyrrhiza glabra root extract

J. Ramalakshmi, P. Vinodhiniand, V. Ramamurthy
J. Biodiv. & Environ. Sci. 27(3), 50-56, September 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

Glycyrrhiza glabra L., also known as licorice, belongs to the Fabaceae family and is one of the most commercially valuable plants worldwide, being used in the pharmaceutical, cosmetic, and food industries, both for its therapeutic benefits as well as for the sweetening properties of the extract. The aim of the study is to analyse the physicochemical and phytochemical characteristics of Glycyrrhiza glabra in aqueous and ethanolic extracts and determine the ash value and extractive value of the drug. Using soxhlet equipment the extract was prepared from powdered Glycyrrhiza glabra drugs for 20 hours using water and ethanol as solvents. The analysis of phytochemical screening of Glycyrrhiza glabra includes tests for carbohydrates, saponins, flavonoids, alkaloids, etc. The percentage yield of ethanol extract is found to be 9.73%. The physicochemical parameters of the aqueous solution and ethanol were analysed and are found to be in limits. The ethanolic extract of Glycyrrhiza glabra showed the presence of alkaloids, flavonoids, tannins, phenols, saponins, carbohydrates, glycosides and phytosterol. The amounts of total flavonoids, total phenol and ascorbic acid were found to be 185.14 mg, 481.47 mg per 1 gm, and 33.81 μg/ml of aqueous extract and 218.92 mg, 507.62 mg per 1 gm, and 42.38 μg/ml of the ethanol extract, respectively. It was found that the phytochemical constituents are very much enriched in the Glycyrrhiza glabra extract and can be used for development of new formulations.

Ainsworth EA, Gillespie KM. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols 2(4), 875–877.

Bysani S, Babu PS, Karthikeyan R. 2017. Proximate, powder microscopic, liquid chromatographic and in-vitro anti-inflammatory activity of marketed athimadhuram churnas. Journal of Medicinal Plants Studies 5(3), 373–383.

Chauhan S, Gulati N, Nagaich U. 2018. Acido glicirrícico: extracción, cribado y evaluación de la propiedad anti-inflamatoria. Ars Pharmaceutica 59(2), 61–67.

Diomede L, Beeg M, Gamba A, Fumagalli O, Gobbi M, Salmona M. 2021. Can antiviral activity of licorice help fight COVID-19 infection. Biomolecules 11, 855.

Fiore C, Eisenhut M, Ragazzi E, Zanchin G, Armanini D. 2005. A history of the therapeutic use of liquorice in Europe. Journal of Ethnopharmacology 99, 317–324.

Katoch R. 2011. Analytical techniques in biochemistry and molecular biology. Springer Science & Business Media

Kokate CK, Purohit AP, Gokhale SB. 2000. Pharmacognosy.

Lim TK. 2005. Glycyrrhiza glabra. In: Edible medicinal and non-medicinal plants. Vol. 10, 354.

Mamedov NA, Egamberdieva D. 2019. Phytochemical constituents and pharmacological effects of licorice: A review. Plant and Human Health: Pharmacology and Therapeutic Uses 3, 1–21.

Medini F, Fellah H, Ksouri R. 2014. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. Journal of Taibah University for Science 8(3), 216–224.

Pastorino G, Cornara L, Soares S, Rodrigues F, Oliveira MBPP. 2018. Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytotherapy Research 32(12), 2323–2339.

Pastorino G, Cornara L, Soares S, Rodrigues F, Oliveira MBPP. 2018. Liquorice (Glycyrrhiza glabra): A phytochemical and pharmacological review. Phytotherapy Research 32, 2323.

Quirós-Sauceda AE, Ovando-Martínez M, Velderrain-Rodríguez GR, González-Aguilar GA, Ayala-Zavala JF. 2015. Licorice (Glycyrrhiza glabra Linn.) oils. In: Essential oils in food preservation, flavor and safety, 523–530.

Sam S. 2019. Importance and effectiveness of herbal medicines. Journal of Pharmacognosy and Phytochemistry 8(2), 354–357.

Sharma VA, Agrawal RC. 2013. Glycyrrhiza glabra – a plant for the future. Mintage Journal of Pharmaceutical and Medical Sciences 2(3), 15–20.

Tan D, Tseng HHL, Zhong Z, Wang S, Vong CT, Wang Y. 2022. Glycyrrhizic acid and its derivatives: Promising candidates for the management of type 2 diabetes mellitus and its complications. International Journal of Molecular Sciences 23, 10988.

Thakur AK, Raj P. 2017. Pharmacological perspective of Glycyrrhiza glabra Linn: A mini-review. Journal of Analytical & Pharmaceutical Research 5, 00156.

Vashist H, Sharma D. 2013. Pharmacognostical aspects of Glycyrrhiza glabra. Asian Journal of Pharmaceutical and Clinical Research 6(4), 55–59.

Wahab S, Annadurai S, Abullais SS, Das G, Ahmad W, Ahmad MF, Kandasamy G, Vasudevan R, Ali MS, Amir M. 2021. Glycyrrhiza glabra (Licorice): A comprehensive review on its phytochemistry, biological activities, clinical evidence and toxicology. Plants 10, 2751.

Related Articles

Protective effect of polyherbal formula nalpamaram on the ethanol-induced toxicity in Hep G2 cell lines

S. Jyothilekshmi, Jini Joseph, J. Biodiv. & Environ. Sci. 27(5), 137-142, November 2025.

Household socio-agricultural profiles and the adoption of crop protection strategies in human-wildlife conflict contexts: Insights from western Côte d’Ivoire around mount Sangbé National Park

Koffi Kouamé Christophe, Ouffoue Affoué Eugénie Naomie, Gagbé Dalié Sylvestre, Beda Alex, J. Biodiv. & Environ. Sci. 27(5), 91-103, November 2025.

Influence of biosynthesized silver nanoparticles on pollen germination and tube growth in Catharanthus roseus (L.) G. Don

Abhijit Limaye, Shreya Mulay, Jidnyasa Jangale, Rasadnya Joshi, Swapna Sathe, Kishor Bhosale, J. Biodiv. & Environ. Sci. 27(5), 85-90, November 2025.

Genetic diversity of parasitoids and entomopathogenic nematodes of Spodoptera frugiperda Smith, 1797 (Lepidoptera: Noctuidae) in Senegal

Farma Fall Babou, Toffène Diome, Mama Racky Ndiaye, Mbacké Sembene, J. Biodiv. & Environ. Sci. 27(5), 69-84, November 2025.

Environmental and socio-economic impacts of pollution by Eichhornia crassipes (Mart.) Solms in the waters of Dams No. 2 and No. 3 in the city of Ouagadougou, Burkina Faso

Florent Y. Lankoande, Jerome T. Yameogo, Asseta Tabsoba, S. E. I. Bama, J. Biodiv. & Environ. Sci. 27(5), 59-68, November 2025.

Evaluation of grains and haulms production of soybean varieties in production areas with high livestock potentiality in Benin

Assouan Gabriel Bonou, Alain Sèakpo Yaoitcha, Serge Aklinon, J. Biodiv. & Environ. Sci. 27(5), 51-58, November 2025.