Effects of raw rock phosphate rich in heavy metals on oxidative stress markers in layers
Paper Details
Effects of raw rock phosphate rich in heavy metals on oxidative stress markers in layers
Abstract
The present study was planned to evaluate the effects of various concentrations of raw rock phosphate (RRP) rich in heavy metals on oxidative stress markers in blood samples obtained from laying hens. A total of 192 commercial laying hens of the same weight were allocated under the Completely Randomized Design to 6 treatments with 4 replicate cage boxes of 10 chicks each. The birds were maintained in cages for a period of 21 to 45 weeks of age. The RRP [A representative sample from Tarnawai-Lagarban, Danna and Kakul at Khyber Pakhtoon Khawa, Pakistan] was incorporated into a standard diet based on corn, rice broken, rice polishing, soya bean meal, sunflower meal and corn gluten meal (60%) to replace the amount of P provided by the DCP at 0, 25, 50, 75, and 100% level. A control diet was formulated without phosphate. Oxidative stress parameters (TAS, catalase, SOD and GSH-Px) showed decreasing (P<0.05) trend in birds fed raw rock phosphate-based diets as compared to those birds fed control (bone meal) and 100% DCP diets, while TOS and MDA values in birds fed with raw rock phosphate-based diets increased than those of control and 100% DCP diets. It may be concluded that raw rock phosphate-based diets at 75 and 100% replacing DCP may cause buildup of free radicals in the serum, enrichment of lipid peroxidation and hampering the activities of antioxidant enzymes which accordingly stimulates oxidative stress and damages the antioxidant function in hens.
Chen JJ, Yu BP. 1994. Alteration in mitochondrial membrane fluidity by lipid peroxidation products. Free Radical Biology and Medicine 17, 411-418. DOI: 10.1016/0891-5849(94)90167-8
Chen T, Cui HM, Cui Y, Bai CM, Gong T. 2011. Decreased antioxidase activities and oxidative stress in the spleen of chickens fed on high-fluorine diets. Human and Experimental Toxicology 30, 1282-1286.
Clairbone A. 1985. In: Greenwald RA. (Ed.), Catalase Activity. CRC Handbook of Methods in Oxygen Radical Research. CRC Press, boca Raton, FL.
Deng Y, Hengmin C, Xi P, Jing F, Zhicai Z, Junliang D, Qin LK. 2014. Effects of high dietary fluoride on serum biochemistry and oxidative stress parameters in broiler chickens. Health 6, 1840-1848.
Erel O. 2004. A novel automated method to measure total antioxidant response against potent free radical reactions. Clinical Biochemistry 37, 112-119.
Erel O. 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry 38, 1103-1111.
Halliwell B, Chirico S. 1993. Lipid peroxidation: Its mechanism, measurement, and significance. American Journal Clinical Nutrition 57, 715-724.
Halliwell B, Gutteridge J. 1999. Free radicals in biology and medicine, 3rd ed. Oxford University Press, USA.
Hoffman DJ, Heinz GH, Sileo L, Audet DJ, Campbell JK, LeCaptain LJ. 2000. Developmental toxicity of lead-contaminated sediment to mallard ducklings. Archive of Environmental Contamination and Toxicology 39, 221-232.
Hoffman DJ, Spalding MG, Frederick PC. 2005. Subchronic effects of methyl- mercury on plasma and organ biochemistries in great egret nestlings. Environmental Toxicology and Chemistry 24, 3078-3084.
Huang YL, Cheng SL, Lin TH. 1996. Lipid peroxidation in rats administrated with mercuric chloride. Biological Trace Element Research 52, 193-206.
Kohen R, Nyska A. 2002. Oxidation of biological systems: Oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicologic Pathology 30, 620-650.
Liu GY, Chai CY, Cui L. 2003. Fluoride Causing Abnormally Elevated Serum Nitric Oxide Levels in Chicks. Environmental Toxicology and Pharmacology 13, 199-204. http://dx.doi.org/10.1016/S1382-
Marnett LJ. 1999. Lipid peroxidation-DNA damage by malondialdehyde. Mutation Research 424, 83-95. doi:10.1016/S0027-5107(99)00010-X
Mashkoor J, Khan A, Khan MZ, Hussain I. 2016. Chromium toxicity and oxidative stress in broiler chicks and its amelioration with vitamin E and bentonite. International Journal of Agriculture and Biology 18, 1103-1108.
Mateo R, Beyer WN, Spann JW, Hoffman DJ, Ramis A. 2003. Relationship between oxidative stress, pathology, and behavioral signs of lead poisoning in mallards. Journal of Toxicology and Environmental Health Part A 66, 1371-1389.
Mateo R, Hoffman DJ. 2001. Differences in oxidative stress between young Canada geese and mallards exposed to lead-contaminated sediment. Journal Toxicology and Environmental Health Part A 64, 531-545.
Molina-Jijon E, Tapia E, Zazueta C, El Hafidi M, Zatarain-Barrón ZL, Hernández-Pando R, Medina-Campos ON, Zarco-Márquez G, Torres I, Chaverri JP. 2011. Curcumin prevents Cr (VI)-induced renal oxidant damage by a mitochondrial pathway. Free Radical Biology and Medicine 51, 1543-1557.
National Research Council (NRC). 1994. Nutrient requirements of poultry. 9th Revised Edition, National Academy Press, Washington, DC.
Placer AZ, Linda LC, Johnson B. 1966. Estimation of product of lipid peroxidation (malonyldialdehyde) in biochemical systems. Analytical Biochemistry 16, 359-364.
Reglero MM, Taggart MA, Monsalve-González L, Mateo R. 2009. Heavy metal exposure in large game from a lead mining area: effects on oxidative stress and fatty acid composition in liver. Environmental Pollution 157, 1388-1395.
Shaheen T, Akhtar T. 2012. Assessment of chromium toxicity in Cyprinus carpio through hematological and biochemical blood markers. Turkish Journal of Zoology 36, 682-690.
Surai PF. 2016. Antioxidant systems in poultry biology: superoxide dismutase. Journal of Animal Research and Nutrition 1, 8.
Valavanidis A,Vlahogianni T, Dassenakis M, Scoullos M. 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety 64, 178-189.
Valko M, Morris H, Cronin MT. 2005. Metals toxicity and oxidation stress. Current Medicinal Chemistry 12, 1161-1208.
Weydert CJ, Cullen JJ. 2010. Measurement of superoxide dismutase, catalase, and glutathione peroxidase in cultured cells and tissue. Nature Protocols 5, 51-66.
Wu B, Cui H, Peng X, Fang J, Zuo Z, Deng J, Huang J. 2013. Investigation of the serum oxidative stress in broilers fed on diets supplemented with nickel chloride. Health 5, 454-459.
Anwar Ul Haq, Zahed Mahmood*, Muhammad Ali, Abdul Ghaffar, Muhammad Yameen, 2019. Effects of raw rock phosphate rich in heavy metals on oxidative stress markers in layers. Int. J. Biosci., 14(6), 368-373.
Copyright © 2019 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.