A study on the effects of methyl jasmonate on morphological traits of Hypericum perforatum using leaf and stem explants

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Research Paper 01/07/2015
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A study on the effects of methyl jasmonate on morphological traits of Hypericum perforatum using leaf and stem explants

Amir Rajabi, Hussein Abbaspour, Jafar Masoud Sinaki, Saeid Gharib Bolouk
J. Bio. Env. Sci.7( 1), 526-536, July 2015.
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This study examines how methyl jasmonate (MeJA) as chemical elicitor on MS medium affects the morphological traits of a plant (i.e. number of leaves, stem length, number of roots, root length and number of buds) using different explants in vitro over a period of 42 days. The explants consisted of leaves and stems from seedlings cultured on MS medium under sterile conditions. They were transferred to the MS medium containing methyl jasmonate as elicitor at several concentrations including control, 50, 100, 150, 250 and 500 µmol, at temperature of 3±21 °C and 16:8 hours light/dark per day. This research was conducted as a factorial experiment based on Randomized Complete Block Design (RCBD) through 3 replications with 8 explants each. The data analysis at significant level of 1% suggested that methyl jasmonate as elicitor affects the entire morphological traits under study. The highest number of leaves, stem length and number of buds at 250 µmol of methyl jasmonate were on average 3.56, 1.38 and 1.53 cm, respectively. Furthermore, the highest mean of stem length (1.58 cm) and number of roots (1.15) were observed in the leaves explant at 500 µmol of methyl jasmonate. Therefore, it is concluded that methyl jasmonate on the traits affected and leaf explants were the best explants.


Balbi V, Devoto A. 2008. Jasmonate signalling network in Arabidopsis thaliana, crucial regulatory nodes and new physiological scenarios. New Phytol. National Center for Biotechnology Information. 177(2), 301 – 318.

Bairu M, Stirk W, Dolezal K, Staden JV. 2007. Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its

derivatives serve as replacement for benzyladenine and zeatin. Plant Cell Tiss. Organ Cult, 90, 15-23.

Bombardelli E, Morazzoni P. 1995. Hypericum Perforatum. Fitoterapia 66(4), 43-68. www.fortitechpremixes.com/…/bombardelli-e-morazzoni-p-vitis-vinifera.

Cao Sh, Zheng Y, Wang K, Rui H, Tang SH. 2009. Effect of methyl jasmonate on cell wall modification of loquat fruit in relation to chilling injury after harvest. Food Chemistry. 118, 641-647.

Choudhury S, Panda SK. 2004. Role of salicylic acid in regulating cadmium induced oxidative stress in Oryza Sativa L. roots. Bulg Journal of Plant Physiology 30, 95-110.

Clozas LM, Toro FJ, Calv G, Pelacho AM. 1999. Effect of Methyl Jasmonate on the first developmental stages of globe artichoke. International Society for Horticultural Science. Acta Horticulturae660 5th International Congress on Artichoke. Bari, Italy.

Crockett S. 2010. Essential Oil and volatile components of the genus Hypericum (Hypericaceae) Nat. Prod. Commun 5(9), 506-1493.

Deltito J, Bayer D. 1998. The scientific, quasi-scientific and popular literature on the use of St. John’s wort in the treatment of depression. Journal of Affective Disorders 51, 345-351.

Gao XP, Wang XF, Lu YF, Hang LY, Shen Y, Liang Z, Zhang DP. 2004. Jasmonjc acid is involved in the water-stressinduced betaine accumulation in pear leaves. Plant Cell and Environment 27(3), 447-507.

Ghanati F, Bakhtiarian S, Abdolmaleki P. 2010. Effect of MeJA on Secondary Metabolites of Marigold. Biological Science and Technology Journal Modarres. 1, 21-33.

Ghasemi Pirbaluti A, Eshrafi M, Rahim Malek M, Hamedi B. 2012. Effect of Foliar Jasmonate Acid on Percentage and Composition of Thymus Daenensis. Herbs. 2(2), 75-80.

Ghasemi A. 2009. “Understanding and Evaluating Aromatic Herbs and their Effects” Islamic Azad University, Shahr-e Jurd Branch, 474-476.

Keramat B, Daneshmand F. 2011. Dual Role of Methyl Jasmonate on the Physiological Functions of Soybean. Plant Process and Function. 1(1), 26-37.

Kosuth J, Koperdakova J, Tolonen A, Hohtola A, Cellarova E. 2003. The content of hypericins and phlorog lucinols in Hypericum perforatum L. seedlings at early stage of development. Plant Science. 165(4), 515-521.

Kozlowski G, Buchala A, M´etraux JP. 1999. Methyl jasmonate protects Norwayprotects Norwayprotects Norway spruce [Picea abies (L.) Karst.] seedlings against Pythium against Pythium ultimum Trow. Physiol. Mol. Plant Pathol 55(3), 53 – 58.

Pourshafee Anaraki F. 2000. Production and Maintenance of Nepeta Persica Bioss and Comparison of Metabolites in Production of Callus and Whole Plant” A PhD dissertation, general practitioner, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 42 pages.

Salimi F, Shekari F. 2012. Effect of MeJA and Salinity Stress on Some Morphological Traits and Functions of German chamomile (Matricaria chamomilla L.). Plant Biologym Spring issue. 4(11), 27-38.

Sembdner G, Parthier B. 1993. The biochemistry and the physiological molecular actions of jasmonates. Annu. Rev. Plant Physiol. Plant Mol 44, 569-589.

Rezaie M, Jaimand K, Noruzi H, Naderi M. 2001. A Study of Hypercin Compositions in Different Species of Hypericum Perforatum. Research and Development Journal. (2)14, 97-94.

Rossato L, Le Dantec C, Laine P, Ourry A. 2002. Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: identification, and immunolocalization of a putative taproot storage glycoprotein. J. Exp. Bot. 53, 265 – 275.

Masijuska B, Kopcewicz J. 2002. Inhibitory effect of methyl jasmonate on flowering and elongation growth in phatbitis nil. Journal of Plant Growth Regulation 21, 216-223.

Martin D, Tholl D, Gershenzon J, Bohlmann J. 2002. Methyl jasmonate induces traumatic resin ducts, terpenoid resin biosys-thesis, and terpenoid accumulation in developing xylem of Norway spruce stems. Plant Physiology 129, 1003-1018.

Zobayed SMA, Afreen F, Kozai T. 2005. Temperature stress can alter the photosynthetic efficiency and secondary metabolite concentrations in St. John’swort. Plant Physiol Biochem. 43, 977–984.