Acute and subacute toxicity of the aqueous extract of the leaves of Annona senegalensis Pers. (Annonacee) in rats and mice
Paper Details
Acute and subacute toxicity of the aqueous extract of the leaves of Annona senegalensis Pers. (Annonacee) in rats and mice
Abstract
Annona senegalensis pers. is used in Africa for therapeutic and dietary purposes. However, few studies have been carried out in Burkina Faso on the toxicity of this plant’s leaves. The aim of the study was to assess the acute and subacute toxicity of the aqueous extract of the leaves of Annona senegalensis on female rats and mice. The acute toxicity study was carried out on six female mice. The control group, received distilled water and the the test group received the extract at a single dose of 2000 mg/ kg bw. After administration, animals were observed for 14 days. For subacute toxicity, thirty female rats were used. The control group received distilled water. The test groups received Annona senegalensis extract at doses of 40, 100 and 200 mg/kg bw respectively for 28 consecutive days. Satellite groups were used. In the acute test, no sign of toxicity or mortality were recorded. The LD50 of the extract is therefore greater than 2000 mg/kg. In the sub-acute test, the body weight of treated rats increased (p> 0.05) compared to the control. Hematological analyses of white and red blood cell and platelet counts showed no significant variations (p> 0.05) compared with the control. Biochemical analyses showed no significant variations (p> 0.05) compared with the control. Histological sections showed no tissue damage in the liver and kidneys of rats treated with extract compared with the control. Our results show that the leaves of Annona senegalensis were non-toxic.
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