Antimicrobial resistance profiling and molecular characterization of a multidrug-resistant Salmonella enterica serovar Typhimurium from poultry environments in Bangladesh
Paper Details
Antimicrobial resistance profiling and molecular characterization of a multidrug-resistant Salmonella enterica serovar Typhimurium from poultry environments in Bangladesh
Abstract
The emergence of multidrug-resistant (MDR) Salmonella enterica in poultry production systems poses a significant threat to animal health, food safety, and public health worldwide. This study investigated the occurrence, antimicrobial resistance patterns, and molecular identity of zoonotic Salmonella spp. isolated from poultry environments in Bangladesh. A total of 60 samples, including cloacal swabs, tracheal swabs, fecal samples, and eggshell surfaces, were collected from poultry farms and analyzed using conventional culture-based methods and MALDI-TOF mass spectrometry. Salmonella spp. were detected in three samples, corresponding to an overall prevalence of 5%. Antimicrobial susceptibility testing of five Salmonella isolates, including three field isolates and two reference strains, was performed against 20 antibiotics representing multiple classes following CLSI guidelines. In contrast, most isolates exhibited broad susceptibility, one strain, designated Salmonella sp. ABD-261, displayed a multidrug-resistant phenotype, showing resistance to trimethoprim, gentamicin, tetracycline, and colistin sulfate. Molecular identification based on 16S rRNA gene sequencing and phylogenetic analysis confirmed ABD-261 as Salmonella enterica serovar Typhimurium. Notably, phylogenetic reconstruction revealed genetic divergence from closely related reference strains, suggesting potential evolutionary distinctiveness. The detection of an MDR zoonotic S. enterica serovar Typhimurium strain in poultry environments underscores the ongoing risk of antimicrobial resistance dissemination through the food chain. These findings highlight the importance of continuous surveillance, prudent antibiotic use, and strengthened biosecurity measures to mitigate the public health impact of poultry-associated Salmonella in Bangladesh.
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Rashna Islam, Rubaya, Jahangir Alam, Anjuman Ara Bhuyan, Md. Abdul Alim, M. M. Kamal Hossain, Mir Rowshan Akter, Md. Sagir Ahmed, Shohel Mahmud*, 2026. Antimicrobial resistance profiling and molecular characterization of a multidrug-resistant Salmonella enterica serovar Typhimurium from poultry environments in Bangladesh. Int. J. Biosci., 28(2), 201-209.
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