Identification and quantification of viruliferous and non- viruliferous Polymyxa betae
Paper Details
Identification and quantification of viruliferous and non- viruliferous Polymyxa betae
Abstract
Rhizomania, caused by Beet Necrotic Yellow Vein Virus (BNYVV) is transmitted by plasmodiophorid Polymyxa betae. To investigate quantification of virulifeous and non- viruliferous P. betae isolates, different techniques including serological method (DAS- ELISA), PCR- based method and nanobiocensor method have been used. For this purpose, sugar beet susceptible cultivar (Regina) was cultivated in soils of different regions in greenhouse conditions. Six weeks after planting, lateral roots of beets from each soil were visually tested through microscopy and the of P. betae cystosori was seen and the lateral root sap was prepared. Then DAS- ELISA with polyclonal antibody against recombinant expressed fungal glutathione-s- transferase isolates of Shiraz was optimized. Optical density of different samples were calculated for both the vector and the virus using ELISA method. Simultaneously, confirmation of quantitative estimation P. betae in lateral root was conducted by nanobiosensor against vector. Nanobiosensor method was performed based on Florescent Resonance Transfer Energy (FRET) using antibody attached quantom dots and GST conjugated rhodamine. Microscopic results show presence of vector in all soils. BNYVV was found in soils Fars, Khorasan, Hamadan and Kermanshah. In soils of Azarbayjan, Gorgan, Dezfool, Kerman, Karaj and Arak were found no virus. Values of optical density of P. betae in soils with and without virus have no significantly difference. Because of high speed and sensitivity of nanobiosensor, its use for quantitative estimation of P. betae has been advised.
Algar WR, Krull UJ. 2007. Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acid, proteins, and other biological molecules. Annual Bioanalytical Chemistery 391, 1609- 1618.
Asher MJC, Chwarszczynskam DM, Leaman M. 2003. The evaluation of Rhizomania resistant sugar beet for the UK. Annual Applied Biology 141, 101-109.
Blunt S, Asher J, Gilligan C. 1991. Infection of sugar beet by Polymyxa betae in relation to soil temperature. Plant Pathology 40, 257- 67.
Delfosse P, Reddy AS, Legrève A, Thirumala Devi K, Abdurahman MD, Maraite H, Reddy DVR. 2000. Serological Methods for Detection of Polymyxa graminis, an Obligate Root Parasite and Vector of Plant Viruses Phytopathology 90, 537- 545.
Frasco MF, Chaniotakis N. 2009 Semiconductor quantum dots in chemical sensors and biosensors. Sensors 9, 7266–7286. http://dx.doi.org/10.3390/s90907266
Hahn MA, Keng PC, Krauss TD. 2008. Flow cytometric analysis to detect pathogens in bacterial cell mixtures using semiconductor quantum dots. Anal Chemistry 80, 864- 872. http://dx.doi.org/10.1021/ac7018365
Izadpanah K, Hashemi P, Kamran R, Pakniat M, Sahandpour A, Masumi M. 1996 Widespread occurrence of rhizomania-like disease of sugar beet in Fars. Iranian Journal of Plant Pathology 32, 200– 206.
Kingsnorth CS, Asher MJC, Keane GJP, Chwarszczynska DM, Luterbacher MC, Mutasa-Gottgens ES. 2003. Development of a recombinant antibody ELISA test for the detection of Polymyxa betae and its use in resistance screening. Plant Pathology 52, 673–680.
Legreve A. 2003. Phylogenetic analysis of Polymyxa species based on nuclear 5.8S. and internal transcribed spacers ribosomal DNA sequences. Mycology Research 106, 138-47.
McGrann GRD, Grimmer MK, Mutasa-Gottgens EF, Steven M. 2009. Progress towards the understanding and control of sugar beet rhizomania disease. Molecular Plant Pathology 10, 129- 141. http://dx.doi.org/10.1111/j.1364-3703.2008.00514.x
Merz U, Walsh JA, Bouchek-Mechiche K, Oberhansli T, Bittelin W. 2005. Improved immunological detection of Spongospora subterranean. European Journal of Plant Pathology 111, 371-379.
Meunier A, Schmit JF, Stas A, Kutluk N, Bragard C. 2003. Multiple reverse transcription – PCR for simultaneous detection of beet necrotic yellow vein virus, Beet soilborn virus , and Beet virus Q and their vector Polymyxa betae KESKIN on sugare beet. Applied Environmental Microbiology 69, 2356- 2360.
Mutasa ES, Chwarszczynska DM, Adams MJ, Ward E, Asher MJC. 1993. A sensitive DNA probe for the detection of Polymyxa betae in Sugar beet roots and its application in field Studies. Physiologic and Molecular Plant Pathology 47, 303-313.
Mutasa-Gottgens ES, Chwarszczynska DM, Halsey K, Asher MJC. 2000. Specific Polyclonal antibodies for the obligate plant parasite Polymyxa- a targeted recombinant DNA approaches. Plant Pathology 49, 276–287.
Pavli OL, Stevanato P, Biancardi E, Skaracis GN. 2011. Achievements and prospects in breeding for rhizomania resistance in sugar beet. Field Crops Research 122, 165- 172.
Rush CM. 2003. Ecology and epidemiology of Benyvirus and plasmodiophorid vectors. Annual Review Phytopathology 41, 567–592.
Safarpour H, Safarnejad MR, Basirat M, Hasanzadeh F, Kamiab F. 2012a. Development of a specific serological kit for detection of Polymyxa betae, transmittingagent of sugar beet rhizomania disease. Journal of Food, Agriculture and Environment 10, 729-732.
Safarpour H, Safarnejad MR, Tabatabaie M, Mohsenifar A, Rad F, Shahryari F, Hasanzadeh F. 2012b. Development of a quantum dots FRET-Based biosensor for efficient detection of Polymyxa betae. Canadian Journal of Plant Pathology 34, 507–515.
Sohi H, Maleki M. 2004. Evidence for presence of types A and B of Beet necrotic yellow vein virus (BNYVV) in Iran. Virus Genes 29, 353–358
Tuitert G. 1990. Assessment of the inoculum potential of Polymyxa betae and Beet Necrotic Yellow Vein Virus in soil using the most probable number method. Netherlands Journal of Plant Pathology 96, 331-41.
Wakeham AJ, White JG. 1996. Serological detection in soil of Plasmodiophora brassicae resting spores. Physiological and Molecular Plant Pathology 48, 289-303.
Wisler G, Lewellen R, Sears J, Wasson J, Liu H-Y. 2003. Intraction between Beet Necrotic Yellow Vein Virus and Beet Soilborn Mosaic Virus and their effect on virus levels in sugar beet. Proc. Symp. Int. Work. Group on Plant Viruses with Fungal Vectors, 5th.Denever: American Society of Sugar Beet Technology. pp. 56-59.
Yilmaz K. 2010. Interaction between Beet Necrotic Yellow Vein Virus and Beet Soilborne Virus in different sugarbeet cultivars. Anadolu Journal of Agricultural Science 25, 68-74.
Fatemeh Hassanzadeh Davarani, Saeed Rezaee, Seyed Bagher Mahmoudi, Peyman Norouzi, Mohammad Reza Safarnejad, 2013. Identification and quantification of viruliferous and non- viruliferous Polymyxa betae. Int. J. Biosci., 3(6), 165-171.
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