A review on antimicrobial efficacy, toxicity and phytochemicals of selected medicinal plants used in Africa against pathogenic microorganisms

Paper Details

Review Paper 01/03/2017
Views (344) Download (11)
current_issue_feature_image
publication_file

A review on antimicrobial efficacy, toxicity and phytochemicals of selected medicinal plants used in Africa against pathogenic microorganisms

Mhuji Kilonzo, Musa Chacha, Patrick A. Ndakidemi
Int. J. Biosci.10( 3), 232-250, March 2017.
Certificate: IJB 2017 [Generate Certificate]

Abstract

Medicinal plants have been widely used as antimicrobial agents to treat a variety of infectious diseases such as pneumonia, stomach, urinary tract infection, gonorrhea and dysentery. According to an estimate, 80% of the antimicrobial agents which are widely used worldwide for the management of infectious diseases are derived from native plants. A variety of native plants in Africa have shown promise to treat different infectious diseases and some of them possess broad spectrum antimicrobial activities. This article therefore describes potential antimicrobial efficacy and safety of medicinal plants namely Mystroxylon aethiopicum, Lonchocarpus capassa, Albizia anthelmentica and Myrica salicifolia which are used by ethnic groups in Africa for the management of infectious diseases. Since these plants possess broad spectrum antimicrobial properties, the article suggests future in vivo studies which will ultimately lead to the development plant based pharmaceutical formulations.

VIEWS 16

Acar JF. 1997. Consequences of bacterial resistance to antibiotics in medical practice. Clinical Infectious Diseases-Supplements 24(1), 17-18.

Adelowo F, Oladeji O. 2016. A review on Tridax procumbens: a weed with immense phytochemical and pharmacological activities. Communication in Plant Sciences 7, 1-9.

Aganga AA, Mosase KW. 2001. Tannin content, nutritive value and dry matter digestibility of Lonchocarpus capassa, Zizyphu smucronata, Sclerocarya birrea, Kirkia acuminata and Rhuslancea seeds. Animal Feed Sciences and Technology 91(1), 107-113.

Ahmad K, Mehdi R, Motahare A, Mitra M. 2013. Molecular detection of bla TEM and bla SHV genes among clinical isolates of Escherichia coli from Kashan, Iran. African Journal of Microbiology Research 7(9), 751-754.

Akomo EF, Atteke C, Karou S, Zongo C, Obame LC, Savadogo A, Atteke C, Traore AS. 2009. In vitro antiplasmodial and antibacterial activities of Canthium multiflorum Schum and Thonn (Rubiacea) extracts. Pakistan Journal of Biological Sciences 12(12), 919-923.

Alves MJ, Ferreira IC, Dias J, Teixeira V, Martins A, Pintado M. 2013. A review on antifungal activity of mushroom (Basidiomycetes) extracts and isolated compounds. Current Topics in Medicinal Chemistry 13(21), 2648-2659.

Aniagu SO, Wily FC, Akumka DD, Ajoku GA, Dzarma S, Izebe KS. 2005. Toxicity study in rats fed nature cure bitters. African Journal of Biotechnology 4(1), 72-78.

Arif T, Bhosale JD, Kumar N, Mandal TK, Bendre RS, Lave kar GS. 2009. Natural products-antifungal agents derived from plants. Journal of Asian Natural Product Research 11(7), 621-638.

Arora S, Kumar D. 2013. Phytochemical, antimicrobial and antioxidant activities of methanol extract of leaves and flowers of Ipomoea cairica. International Journal of Pharmacy and Pharmaceutical Sciences 5(1), 198-202.

Ashafa AO, Orekoya LO, Yakubu MT. 2012.Toxicity profile of ethanolic extract of Azadirachta indica stem bark in male Wistarrats. Asian Pacific Journal of Tropical Biomedicine 2(10), 813-817.

Athanasiadou S, Kyriazakis I, Jackson F, Coop RL. 2001. Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: In vitro and in vivo studies. Veterinary Parasitology 99(3), 205-219.

Baikar S, Malpathak N. 2010. Secondary metabolites as DNA topoisomerase inhibitors: a new era towards designing of anticancer drugs. Pharmacognosy Review 4(7), 12-26.

Baki MA, Khan A, Al-Bari MA, Mosaddik A, Sadik G, Mondal, KA. 2007. Sub-acute toxicological studies of Pongamol isolated from Pongamia pinnata. Research Journal of Medical Sciences 2, 53-57.

Balaj K, Kilimozhi D, Parthasarathy V. 2014. GC-MS analysis of various extracts of Clerodendrum phlomidis leaf. International Journal of Pharmacy and Pharmaceutical Sciences 6(1), 226-232.

Balouiri M, Sadiki M, Ibnsouda SK. 2016. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis 6(2), 71-79.

Belguet A. 2010. Evaluation of toxicity in mice and rats and antioxidant activities of Ruta montana leaves extracts. PhD thesis, University of Ferhat Abbas Sétif 1, Algeria, 18-37.

Bennett RN, Walls grove RM. 1994. Secondary metabolites in plant defense mechanisms. New Phytologist 127(4), 617-633.

Bhalodia NR, Shukla VJ. 2011. Antibacterial and antifungal activities from leaf extracts of Cassia fistula l. An ethno medicinal plant. Journal of Advanced Pharmaceutical Technology and Research 2(2), 104-109.

Blomberg B. 2007. Antimicrobial resistance in bacterial infections in urban and rural Tanzania. PhD thesis, University of Bergen, Norway 22-35.

Bockstael K, Aerschot A. 2009. Antimicrobial resistance in bacteria. Open Medicine 4(2), 141-155.

Boer HJ, Koola A, Broberg A., William R, Mziray WR, Hedberg I, Levenfors JJ. 2005. Antifungal and antibacterial activity of some herbal remedies from Tanzania. Journal of Ethno pharmacology 96(3), 461-469.

Borde V, Sonwane B, Sontakke V, Somwanshi B. 2014. Isolation and purification of alkaloids from medicinal plants by HPLC. International Journal of Current Microbiology and Applied Sciences 3(1), 414-423.

Botha CJ, Penrith ML. 2008. Poisonous plants of veterinary and human importance in southern Africa. Journal of Ethnopharmacology 119, 549-558.

Bruneton J. 1995. Pharmacognosy, phytochemistry and medicinal plants. Lavoisier Publishing, Paris 22-27.

Burkil HM. 2004.The useful plants of west tropical Africa. Royal Botanic Gardens, Kew 12-15.

Bush K, Fisher JF. 2011. Epidemiological expansion, structural studies and clinical challenges of new beta-lactamases from Gram-negative bacteria. Annual Review Microbiology 65, 455-478.

Bush K, Jacoby GA, Medeiros AA. 1995. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrobial Agents and Chemotherapy 39(6), 1211-1233.

Bussmann RW, Malca-Garcìa G, Glenn A, Sharon D, Nilsen B, Parris B, Dubose D, Ruiz D, Saleda J, Martinez M, Carillo L. 2011. Toxicity of medicinal plants used in traditional medicine in Northern Peru. Journal of Ethno pharmacology 137(1), 121-140.

Carballo JL, Hernández-Inda ZL, Pérez P, García-Grávalos MD. 2002. A comparison between two brines shrimp assays to detect in vitro cytotoxicity in marine natural products. BMC Biotechnology 2, 17.

Carson CF, Hammer KA.2010. Chemistry and bioactivity of essential oils. In: lipids and essential oils as antimicrobial agents, Thormar H. (Eds). John Wiley & Sons, New York, USA 35-47.

Carvalho AA. 2008. Management of fungal infections: diagnosis and human genetic susceptibility. PhD thesis, University of Minho, Portugal 22-34.

Cekic SD, Cetinkaya A, Avan AN, Apak R. 2013. Correlation of total antioxidant capacity with reactive oxygen species (ROS) consumption measured by oxidative conversion. Journal of Agricultural and Food Chemistry 61(22), 5260-5270.

Chandrika M, Chellaram C.2015. Antibacterial properties of leaf extracts of Borreria hispida from Tamilandu regions, India. Der Pharma Chemica 7(7), 389-394.

Compean KL, Ynalvez RA. 2014. Antimicrobial activity of plant secondary metabolites: A review. Research Journal of Medicinal Plants 8(5), 204-213.

Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiology Review 12(4), 564-582.

Croteau R, Kutchan TM, Lewis NG. 2000. Natural products (Secondary Metabolites). Biochemistry and Molecular Biology Plants 24, 1250-1319.

Dai J, Mumper RJ. 2010. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 15(10), 7313-7352.

Dembitsky VM. 2005. Astonishing diversity of natural surfactants: 6. Biologically active marine and terrestrial alkaloid glycosides. Journal of Lipids 40(11), 1081-1105.

Desta B. 1995. Ethiopian traditional herbal drugs. Part I: Studies on the toxicity and therapeutic activity of local Taenicidal medications. Journal of Ethno pharmacology 45(1), 27-33.

Devika R. Koilpillai J. 2012. An overview on plant secondary metabolites: Its medicinal importance. Journal of Pharmacy Research 5(2), 984-986.

Dharmananda S.2003. Gallnuts and the uses of tannins in Chinese medicine. PhD thesis, Institute for Traditional Medicine, Portland, Oregon, 34-45.

Dini I, Tenore GC, Din A. 2002. Oleananesaponins in “kancolla”, a sweet variety of Chenopodium quinoa. Journal of Natural Products 65, 1023-1026.

Doughari JH, Ndakidemi PA, Human IS, Benadé S. 2009. Shiga toxins (verocytotoxins).African Journal of Microbiology Research 3(11), 681-693.

Duraipandiyan V, Ignacimuthu S. 2011. Antifungal activity of traditional medicinal plants from Tamil Nadu, India. Asian Pacific Journal of Tropical Biomedicine 1(2), 204-215.

Elkadry A. 2016. Transmission rate in partial differential equation in epidemic MSc thesis, Marshall University, United States 22-30.

Elnour EA, Abdmageed MA, Shyoub ME, Mohammed RR. 2013. Evaluation of toxicity of some plants having traditional uses in Sudan on brine shrimp. Global Journal of Traditional Medicinal Systems 2(1),19-23.

Emma NQ, Antonio RS, Marta AV. 2001. Screening antifungal activities of selected medicinal plants. Journal of Ethno pharmacology 74(1), 89-96.

Estrada A, Katselis GS, Laarveld B, Barl B. 2000. Isolation and evaluation of immunological adjuvant activities of saponins from Polygala senega L. Comparative Immunology. Microbiology Infectious Diseases 23, 27-43.

Fluit AC, Visser MR, Schmitz FJ. 2001. Molecular detection of antimicrobial resistance. Clinical Microbiology Review 14(4), 836-71.

Furiga A, Lonvaud FA, Badet C.2009. In vitro study of antioxidant capacity and antibacterial activity on oral anaerobes of a grape seed extract. Journal of Food Chemistry 113(4), 1037-1040.

Ghani A. 2013. Herbal medicines: Present status, future prospects. Pharma World.6, 59-62.

Ghosh A, Das BK, Roy A, Mandal B, Chandra G. 2008. Antibacterial activity of some medicinal plant extracts. Journal of Natural Medicines 62(2), 259-262.

Goldman P. 2001. Herbal medicines today and the roots of modern pharmacology. Annals of Internal Medicines 135(8), 594-600.

Görgen H. 2001. The history of health care in Tanzania. Organized by German agency for technical cooperation and National museum of Tanzania. Publisher: Dar es Salaam, GTZ: The Museum, Tanzania 45-55.

Grade JT, Arble BL, Weladji RB, Van Damme P. 2008. Anthelmintic efficacy and dose determination of Albizia anthelmintica against gastrointestinal nematodes in naturally infected Ugandan sheep. Vetenary Parasitology 157(3), 267-274.

Hamza OJ, Beukel CJ, Matee MI, Moshi MJ, Mikx FH, Selemani HO, Mbwambo ZH, Van der Ven AJ, Verweij PE. 2006. Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections. Journal of Ethno pharmacology 108(1), 124-132.

Harborne JB, Walton NJ, Brown DE. 1996. Classes and functions of secondary products in chemicals from plants, perspectives on secondary plant products. Imperial College Press 1-25.

Harborne JB. 2003. Higher plant-lower plants interactions: phytoalexins and phytotoxins from ecological biochemistry. 2nd edition: Elsevier Academic Press 22-30.

Harvey A. 2000. Strategies for discovering drugs from previously unexplored natural products. Drug Discovery Today 5(7), 294-300.

Hemingway RW, Laks PE. 2012. Plant polyphenols: synthesis, properties, significance. Springer Science & Business Media 43-60.

Joseph C, Togawa Y, Shindo N. 2013. Bacterial and viral infections associated with influenza. Influenza and Other Respiratory Viruses 7(2), 105-113.

Kaigongi MM. 2014. Antimicrobial activity, toxicity and phytochemical analysis of four medicinal plants traditionally used in Msambweni District, Kenya. PhD thesis, University of Nairobi, Kenya 18-34.

Kaur R, Arora S. 2015. Alkaloids-important therapeutic secondary metabolites of plant origin. Journal of Critical Review 2(3), 3-8.

Kilonzo M, Ndakidemi P, Chacha M. 2016a. In vitro antibacterial activity of selected Tanzania medicinal plants. Herbal Medicines 2, 2.

Kilonzo M, Ndakidemi PA, Chacha M. 2016b. In vitro antifungal and cytotoxicity activities of selected Tanzanian medicinal plants. Tropical Journal of Pharmaceutical Research 15(10), 2121-2130.

Kishore N, Mishra BB, Tiwari VK, Tripathi V. 2011. A review on natural products with mosquito sidal potentials. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry 2, 335-365.

Klepser ME. 2006. Candida resistance and its clinical relevance. Pharmacotherapy: Journal of Human Pharmacology and. Drug Therapy 26(6), 68S-75S.

Kokwaro JO. 1993. Medicinal plants of East Africa. 2nd edition: Published and printed by Kenya literature bureau, Nairobi Kenya 23-30.

Kumar S, Mishra A, Pandey AK. 2013. Antioxidant mediated protective effect of Partheniumhysterophorus against oxidative damage using in vitro models. BMC Complementary and Alternative Medicines 13(1), 2-9.

Kumar S, Varela MF. 2013. Molecular mechanisms of bacterial resistance to antimicrobial agents. Chemotherapy 14(18), 522-534.

Lahlou M. 2013. The success of natural products in drug discovery. Pharmacology and Pharmacy 4(3A), 17-31.

Lai PK, Roy J. (2004. Antimicrobial and chemo preventive properties of herbs and spices. Current medicinal chemistry 11(11), 1451-1460.

Levy SB. 1998. The challenge of antibiotic resistance. Scientific American Journal 278(3), 46-53.

Loomis TA, Hayes AW. 1996. Loomis’s essentials of toxicology. 4th edition California, Academic Press 22-35.

Maara TN. 2014. A review of traditional knowledge, usage and status of the medicinal trees amongst the Tugens of Eldama ravine and Esageri divisions, Koibatek County, Kenya. Journal of Health Medicines and Nursing 5, 113-128.

Margaret FR, Michael W. 1998. Alkaloids, biochemistry, ecology and medicinal application. 1st edition: Plenum Press: New York 120- 130.

Martins N, Barros L, Henriques M, Silv, S, Ferreira IC. 2015. Activity of phenolic compounds from plant origin against Candida species. Industrial Crops and Products 74(15), 648-670.

Matthaiou D, Christodoulopoulou T, Dimopoulos G. 2015. How to treat fungal infections in ICU patients. BMC infectious Diseases 15(1), 1-8.

Mbuya LP, Msanga HP, Ruffo CK, Birnie A, Tengnäs B. 1994. Useful trees and shrubs for Tanzania. Identification, propagation and management for agricultural and pastoral communities.6th edition. Regional soil conservation unit, Swedish international development authority 96-102.

Mengistu A. 2015. Evaluation of acute and sub-chronic toxicity of aqueous leaves extracts of Maytenus gracilipes celastraciae (kombolcha) on some blood parameters and histopathology of liver and kidney in Swiss albino mice. PhD thesis, Addis Ababa University, Ethiopia 22-30.

Mohamed TK, Nassar MI, Gaara AH, El-Kashak WA, Brouard I, El-Toumy SA. 2013. Secondary metabolites and bioactivities of Albizia anthelmintica. Pharmacognosy Research 5(2), 80-85.

Morales-González JA. 2013. Oxidative stress and chronic degenerative diseases. A role for antioxidants. 1st edition: Published and printed by Intech Press, Rio de Janeiro, Brazil 20-26.

Moreira DD, Teixeira SS, Monteiro MH, De-Oliveira AC, Paumgartten FJ. 2014. Traditional use and safety of herbal medicines. Revista Brasileira de Farmacognosia 24(2), 248-257.

Moshi MJ, Mbwambo ZH, Nondo RS, Masimba PJ, Kamuhabwa A, Kapingu MC, Thomas P. 2006. Evaluation of ethno medical claims and brine shrimp toxicity of some plants used in Tanzania as traditional medicines. African Journal of Complementary and Alternative Medicines 3(3), 48-58.

Moshi MJ, Otieno DF, Mbabazi PK, Weisheit A. 2009.The ethnomedicine of the Haya people of Bugabo ward, Kagera Region, North Western Tanzania. Journal of Ethnobiology and Ethnomedicines 5(24), 1-5.

Munyendo WL, Orwa JA, Rukunga GM, Bii CC. 2011. Bacteriostatic and bactericidal activities of Aspilia mossambicensis, Ocimum gratissimum and Toddalia asiatica extracts on selected pathogenic bacteria. Research Journal of Medicinal Plants 5, 717-727.

Murtaza G, Mukhtar M, Sarfraz A. 2015. A review: Antifungal potentials of medicinal plants. Journal of Bioresource Management 2(2), 4-8.

Mutembei J, Njonge K, Kareru P, Kutima H, Karanja J. 2014. Antimicrobial and phytochemical evaluation of selected medicinal plants in Meru community of Kenya. Proceedings: Basic and Applied Sciences for Advancement of Research and Industrialization. 8th Jomo Kenyatta University of Agriculture and Technology (JKUAT) Scientific and Technological Conference.

Naidu JR, Ismail R, Sasidharan S. 2014. Acute oral toxicity and brine shrimp lethality of methanol extract of Mentha spicata L (Lamiaceae). Tropical Journal of Pharmaceutical Research 13(1), 101-107.

Nasri H, Shirzad H. 2013. Toxicity and safety of medicinal plants. Journal of Herbal Medicines and Plarmacology 2(2), 21-22.

Ndukui JG, Mugisha K, Owiny D, Waako P. 2014. Effects of crude aqueous leaf extracts of Citropsis articulata and Mystroxylon aethiopicum on sex hormone levels in male albino rats. International Journal of Pharmaceutical Science Invention 3(1), 5-17.

Njung’e K, Mariuki G, Mwangi JW, Kuria KA. 2002. Analgesic and antipyretic effects of Myrica salicifolia (Myricaceae). Phytotherapy Research 16(1), 73-74.

Ojo OA, Oloyede OI, Olarewaju OI, Ojo AB, Ajiboye BO, Onikanni SA. 2013. Toxicity studies of the crude aqueous leaves extracts of Ocimum gratissimum in albino rats. Journal of Environmental Sciences, Toxicology and Food Technology 6(4), 34-39.

Okeke IN, Laxminarayan R, Bhutta ZA, Duse AG, Jenkins P, O’Brien TF. 2005. Antimicrobial resistance in developing countries. Part I: Recent trends and current status. Lancet Infectious Diseases 5(8), 481-493.

Oksman-caldentey KM, Barz W. 2002. Plant biotechnology and transgenic plants. 2nd edition: Published and printed by CRC Press, Taylor & Francis Group, FL, United State 22-33.

Onyango CA, Gakuya LW, Mathooko FM, Maina JM, Nyaberi MO, Makobe M, Mwaura F. 2014. Phytochemical studies on herbal plants commonly used for processing and preserving meat and milk. Journal of Applied Biosciences 73(1), 5942-5948.

Organization for Economic Cooperation and Development (OECD). 2001. Guidelines for testing of chemicals. Health effects – Test No. 425. Acute oral toxicity: Up-and-Down procedures.

Orwa C, Mutua A, Kindt R, Jamnadass R, Simon A. 2015. Agroforestry database: A tree reference and selection guide handbook. World Agroforestry Centre, Kenya.

Pandey AK, Kumar S.2012. Antioxidant, lipo-protective and antibacterial activities of phytoconstituents present in Solanum xanthocarpum root. International Review of Biophysical Chemistry 3(3), 42-47.

Pandey AK, Shashank K. 2013. Perspective on plant products as antimicrobials agents: A review. Journal of Pharmacology 4(7), 469-480.

Petersen PE, Bourgeois D, Ogawa HE, Ndiaye C. 2005. The global burden of oral diseases and risks to oral health. Bull World Health Organization 83(9), 661-669.

Pinto BM. 1999. Comprehensive natural products chemistry, 3rd edition. Carbohydrates and their derivatives including tannins, cellulose, and related lignins: Amsterdam, Elsevier 67-80.

Politi FA, de Souza-Moreira TM, Rodrigues ER, de Queiroz GM, Figueira GM, Januário AH, Berenger JM, Socolovschi C, Parola P, Pietro RC. 2013. Chemical characterization and acaricide potential of essential oil from aerial parts of Tagetes patula L. (Asteraceae) against engorged adult females of Rhipicephalus sanguineus. Parasitology Research 112(6), 2261-2268.

Portillo A, Vila R, Freixa B, Adzet T, Cañigueral S. 2001. Antifungal activity of Paraguayan plants used in traditional medicine. Journal of Ethno pharmacology 76(1), 93-98.

Prakash S, Shelke AU. 2014. Role of Triphala in dentistry. Journal of Indian Society Period ontology 18(2), 132-138.

Rachana S, Tarun A, Rinki R, Neha A, Meghna, R. 2012. Comparative analysis of antibacterial activity of Jatropha curcas fruit parts. Journal of Pharmacy and Biomedicine Sciences 15(15), 1-4.

Ramanan C, Ranjith PV, Mehta MJ. 2007. Future prospects of herbal medicines in India. www.siescoms.edu/images/pdf/future%20prospects%20of%20herbal%20medicines%20in%20india.pdf. [Accessed 17 November 2016].

Ramawat KG, Merillon JM. 2007. Secondary metabolites: Plants and Microbes. Science Publishers, Enfield, NH, United States 111-120.

Razia M, Rajalakshmi BS, Lavanya K, Karthiga V, Bernala W, Deboral P. 2013. GC-MS, FTIR and in vitro antibacterial activity of Abutilon indicum. International Journal of Biological and Pharmaceutical Research 4(4), 256-260.

Robbins RJ. 2003. Phenolic acids in foods: An overview of analytical methodology. Journal of Agriculture and Food Chemistry 51(10), 2866-2887.

Rodriguez-Tudela JL, Alastruey-Izquierdok A, Gagok S, Cuenca-Estrella M, León C, Miro JM, Boluda AN, Camps IR, Sole A, d Denning DW. 2015. Burden of serious fungal infections in Spain. Clinical Microbiology and Infection 21(2), 183-189.

Rosenthal N, Brown S. 2007. The mouse ascending: perspectives for human-disease models. Nature Cell Biology 9, 993-999.

Rubaka C, Ndakidemi P, Malebo P, Shahada F. 2014. Analysis of phytochemical and antibacterial activity of Carissa spinarum Linn crude extracts. European Journal of Medicinal Plants 4(8), 937-945.

Rubió L, Motilva MJ, Romero MP. 2013. Recent advances in biologically active compounds in herbs and spices: A review of the most effective antioxidant and anti-inflammatory active principles. Critical Reviews in Food Sciences and Nutrition 53(9), 943-953.

Saikia S, Nicholson MJ, Young C, Parker EJ, Scott B. 2008. The genetic basis for indole-diterpene chemical diversity in filamentous fungi. Mycology Research 112(2), 184-199.

Saleem M, Nazir M, Ali MS, Hussain H, Lee YS, Jabbar A. 2010. Antimicrobial natural products: An update on future antibiotic drug candidates. Natural Product Reports 27(2), 238-254.

SalimAA,Chin YW,Kinghorn AD. 2008. Bioactive molecules and medicinal plants: Drug discovery from plants. Springer Science & Business Media 1-6.

Savoia D. 2012. Plant-derived antimicrobial compounds: Alternatives to antibiotics. Future Microbiology 7, 979-990.

Scalbert A. 1991. Antimicrobial properties of tannins. Journal of Phytochemistry 30(12), 3875-3883.

Sharma C, Singh C, Sharma LN, Purvia R, Adlakha M. 2015. Antibiotic resistant organism: an emerging public health problem and role of Ayurveda. International Journal of Ayurveda and Pharma Research 2(1), 17-29.

Singh B, Sharma RA. 2015. Plant terpenes: defense responses, phylogenetic analysis, regulation and clinical applications. 3 Biotech 5(2), 129-151.

Sosa AJ, Byarugaba DK, Amabile-Cuevas CF, Hsueh PR, Kariuki S, Okeke IN.2010. Antimicrobial resistance in developing countries. 1st edition: Printed and Published by Springer New York, Dordrecht Heidelberg, London 11-20.

Sparg SG, Light ME, Van Staden J. 2004. Biological activities and distribution of plant saponins. Journal of Ethnopharmacology 94, 219-24.

Stephan H, Mathew HS. 2005. Antimicrobial resistance determinants and future control. Emerging Infectious Diseases 11(6), 794-801.

Summers AO. 2002. Generally overlooked fundamentals of bacterial genetics and ecology. Clinical Infectious Diseases 34(3), 85-92.

Tanwar J, Das S, Fatima Z, Hameed S. 2014. Multidrug resistance: an emerging crisis. Interdisciplinary Perspectives on Infectious diseases 2014, 1-7. DOI:10.1155/2014/541340.

Taulo S, Wetlesen A, Abrahamsen R, Mkakosy R, Kululanga G. 2008. Microbiological quality of water, associated management practices and risks at source, transport and storage points in a rural community of Lungwena, Malawi. African Journal of Microbiology Research 7(2), 131-137.

Tenover FC. 2006. Mechanisms of antimicrobial resistance in bacteria. American Journal of Medicines 119(6), S3-S10.

Thevasundari S, Rajendran A. 2012. Antifungal activity of Heterostemma tanjorense (Wight and Arn.).Asian Journal of Plant Science and Research 2(3), 330-334.

Tiwari R, Rana CS. 2015. Plant secondary metabolites: A review. International Journal of Engineering Research and General Sciences 3(5), 661-670.

Tomlinson TM, Akerele O. 1998. Medicinal plants their role in health and biodiversity. 2nd edition: Published by University of Pennsylvania, Philadelphia Press, United States, 23-36.

Vadhana P, Singh BR, Bharadwaj M, Singh SV. 2015. Emergence of herbal antimicrobial drug resistance in clinical bacterial isolates. Pharmaceutical Analytical Acta 6(10), 434.

Verónica TC, Julieth CR, Bibiana ZL, Camilo DS, Ana CA. 2011. Anti-Candida albicans activity, cytotoxicity and interaction with antifungal drugs of essential oils and extracts from aromatic and medicinal plants. Asociación Colombiana de Infectología 15(3), 160-167.

Walch SG, Tinzoh LN, Zimmermann BF, Stühlinger W, Lachenmeier DW. 2011. Antioxidant capacity and polyphenolic composition as quality indicators for aqueous infusions of Salvia officinalis (sagetea). Frontiers Pharmacology 2(79), 1-6.

Wanke B, Lazéra MD, Nucci M. 2000. Fungal infections in the immune compromised host. Memórias do Instituto Oswaldo Cruz 95(1), 153-158.

Wojcikowski K, Johnson DW, Gobé G. 2004. Medicinal herbal extracts – renal friend or foe? Part one: the toxicities of medicinal herbs. Nephrology 9(5), 313-318.

World Health Organization (WHO). 1999. World Health Organization report on infectious diseases. Removing Obstacles to Healthy Development.

World Health Organization (WHO). 2000. Report on global surveillance of epidemic-prone infectious diseases. Geneva, Switzerland.

World Health Organization (WHO). 2002. Traditional Medicine Strategy. Geneva, Switzerland.

World Health Organization (WHO). 2014. Antimicrobial resistance. Global Report on Surveillance.

World Health Organization (WHO). 2016a. Infectious diseases fact sheet.

World Health Organization (WHO). 2016b. Antibiotic resistance fact sheet.

Xie Q, Johnson BR, Wenckus CS, Fayad MI, Wu CD. 2012. Efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro tooth model. Journal of Endodontics 38(8), 1114-1117.

Yineger H, Yewhalaw D. 2007. Traditional medicinal plant knowledge and use by local healers in Sekoru District, Jimma Zone, Southwestern Ethiopia. Journal of Ethnobiology and Ethno medicies 3(1), 1-7.

Yoneyama H, Katsumata R. 2006. Antibiotic resistance in bacteria and its future for novel antibiotic development. Biosciences, Biotechnology and Biochemistry 70(5), 1060-1075.

Zhang H, Qiu M, Chen Y, Chen J, Sun Y, Wang C, Fong HS. 2002. Plant terpenes. Phytochemistry and pharmacognosy. Encyclopedia of life support systems. http://www.eolss.net/Sample-Chapters/C06/E6-151-05-00.pdf.

Zwenger S, Basu C. 2008. Plant terpenoids: Applications and future potentials. Biotechnology and Molecular Biology Reviews 3(1), 1-7.