A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes

Paper Details

Research Paper 01/12/2015
Views (652)
current_issue_feature_image
publication_file

A modified genomic DNA extraction method from leaves of sunflower for PCR based analyzes

Hossein Zeinalzadehtabrizi, Arash Hosseinpour, Murat Aydin, Kamil Haliloglu
J. Biodiv. & Environ. Sci. 7(6), 222-225, December 2015.
Copyright Statement: Copyright 2015; The Author(s).
License: CC BY-NC 4.0

Abstract

Sunflower genomic DNA extraction for PCR (Polymerase Chain Reaction) analyses and similar molecular technics is a challenging issue because of high concentrations of secondary metabolites, i.e. polyphenolics, polysaccharides, tannins and other contaminants. Our modified (Cetyl Trimethyl Ammonium Bromide) CTAB protocol can be performed approximately in two hours and suitable for PCR analyses. It needed small amounts of plant leaves and good quality of sunflower genomic DNA can be isolated. Quantity and purity of extracted DNA were checked by spectrophotometry and agarose gel electrophoresis. This modified method takes less time, reliable and gives good quality of DNA even without using RNase can be used in various marker systems such as (Single Sequence Repeat) SSR and (Random Amplified Polymorphic DNA) RAPD.

Amani J, Kazemi R, Abbasi AR, Salmanian AH. 2011. A simple and rapid leaf genomic DNA extraction method for polymerase chain reaction analysis. Iran J Biotechnol. 9, 69-71.

Encheva J, Köhler H, Christov M, Friedt W. 2005. Intergeneric hybrids between cultivated sunflower (Helianthus annuus L.) and Verbesina helianthoides (genus Verbesina): RAPD analysis. Heila 28(42), 37-44.

Horne EC, Kumpatla SP, Patterson KA, Gupta M, Thompson SA. 2004. Improved high-throughput sunflower and cotton genomic DNA extraction and PCR fidelity. Plant Molecular Biology Reporter 22(1), 83-84.

Iqbal M, Sadaqat H, Khan I. 2008. Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis. Genet. Mol. Res. 7(Suppl 4), 1408-1413.

Isaacs MS, Manivannan N, Muralidharan V. 2003. Genetic diversity analysis using RAPD marker in inbred lines of sunflower Helianthus annuus L. Helia 26(39), 59-65.

Li J, Yang J, Chen D, Zhang X, Tang Z. 2007. An optimized mini-preparation method to obtain high-quality genomic DNA from mature leaves of sunflower. Genet. Mol. Res 6(4), 1064-1071.

Zhizhong X. 2008. DNA extraction from oil sunflower seeds and the optimization of its RAPD reaction system. Journal of Anhui Agricultural Sciences 36(6), 2259.

Related Articles

Phytochemical analysis and antioxidant activity of ethanolic leaves extract of Psidium guajava

G. Saranya, K. Durgadevi, V. Ramamurthy, J. Biodiv. & Environ. Sci. 27(3), 57-63, September 2025.

Physicochemical and phytochemical analysis of Glycyrrhiza glabra root extract

J. Ramalakshmi, P. Vinodhiniand, V. Ramamurthy, J. Biodiv. & Environ. Sci. 27(3), 50-56, September 2025.

The effects of climate change on built structures in Benin-city, Edo state, Nigeria

A. E. Ilenre, Y. H. Tashok, E. N. Uhumuavbi, J. Biodiv. & Environ. Sci. 27(3), 39-49, September 2025.

A review of the ecological impacts of water pollution on lakes: From diatom assemblages to migratory bird population declines

Arunpandiyan Murugesan, Roshy Ann Mathews, S. Rajakumar, Prashanthi Devi Marimuthu, J. Biodiv. & Environ. Sci. 27(3), 23-38, September 2025.

Agroforestry in woody-encroached Sub-Saharan savannas: Transforming ecological challenges into sustainable opportunities

Yao Anicet Gervais Kouamé, Pabo Quévin Oula, Kouamé Fulgence Koffi, Ollo Sib, Adama Bakayoko, Karidia Traoré, J. Biodiv. & Environ. Sci. 27(3), 10-22, September 2025.

Extreme rainfall variability and trends in the district of Ouedeme, municipality of Glazoue (Benin)

Koumassi Dègla Hervé, J. Biodiv. & Environ. Sci. 27(3), 1-9, September 2025.

Heterosis breeding, general and specific combining ability and stability studies in pearl millet: Current trends

Ram Avtar, Krishan Pal, Kavita Rani, Rohit Kumar Tiwari, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 117-124, August 2025.

Combining ability, heterosis and stability for yield and fibre quality traits in cotton: Breeding approaches and future prospects

Rohit Kumar Tiwari, Krishan Pal, R. P. Saharan, Ram Avtar, Mahendra Kumar Yadav, J. Biodiv. & Environ. Sci. 27(2), 109-116, August 2025.