Identification and association of TCF7L2 gene polymorphism with type 2 diabetes risk in north Indian population
Paper Details
Identification and association of TCF7L2 gene polymorphism with type 2 diabetes risk in north Indian population
Abstract
Type 2 diabetes Mellitus (T2DM) is a metabolic disorder caused by increased insulin secretion and high blood glucose levels. These increased glucose levels lead to various complications. Here, we investigate the case-control study, where we have selected 200 diabetic and 200 healthy participants from the North Indian population. To understand the function of the rs4506565 & rs11196205 single-nucleotide polymorphism of the TCF7L2 gene in T2DM, I used amplification refractory mutation system (ARMS-PCR) and DNA sequencing. The analysis of the rs4506565 and rs11196205 polymorphisms showed a significant association with T2DM when comparing patients with controls (p-values < 0.05). The TT genotype of rs4506565 shows a higher frequency in the diabetes group (49%) compared to the control group (32.5%), with a model of T/T vs. A/T-A/A: 𝜒2=12.01, p=0.0008, OR=1.99 and 95% Cl= 1.33-2.99. Similarly, the CC genotype of rs11196205 was more frequent in the diabetes group (51%) compared to the control group (30%), with a model of C/C vs. C/C-G/G: 𝜒2=18.16, p=0.001, OR=0.41, and 95% Cl=0.27- 0.62 in the North Indian population. Overall, our recent findings suggest that rs4506565 and rs11196205 are positively associated with susceptibility to T2DM.
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