Accumulation of heavy metals and feeding habits in Cyprinidae fishes of water bodies of Khuzdar, Balochistan, Pakistan

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Research Paper 01/03/2020
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Accumulation of heavy metals and feeding habits in Cyprinidae fishes of water bodies of Khuzdar, Balochistan, Pakistan

Lawang Gurganari, Ghulam Dastagir, Rahana Mushtaq
Int. J. Biosci.16( 3), 144-156, March 2020.
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The research area is at Khuzdar district, we evaluate heavy metals concentration in water bodies and fish organs cyprinid fish (Cyprinus carpio,Cirinus mirigla,Tor putitora, Labeorohuta and Catlacatla). We discuss the water quality, physio-chemical changes and nutritional behavior of fishes. The heavy metals i.e. Fe, Cd, Pb, and Cr were identified by Atomic Absorption Spectroscopy (AAS) method. Metal concentration similar as well varied over four stations such, Zedi, Wangoo, Mola Chtook, and Wadh Poralli. Metals absorption followed the order: Fe > Cr >Pb> Cd. Gills and liver showed comparatively highest heavy metals concentration. Consequently, all cyprinid fishes presented statically important variations in all organs. Gut analysis of C.mirigla, gut examination by preponderance grade by volume of food content green algae, detritus, earth worm, mud, miscellaneous, phytoplankton, and zooplankton. L. rohita however, showed grade green algae, detritus, mud, earth worm, miscellaneous, zooplankton, and phytoplankton. C. carpiogreen algae, mud, detritus, miscellaneous, earth worm, phytoplankton, and zooplankton. T. putitora earth worm, green algae, mud, detritus, miscellaneous, phytoplankton, zooplankton, however in C. catla miscellaneous, mud, green algae, earth worm, detritus, phytoplankton, and zooplankton. The Fe concentration highest in all kinds of fishes, Cr showed Pb second highest accumulation two kinds, while Cd least in all fishes. Only Fe which is above the prescribed limit of WHO that can pose severe health risk to population of nation.


Alp A, Yeğen V, ApaydinYağci M, Uysal R. Biçen E, Yağci A. 2008. Diet composition and preyselection of the pike, Esoxlucius, in Civril Lake, Turkey. Journal of Applied Ichthyology 24(6), p 670-677.

Andrews NC, Baltimore D. 1986. Purification of a terminal uridylyl transferase that acts as host factor in the in vitro poliovirus replicase reaction. Proceedings of the National Academy of Sciences 83(2), p 221-225.

Andrews NC. 1999. Disorders of iron metabolism. New England Journal of Medicine, 341(26), p 1986-1995.

Araoye PA. 2009. The seasonal variation of pH and dissolved oxygen (DO2) concentration in Asa lake Ilorin, Nigeria. International Journal of Physical Sciences 4(5), p 271-274.

Balochistan GG. 2018. Published by the authority Government of Balochistan, Quetta Wednesday    June 27, p 249.

Bakhtiyar Y, Langer S, Karlopia SK, Chalotra RK. 2017. Studies on the feeding habits of Labeorohita (Ham.) from Gho-Manhasa fish ponds, Jammu, North India. Journal of Ecophysiology and Occupational Health 17(1-2), 40-49.

Chughtai  MI, Mahmood K. 2012. Semi-intensive carp culture in saline water-logged area: A multi-location study in Shorkot (District Jhang), Pakistan. Pakistan Journal of Zoology 44(4), 1065-1072.

Dallman PR. 1986. Biochemical basis for the manifestations of iron deficiency. Annual review of nutrition 6(1), p 13-40.

Dewan S, Miah JU, Sarker AL, Saha SN. 1979. Seasonal patterns of feeding of juvenile major carp, Labeorohita (Ham.) in a Bangladesh pond. Journal of Fish Biology 14(6), 511-515.

Dulic Z, Subakov-Simic G, Ciric M, Relic R, Lakic N, Stankovic M, Markovic Z. 2010. Water quality in semi-intensive carp production system using three different feeds. Bulgaria Journal of Agriculture Science 16(3), p 266-274.

Effendy I, Al Deen S, Chithambaran S. 2016. Semi Intensive and Semi Biofloc Methods for the Culture of Indian White Prawn, Fenneropenaeus indicus in High-density Polyethylene Liner Ponds. HAYATI Journal of Biosciences 23(3), p 106-110.

El Moghraby AI, el Rahman AA. 1984. Food and feeding habits of Labeoniloticus (Pisces, Cyprinidae) in Jebel Aulia Reservoir, Sudan. In Limnology and Marine Biology in the Sudan Springer, Dordrecht p 327-332.

Eschmeyer WN, Fong JD. 2015. Species by family/subfamily in the Catalog of Fishes. San Francisco, CA: California Academy of Sciences.

Förstner U, Wittmann GTW. 1981. Heavy metals in the aquatic environment.

Gupta T, Dey M. 2013. Hydro biological characteristics of some semi-intensive fish culture ponds of Lumding town of Nagaon district, Assam. Current World Environment 8(1), p.107.

Guruge WAHP, Amarasinghe NJ, De S. 2008. Reported the food resource utilization of  three  co-occuring  carnivorous  fish  species  sin  shallow  lowland reservoir  in  South  Eastern  Sri  Lanka:  Approach  to  minimize  competition. Proceeding of Taala 2007: The 12th World Lake Conference: 141-148.

Has-Schön E, Bogut I, Kralik G, Bogut S, Horvatić J, Čačić I. 2008. Heavy metal concentration in fish tissues inhabiting waters of “BuškoBlato” reservoar (Bosnia and Herzegovina). Environmental monitoring and assessment 144(1-3), p 15-22.

Heath AG. 1987. Behavior and nervous system function. Water pollution and fish physiology, p 181-196.

Hora SL, Pillay TV R. 1962. Hand book of fish culture in the Indo-Pacific region, Food and Agriculture Organization of the United Nations. Biology Branch, Fisheries Division p 204.

Hynes HBN. 1950. The food of fresh-water sticklebacks (Gasterosteus aculeatus and Pygosteuspungitius), with a review of methods used in studies of the food of fishes. The Journal of Animal Ecology, p 36-58.

Iqbal A, Tabinda AB, Ahmad F, Yasar A, Siddique S. 2016. Temporal Metal Bioaccumulation in Tissues of Labeorohita and Cyprinus carpio from Indus River, Pakistan. Asian Journal of Chemistry 28(5), p.1069.

Islam ML, Alam MJ, Rheman S, Ahmed SU. Mazid MA. 2004. Water quality, nutrient dynamics and sediment profile in shrimp farms of the Sundarbans mangrove forest, Bangladesh.Indian journal of Marine Sciences 33(2), 170-176.

Jabeen F, Chaudhry AS. 2010. Environmental impacts of anthropogenic activities on the mineral uptake in Oreochromis mossambicus from Indus River in Pakistan. Environmental monitoring and assessment 166(1-4), p641-651.

Kargin F. 1996. Seasonal changes in levels of heavy metals in tissues of Mullus barbatus and Sparus aurata collected from Iskenderun Gulf (Turkey). Water, Air, and Soil Pollution 90(3-4), p 557-562.

Kassahun AD. 2005. Distribution, abundance and feeding biology of fish species in Koka reservoir and the associated Awash River flood plain, Ethiopia. (unpublished Thesis Master of Science, UNESCO-IHE Institute for Water Education), p 123.

Khan RA, Siddiqui AQ. 1973. Food selection by Labeorohita (Ham.) and its feeding relationship with other major carps. Hydrobiologia, 43(3-4), 429-442.

Kishor B, Bhatt JP, Rawat VS, Nautiyal P, 1998. Variations in food habit of the Himalayan mahseer—Tor putitora (Ham.) inhabiting the Ganga river system in Garhwal region. Indian J Fish, 45(1), p 113-118.

Langston WJ. 1990. Toxic effects of metals and the incidence of metal pollution in marine ecosystems. Heavy metals in the marine environment, p 101-122.

Lashari KH, Korai AL, Sahato GA, Kazi TG. (2009). Limnological studies of keenjhar lake, district, Thatta, Sindh, Pakistan. Pakistan Journal of Analytical and Environmental Chemistry, 10(1-2), p 39-47.

Lenntech H. 2004. Lenntech Water Treatment and Air Purification. Water Treatment. Rotterdamseweg, Netherlands, p l0.

Malik Y, Achakzai WM, Muhammad S, Mustafa S, Saddozai S, Qadir A, Ahmad N. and Baloch IA. 2017. Determination of heavy metal concentrations in six species of fresh water fish sold in Quetta city, Pakistan. Pure and Applied Biology 6(1), p 79.

Miah MJU, Dewan S, Wahiduzzaman M. 1984. Studies on the type and amount of food taken by the silver carp, Hypophthalmichthys molitrix (val) and its diel patterns of feeding in a Bangladesh pond. Bangladesh Journal of Fisheries (Bangladesh).

Mishra MK, Mishra N, Pandey DN. 2013. An assessment of the physicochemical characteristics of Bhamka pond, Hanumana, Rewa district, India. International Journal of Innovative Research in Science, Engineering and Technology 2(5), p 1781-1788.

Mishra RR, Rath B, Thatoi H. 2008. Water quality assessment of aquaculture ponds located in Bhitarkanika mangrove ecosystem, Orissa, India. Turkish Journal of Fisheries and Aquatic Sciences 8(1), p 71-77.

Nazish N, Mateen A. 2011. Winter growth of carps under different semi-intensive culture conditions. Pakistan Veterinary Journal 31(2), p 134-136.

Noor K, Naureen AQ, Nasir M, Fayyaz R, Khalid JI. 2011.  Effect of artificial diet and culture systems on sensory quality of fried Fish flesh of Indian major carps. Pakistan journal of zoology, 43(6), p 1177-1182.

Nriagu JO, Pacyna JM. 1988. Quantitative assessment of worldwide contamination of air, water and soils by trace metals. Nature 333(6169), p 134.

Obasohan EE. 2007. Heavy metals concentrations in the offal, gill, muscle and liver of a freshwater mudfish (Parachanna obscura) from Ogba River, Benin city, Nigeria. African Journal of Biotechnology 6(22).

Obasohan EE, Oronsaye JAO, Obano EE, 2006. Heavy metal concentrations in Malapteruruselectricus and Chrysichthysnigrodigitatus from Ogba River in Benin City, Nigeria. African Journal of Biotechnology 5(10),

Oguzie FA. 2003. Heavy metals in water and sediment of the lower Ikpoba River, Benin City, Nigeria. Pakistan Journal of Scientific and Industrial Research (Pakistan).

Oze G, Oze R, Anunuso C, Ogukwe C, Nwanjo H, Okorie K. 2006. Heavy metal pollution of fish of Qua-Iboe river estuary: Possible implications for neurotoxicity. International Journal of Toxicology 3, p 56-59.

Pillay TVR. 1952. A Critique of the methods of study of food of fishes. Journal of Zoology Society.

Saikia SK, Das DN. 2009. Feeding ecology of common carp (Cyprinus carpio L.) in a rice–fish culture system of the Apatani plateau (Arunachal Pradesh, India). Aquatic Ecology 43(2), 559-568.

Saikia SK, Das DN. 2009. Feeding ecology of common carp (Cyprinus carpio L.) in a rice–fish culture system of the Apatani plateau (Arunachal Pradesh, India). Aquatic Ecology 43(2), 559-568.

Sangpradub N, Boonsoong B, Mekong River Commission Vientiane (Lao PDR). 2006. Identification of freshwater invertebrates of the Mekong River and its tributaries. Mekong River Commission- Freshwater invertebrates – p 274.

Shi PS, Wang Q, Zhu YT, Gu QH, Xiong BX, 2013. Comparative study on muscle nutritional composition of juvenile bighead carp (Aristichthysnobilis) and paddlefish (Polyodonspathula) fed live feed. Turkish Journal of Zoology 37(3), p 321-328.

Sipaúba-Tavares LH, Millan RN, Milstein A. 2016. Limnology of an integrated cage-pond aquaculture farm. Acta Limnologica Brasiliensia, 28.

Steel RGD, Torrie JH, Dickey DA. 1996. Principles and procedures of statistics A biometrical approach 3rd ed McGraw Hill Book Company Inc. New York, USA p, 334-381.

Talwar PK, Jhingran AG. 1991. Inland fisheries of India and adjacent countries.  1(2), p1-1158.

Talwar PK. 1991. Inland fishes of India and adjacent countries (2), CRC Press.

Tsui MT, Wang WX.  2004. Uptake and elimination routes of inorganic mercury and methylmercury in Daphnia magna. Environmental science & technology 38(3), p 808-816.

Wahab MA. 1994. Compatibility of silver carp in the polyculture of cyprinid fishes. Progress Agric, 5, 221-227.

WALI MA. 2014. Post super flood studies on vertebrate biodiversity and production in manchar lake, District Jamshoro.

World Health Organization. 2003. Nitrate and nitrite in drinking-water: Background document for development of WHO Guidelines for Drinking-water Quality (No. WHO/SDE/WSH/04.03/56). World Health Organization.

Yilmaz AB. 2003. Levels of heavy metals (Fe, Cu, Ni, Cr, Pb, and Zn) in tissue of Mugil cephalus and Trachurusmediterraneus from Iskenderun Bay, Turkey. Environmental research, 92(3), p 277-281.

Zhang H. 2006. Leaching behavior of Pb and Zn in air pollution control residues and their modeling prediction. Journal of Environmental Sciences, 18(3), p 583-586.

Zheng LP, Yang JX, Chen XY, Wang WY. 2010. Phylogenetic relationships of the Chinese Labeoninae (Teleostei, Cypriniformes) derived from two nuclear and three mitochondrial genes. Zoologica Scripta 39(6), p 559-571.