Assessment of physicochemical factors and partial screening of the biosurfactant producing bacteria in marine water of Kerala
Paper Details
Assessment of physicochemical factors and partial screening of the biosurfactant producing bacteria in marine water of Kerala
Abstract
This research examined the physico-chemical characteristics and cultivable bacterial diversity of a marine water samples collected from Chombala of kerala and also assessing the potential for biosurfactant production. The physical parameters indicated (Alkalinity (total), NH₃/NH₄⁺, Ca²⁺, Cl⁻, Dissolved oxygen, Electrical conductivity, Mg²⁺, NO₃⁻, NO₂⁻, pH, PO₄³⁻, Salinity, SiO2, Temperature, TDS, Total hardness & Turbidity. A total of fifteen bacterial strains were isolated, characterized morphologically, biochemically and identified as Bacillus sp. (40%), along with Vibrio sp., Pseudomonas aeruginosa, Halomonas sp., Serratia marcescens, among others. The biosurfactant screening methods employed (Including Drop Collapse, Oil Spread, Atomized Oil Spray, Microplate Assay, Tilting Slide & Emulsification (24h), Hemolytic Assay, CTAB-MB Plate, Hydrocarbon Overlay and Aggregation) revealed that 10 strains (67%) tested positive, predominantly consisting of Bacillus and Pseudomonas species. The oligotrophic and saline environment supported a diverse community of halotolerant bacteria with significant biosurfactant production potential, underscoring implications for bioremediation and aquaculture.
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P. P. Jasitha, M. Kannahi*, 2026. Assessment of physicochemical factors and partial screening of the biosurfactant producing bacteria in marine water of Kerala. J. Biodiv. Environ. Sci., 29(1), 92-100.
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