Responses of slenderleaf rattlebox (Crotalaria ochroleuca) to water deficit
Paper Details
Responses of slenderleaf rattlebox (Crotalaria ochroleuca) to water deficit
Abstract
Water deficit affects plant growth and development thus affecting crop productivity. It is the major a biotic stress experienced in the vast semi arid and arid regions of Kenya and the world as a whole. Water deficit mainly causes reduction in plant growth and development. Crotalaria ochroleuca is an important indigenous leafy vegetable which is a legume grown and consumed in Kenya and Tanzania and other parts of Africa. The slender leaf rattlebox is very rich in protein, calcium, iron, Beta carotene and ascorbic acid and therefore can contribute to good human health therefore increased production of this vegetable should be used to solve the problem of malnutrition in the country and also act as food security. A study was conducted to evaluate the responses of slender leaf rattlebox to Water deficit. The study as carried out at the University Botanic Garden. Twenty seeds of slender leaf rattlebox were arranged in a randomized complete block design (RCBD) with six treatments and four replications. After 15 days of germination, the plants were thinned up to four plants per pot and subjected to six treatments viz: watering daily (U), watering after 3 days (V), watering after 6 days (W), watering after 9 days (X), watering after 12 days (Y) and watering after 15 days (Z). Data on growth parameters (shoot height, leaf length, leaf width and number of leaves per plant) were collected on weekly basis. Leaf area, shoot/root fresh and dry weights, leaf chlorophyll concentration at different water regimes was also determined. Data collected was subjected to Analysis of variance (ANOVA) using CO-STAT statistical package and treatment means were separated and compared using least significance difference (LSD). The results show that Crotalaria ochroleuca seedlings are very sensitive to water deficit conditions. Water deficit significantly (P ≤ 0.05) decreased the shoot heights, leaf number, leaf area, shoot and root fresh and dry weights and chlorophyll content in the Crotalaria ochroleuca seedlings. The study indicated 55 % reduction in shoot height, 90 % reduction in leaf area, 71% in fresh weight and 90% reduction in dry weights at water deficit treatments compared to the control plants. It may be recommended that for proper growth and development of Crotalaria ochroleuca plants, daily watering or at least once in three days should be carried out.
Abayomi YA, Fadayomi O, Babatola JO, Tian G. 2001. Evaluation of selected Legume cover crops for biomass production, dry season survival and soil fertility improvement in a moist savanna location in Nigeria. Journal of Tropical Crop Science and Production 9, 615–628.
Abukutsa-Onyango M. 2007. Response of slenderleaf (Crotalaria brevidens benth) to inorganic nitrogen application. African Journal of Food Agriculture Nutrition and Development 7(3), 1-10.
Akcay U, Ercan O, Kavas M, Yildiz I, Yilmaz C, Oktem HA, Yucel M. 2010. Drought-induced oxidative damage and antioxidant responses in peanut (Arachis hypogaea L.) seedlings. Plant Growth Regulation 6, 21–28.
Ashraf M, O’Leary JW. 1996. Effects of drought stress on growth, water relations and gas exchange of two lines of sunflower differing in degree of salt tolerance. International Journal of Plant Sciences 157, 729-732.
Bimpong IK, Serraj R, Chin JH, Mendoza EM, Hernandez J, Mendioro MS. 2011. Determination of genetic variability for physiological traits related to drought tolerance in African rice. Plant Breeding and Crop Science 3(4), 60-67.
Blum A. 1996. Crop responses of drought and the interpretation of adaptation. Plant Growth Regululation 20, 135-148.
Chaves MM, Pereira JS, Maroco J, Rodrigues ML, Ricardo CP, Osorio ML, Carvalho I, Faria T, Pinhero C. 2002. How plants cope with water stress in the field? photosynthesis and growth. Annals of Botany 89, 907-916.
Daimon H, Takada S, Ohe M, Mimoto H. 1995. Interspecifc differences in growth and nitrogen uptake among Crotalaria species. Japanese Journal of Crop Science 64, 115–120.
Deblonde PMK, Ledent JF. 2001. Effects of moderate drought conditions on green leaf number, stem height, leaf length and tuber yield of potato cultivars. European Journal of Agronomy 14, 31-41.
Emmam Y, Shekoofa A, Salehi F, Jalali AH. 2010. Water stress effects on two common bean cultivars with contrasting growth habitats. American-Eurasian Journal of Agriculture and Environmental Science 9(5), 495-499.
Farooq M, Basra SMA, Wahid A, Cheema ZA, Cheema MA, Khaliq A. 2008. Physiological role of exogenously applied glycine betaine in improving drought tolerance of fine grain aromatic rice (Oryza sativa L.). Journal of Agronomy and Crop Science 194, 325–333.
Farooq MA, Wahid A, Kobayashi N, Fujita D, Basra SM. 2009. Plant drought stress effects, mechanisms and management. Agronomy Sustainable Development 29, 185-212.
Florence H, Mary KW, Abuksa-Onyango M. 2010. Copper and ascorbic acid content of cooked african indigenous vegetables. Conference on International Research on Food Security, Natural Resource Management and Rural Development Tropentag 201ETH Zurich, September 14 – 16, 2010.
Jaleel CA, Manivannan P, Lakshmanan GM, Gomathinayagam M, Panneerselvam R. 2008. Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Colloids Surf B: Biointerfaces 61, 298–303.
Jaleel CA, Manivannan P, Wahid A, Farooq M, Jasim H, Somasundaram R, Panneerselvan R. 2009. Drought stress in plants: A review of morphological characteristics and pigment composition. International Journal of Agriculture and Biology 11, 100-105.
Kiani SP, Maury P, Sarrafi A, Grieu, P. 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annus L.) under well watered and water stressed conditions. Plant Science 175, 565-573.
Kumar A, Singh DP, 1998. Use of physiological indices as a screening technique for drought tolerance in oilseed Brassica species. Annals of Botany 81, 413-420.
Long SP, Humphries S, Falkowski PG. 1994. Photoinhibition of photosynthesis in nature. Annual Review of Plant Physiology 45, 633-662.
Mafakheri A, Siosemardeh A, Bahramine JS, Struik PC, Sohrabi Y. 2010. Effects of drought stress on yield, proline and chlorophyll contents in three chicken pea cultivars. Australian Journal of Crop Science 4(8), 580-585.
Martinez JP, Silva H, Ledent JF, Pinto M. 2007. Effects of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaseolus vulgaris L.). European Journal of Agronomy 26, 30-38.
Mkiwa FEJ, Sarwatt SV, Lwoga AB, Dzowela BH. 1990. Nutritive value of Crotalaria ochroleuca: chemical composition and in vitro dry matter digestibility at different stages of growth. In PANESA/ARNAB (Pastures Network for Eastern and Southern Africa/African Research Network for Agricultural By-products). 1990. Utilization of research results on forage and agricultural by-product materials as animal feed resources in Africa. Proceedings of the first joint workshop held in Lilongwe, Malawi, 5-9 December 1988. PANESA/ARNAB, Addis Ababa, Ethiopia. 833 pp..
Muthomi J, Musyimi DM. 2009. Growth responses of African nightshades (Solanum scabrum. Mill). Agricultural and Biological Sciences 4(5), 24-31.
Mwai GN. 2001. Growth response of spider plant (Cleome gynandra L.) to salinity, Msc. Thesis. Maseno University, Kenya.
Nadler A, Bruvia H. 1998. Physiological responses of potato plants to soil salinity and water deficit. Plant Science 137, 43 – 51.
Nam NH, Chauhan YS, Johansen C. 2001. Effects of timing of drought stress on growth and grain yield of extra- short duration pigeon pea lines. Agricultural Science 136, 179-189.
Nyalemegbe KK, Osakpa TY, Danso SKA. 2011. Ecological considerations in the selection of leguminous plants as cover crops on the vertisols of the Accra plains in Ghana. Journal of the Ghana Science Association 13, 1-4.
Polhill RM. 1982. Crotalaria in Africa and Madagascar. Royal Botanic Gardens Kew, A.A. Balkema (eds), Rotterdam, Pp. 1–89.
Prasad KN, Shiramurthy GR, Aradhya SM. 2008. Ipomea aquatica, An underutilized green leaf vegetable. International Journal of Botany 1, 123-129.
Reddy AR, Chaitanya KV, Vivekanandan M. 2004. Drought induced responses of photosynthesis and antioxidant metabolism in higher plants. Plant Physiology 161, 1189-1202.
Rosales-serna R, Konani J, Shibata JA, Kelly JD. 2004. Biomass distribution, maturity acceleration and yield in drought stressed common bean cultivars. Field Crops Research 85, 203-211.
Salisbury B, Ross W. 1992. Plant physiology, 4th edition, Wadsworth, Belmont, California. Pp 580 – 585.
Samba RT, Sylla SN, Neyra M, Gueye M, Dreyfus B, Ndoye I. 2002. Biological nitrogen fixation in Crotalaria species estimated using the N isotope dilution method. African Journal of Biotechnology 1 (1), 17–22.
Sarwatt SV, Mkiwa FEJ. 1987. The current status of knowledge on the feed value of Crotalaria species in Tanzania. In Proceedings of the 3rd PANESA workshop held in Arusha, Tanzania 27th-30th April, 1987.
Schippers RR. 2004. Crotalaria ochroleuca G. Don In: Grubben, G.J.H. & Denton, O.A. (Eds). PROTA 2: Vegetables/Légumes. PROTA, Wageningen, Netherlands.
Shao HB, Chu LY, Jaleel CA, Zhao CX. 2008. Water-deficit stress-induced anatomical changes in higher plants. Competes Rendus Biologies 331, 215– 225.
Sikuku PA, Onyango JC, Netondo GW. 2012. Physiological and biochemical responses of Five NERICA rice varieties (Oryza sativa L.) to water deficit at vegetative and reproductive stage. Agriculture and Biology Journal of North America 3(3), 93 – 104.
Tahir MH, Mehid SS. 2001. Evaluation of open pollinated sunflower (Helianthus annus L) populations under water stress and normal conditions. International Journal of Agriculture and Biology 3, 236-238.
Vurayai R, Emongor V, Moseki B. 2011. Physiological responses of Bambara groundnut to short periods of water stress during different development stages. Asian Journal of Agriculture Science 3(1), 37-43.
Webber M, Barnett J, Finlayson B, Wang M. 2006. Pricing China’s Irrigation Water. Working Paper, School of Anthropology, Geography and Environmental Studies, The University of Melbourne, Victoria, Australia.
Wood AJ, Roper J. 2000. A simple and non-destructive technique of measuring plant growth and development. American Biology 62, 215-217.
Yawson DO, Mensa B, Armah FA, Afrifa EK. 2011. Water requirement of sunflower (Helianthus annus L.) in a tropical humid coastal savanna zone. Agricultural and Biological Sciences 6(1), 1-8.
Zhao CX, Guo LY, Jaleel A,Yang HB. 2008. Prospects for dissecting plant-adaptive molecular mechanisms to improve wheat cultivars in drought Environments. Competes Rendus Biologies 331, 579– 586.
P.A. Sikuku, D.M. Musyimi, S. Kariuki, S.V. Okello (2013), Responses of slenderleaf rattlebox (Crotalaria ochroleuca) to water deficit; JBES, V3, N12, December, P245-252
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