A phylogenetic analysis of Hyoscyamus L. (Solanaceae) species from Iran based on ITS and trnL-F sequence data

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A phylogenetic analysis of Hyoscyamus L. (Solanaceae) species from Iran based on ITS and trnL-F sequence data

Shadi Hajrasouliha, Ali Asghar Massoumi, Taher Nejad Sattari, Seyed Mohammad Mahdi Hamdi, Iraj Mehregan
J. Bio. Env. Sci.5( 1), 647-654, July 2014.
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A phylogeny of Hyoscyamus L. (Solanaceae) that represents two different subgenera (i.e Hyoscyamus subgen. Dendrotrichon SchÖnbeck-Temesy and Hyoscyamus subgen. Hyoscyamus) based on ITS and trnL-F sequence data were studied. In this study five species of Hyoscyamus were Iran endemic. ITS and trnL-F sequence data for 18 species of this genus from Iran were studied. In addition we used 8 ITS sequence data and 13 trnL-F sequence data from genbank for another taxa in Solanaceae family. For phylogenetic analysis were used Bayesian and Maximum parsimony methods. Parsimony analyses of the ITS and trnL-F regions formed a dendrogram with strong bootstrap (ca.≥70%) that showed Hyoscyamus is a monophyletic group. In this study four synonymous species (H.insanus Stocks, H.rosularis SchÖnbeck-Temesy, H.orthocarpus SchÖnbeck-Temesy, H.nutans SchÖnbeck-Temesy) were stand in one clade and two similisar species H.bornmulleri Khatamsaz and H.tenuicaulis SchÖnbeck-Temesy constituted one clade. Also two species H.senecionis Willd and H.pusillus L. were formed one clade in trnL-F and combined analyses


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