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Research Paper | July 1, 2015

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A study on the effects of methyl jasmonate on morphological traits of Hypericum perforatum using leaf and stem explants

Amir Rajabi, Hussein Abbaspour, Jafar Masoud Sinaki, Saeid Gharib Bolouk

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J. Bio. Env. Sci.7(1), 526-536, July 2015

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Abstract

This study examines how methyl jasmonate (MeJA) as chemical elicitor on MS medium affects the morphological traits of a plant (i.e. number of leaves, stem length, number of roots, root length and number of buds) using different explants in vitro over a period of 42 days. The explants consisted of leaves and stems from seedlings cultured on MS medium under sterile conditions. They were transferred to the MS medium containing methyl jasmonate as elicitor at several concentrations including control, 50, 100, 150, 250 and 500 µmol, at temperature of 3±21 °C and 16:8 hours light/dark per day. This research was conducted as a factorial experiment based on Randomized Complete Block Design (RCBD) through 3 replications with 8 explants each. The data analysis at significant level of 1% suggested that methyl jasmonate as elicitor affects the entire morphological traits under study. The highest number of leaves, stem length and number of buds at 250 µmol of methyl jasmonate were on average 3.56, 1.38 and 1.53 cm, respectively. Furthermore, the highest mean of stem length (1.58 cm) and number of roots (1.15) were observed in the leaves explant at 500 µmol of methyl jasmonate. Therefore, it is concluded that methyl jasmonate on the traits affected and leaf explants were the best explants.

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A study on the effects of methyl jasmonate on morphological traits of Hypericum perforatum using leaf and stem explants

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