A study on synthesis of amylase, cellulase L-asparaginase enzymes and potassium solubulizing efficiency of rhizobacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India
Paper Details
A study on synthesis of amylase, cellulase L-asparaginase enzymes and potassium solubulizing efficiency of rhizobacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India
Abstract
The present study was aimed to screen the potassium solubilizing rhizobacteria from Betel vine plant (Piper betle) of Guntur district (Nutakki, Revendrapadu), Andhra Pradesh, India. In present study nutrient agar and Aleksandrov medium (A) were used for the isolation of rhizobacteria. A total of 15 rhizobial strains designated as ASN-1 to ASN-15 were isolated. All the strains were tested for their efficiency in synthesizing amylase, cellulase L-asparaginase enzymes. Apart from these all the strains are also tested for their Potassium solubulizing efficacy. Highly potent strain was based on its morphological, biochemical and molecular characteristics (16s r RNA sequencing). Of all the rhizobial strains, ASN-5 showed high potassium solubilization efficiency 15 mm at 25 μg/ml on Aleksandrov medium after 72 h of incubation at 28±2 ⁰C. Beside potassium solubilization strain ASN-5 is able to produce amylase, cellulase and L-asparaginase enzymes. The potential strain was characterized as Bacillus endophyticus ASN-5 (MW537708).
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M. Roja Susmitha, K. Ammani, 2024. A study on synthesis of amylase, cellulase L-asparaginase enzymes and potassium solubulizing efficiency of rhizobacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India. Int. J. Biosci., 24(6), 74-80.
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