Solvents’ influence on polyphenolic compound extractions from Lippia multiflora leaves (Mold, 1949), and their antioxidant activity

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Research Paper 14/05/2026
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Solvents’ influence on polyphenolic compound extractions from Lippia multiflora leaves (Mold, 1949), and their antioxidant activity

Kelemin Awa Koné*, Tagouèlbè Tiho, Mariam Sanogo, Casimir Kekou, Kouassi Hervé Tani
Int. J. Biosci. 28(5), 109-116, May 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

The plants are the main source of bioactive compounds for new therapeutic strategy developments for numerous diseases’ treatments. The study determined Lippia multiflora leaves’ total polyphenol, and condensed tannin contents, and their antioxidant activities. So, aqueous, 70%Eth, and 70%Met solvents (alcohol/distilled water, v/v, 70/30) were prepared with ethanol and methanol, respectively. Then, these solvents were used to extract Lippia multiflora dried leaves. Thereafter, the phytochemical screening was performed to identify the groups of compounds. The extract total polyphenol contents were performed through Folin-Ciocalteu method, and condensed tannins were determined in the presence of sulfuric acid. Furthermore, the dry extract (DE) antioxidant activity was determined by using DPPH method. As a result, the total polyphenol and condensed tannin contents were 365±5; 635±5; 525±15 mg GAE/g.DE, and 105.14±3.36; 128.94±5.59; 143.36±9.35 mg Cat.E/g.DE, for the aqueous, 70%Eth and 70%Met extracts, respectively. Additionally, the inhibition rates ranged from 3.95% to 60.69% for the distilled water, from 12.94% to 82.94% for 70%Eth, and from 17.54% to 84.78% for 70%Met extracts. So, 70%Eth was more efficient for Lippia multiflora bioactive compounds extraction, compared to 70%Meth, and distilled water.

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