Seroprevalence and comparison of three different screening tests for detection of human immunodeficiency virus infection in Peshawar, Pakistan
Paper Details
Seroprevalence and comparison of three different screening tests for detection of human immunodeficiency virus infection in Peshawar, Pakistan
Abstract
Human immunodeficiency virus (HIV) concedes as a significant public health issue in the world from decades, which is growing speedily in number. Although, there seems to be insufficient data regarding HIV infection seroprevalence in general population of Peshawar, Pakistan. The objectives of the current study was to determine the seroprevalence of HIV infection in the general population of Peshawar, Pakistan and to compare the performance of three different screening methods for detection of HIV infection. A total of 5370 blood samples were screened for anti-HIV antibodies and HIV-RNA by using immunochromatographic test (ICT), enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR) at Antiretroviral Therapy (ART) centre in Hayatabad Medical Complex (HMC), Peshawar. Out of 5370 blood samples, 756 (14.07%) were positive by ICT, 639 (11.89%) by ELISA and 606 (11.28%) by RT-PCR. The active HIV infection was high in males, i.e. 468 (13.61%) than in females 138 (7.14%). It was observed that infection was highest in the age group of 21–30 years that was about 237 (16.80%), whereas no sign of infection was observed for the age group of 01-10 years. However, the married population had an HIV seroprevalence of 423 (13.83%) while singles had 183 (7.91%) respectively. ELISA should be preferred for anti-HIV antibodies detection over ICT and RT-PCR should be preferred over ELISA for HIV-RNA detection. Moreover, the provision of treatment and screening facilities against this virus should be assured in medical care units of rural areas to prevent this infection.
Adil MS, Muazzam M, Imam H, Suqrat H. 2014. HIV INFECTION; PREVALENCE AMONG INTRAVENOUS DRUG USERS. Professional Medical Journal 21(5), 975-979.
Ahmad FA, Khan AA. 2007. Knowledge of HIV/AIDS and Other Sexually Transmitted Infections (Pakistan Demographic and Health Survey 2006-7). Macro International 13, 155-166.
Akhtar H, Badshah Y, Akhtar S, Kanwal N, Akhtar MN, Qadri I.2012. Prevalence of human immunodeficiency virus infection among transgender men in Rawalpindi (Pakistan). Virology Journal 9, 1-9. https://doi.org/10.1186/1743-422X-9-229
Altaf A, Pasha S, Vermund SH, Shah SA. 2016. A second major HIV outbreak in Larkana, Pakistan. Journal of Pakistan Medical Association 66(12), 1510-1511.
Altaf A, Saleem N, Abbas S, Muzaffar R.2009. High prevalence of HIV infection among injection drug users (IDUs) in Hyderabad and Sukkur, Pakistan. Journal of Pakistan Medical Association 59(3), 136-140.
Alter MJ. 2006. Epidemiology of viral hepatitis and HIV co-infection. Journal of Hepatology 44, 6-9. https://doi.org/10.1016/j.jhep.2005.11.004
Azuonwu O, Ihua A, Wokem GH. 2017. Prevalence of Human Immunodeficiency Virus (HIV) antibody among subjects in Ogba/Egbema/Ndoni Local Government Area (LGA) of Rivers State of Nigeria. Translation Biomedicine 8(3), 1-5. https://doi.org/10.21767/2172-0479.100118
Bhurgri Y. 2006. HIV/AIDS in Pakistan. Journal of Pakistan Medical Association 56, 3-4.
Burnett RJ, Francois G, Kew MC, Leroux‐Roels G, Meheus A, Hoosen AA, Mphahlele MJ. 2005. Hepatitis B virus and human immunodeficiency virus co‐infection in sub-Saharan Africa: a call for further investigation. Liver International 25(2), 201-213. https://doi.org/10.1111/j.1478-3231.2005.01054.x
Dieffenbach CW, Fauci AS. 2011. Thirty years of HIV and AIDS: future challenges and opportunities. Annals of Internal Medicine 154(11), 766-771. https://doi.org/10.7326/0003-4819-154-11-201106070-00345
Jenness SM, Hagan H, Liu KL, Wendel T, Murrill CS. 2011. Continuing HIV risk in New York City injection drug users: the association of syringe source and syringe sharing. Substance Use and Misuse 46(2-3), 192-200. https://doi.org/10.3109/10826084.2011.521467
Junaid M, Siddique AN, Khan MT, Alam I, Waqas M, Saba, Hameed S, Muhammad W. 2017. Detection and prevalence of hepatitis B, C and HIV viral infections among hemophilia patients in Peshawar, Pakistan. Journal of Entomology and Zoology Studies 5(2), 180-184.
Khattak MU, Munir I, Nasim Z, Iqbal A, Ali I, Ahmad MA, Bangash JA, Kazi MSK. 2014. Incidence of HIV/AIDS in Healthy blood donors of Khyber Pakhtunkhwa, Pakistan. International Journal of Pure Applied Sciences and Technology 22(2), 18-24.
Lee LM, McKenna MT, Janssen RS. 2003. Classification of transmission risk in the national HIV/AIDS surveillance system. Public Health Reports 118(5), 400-407. https://doi.org/10.1016/S0033-3549(04)50271-6
Maartens G, Celum C, Lewin SR. 2014. HIV infection: epidemiology, pathogenesis, treatment, and prevention. The Lancet 384(9939), 258-271. https://doi.org/10.1016/S0140-6736(14)60164-1
Maimaiti R, Andersson R. 2008. Awareness and attitudes about HIV among pregnant women in Aksu, Northwest China. Open AIDS Journal 2, 72-77. https://doi.org/10.2174/1874613600802010072
Mylonakis E, Paliou M, Lally M, Flanigan TP, Rich JD. 2000. Laboratory testing for infection with the human immunodeficiency virus: established and novel approaches. American Journal of Medicine 109(7), 568-576. https://doi.org/10.1016/S0002-9343(00)00583-0
Nafees M, Qasim A, Jafferi G, Anwar MS, Muazzam M. 2011. HIV Infection, HIV/HCV and HIV/HBV co-infections among Jail Inmates of Lahore. Pakistan Journal of Medical Sciences 27(4), 837-841.
Rothenberg RB, Scarlett M, Carlos DR, Reznik D, O’Daniels C.1998. Oral transmission of HIV. AIDS 12(16), 2095-2105. https://doi.org/10.1097/00002030-199816000-00004
Shahid S, Majeed MF, Awaan AB, Mirza H, Sarfraz N, Veronese V. 2016. Expanding access to HIV testing and counseling and exploring vulnerabilities among spouses of HIV-positive men who inject drugs in Pakistan. Current Opinionin HIV and AIDS 1, 6-12. https://doi.org/10.1097/COH.0000000000000263
Talarok P, Talaro A. 2002. The RNA viruses of Medical Importance. Foundation in Microbiology. 4th ed. MilGraw Hill, New York, 750-789.
UNICEF, UNAIDS, WHO. 2008. Epidemiological Fact Sheet on HIV and AIDS. Core data on epidemiology and response. Nigeria, WHO, Geneva 1-25.
Valadas E, Fran AL, Sousa S, Antunes F. 2009. 20 years of HIV-2 infection in Portugal: trends and changes in epidemiology. Clinical Infectious Diseases 48(8), 1166-1167. https://doi.org/10.1086/597501
Zareen S, Rehman FU, Zareen H, Rehman HU, Zakir M, Khan A, Rehman AU, Javid K, Ziafat R. 2016. Burden of HIV infection among rural and urban population of district Swabi, Khyber Pakhtunkhwa, Pakistan. Journal of Entomology and Zoology Studies 4(4), 1084-1088.
Saba, Iqbal Hussain, Nasir Ali, Muhammad Junaid, Rahim Dost Khan, Safia Hameed, Muhammad Waqas, Jawaria Shah, 2019. Seroprevalence and comparison of three different screening tests for detection of human immunodeficiency virus infection in Peshawar, Pakistan. Int. J. Biosci., 15(3), 169-174.
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