Acute toxicity of seeds from Sphenostylis stenocarpa (Hochst ex A. Rich.) Harms

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Research Paper 11/08/2026
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Acute toxicity of seeds from Sphenostylis stenocarpa (Hochst ex A. Rich.) Harms

Wilfried Zanmenou*, Razack Osseni, Mahouglo Barnabé Houessou, Chimène Agrippine Rodogune Yelouassi, Comlan Kintomagnimessè Célestin Tchekessi, Philippe Sessou, Anatole Laleye, Pierre Dossou-Yovo
Int. J. Biosci. 29(2), 89-96, August 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

The major fear bound to the consumption of leguminous plant is relative to the concentration of secondary metabolites accumulated in reserve organs such as seeds. These metabolites can induce an anti-nutritional or toxic effect to various degrees according to their nature and concentration. The present work aims to achieve a toxicity study of the seeds of Sphenostylis stenocarpa commonly called African Yam Bean. At the end of the phytochemical screening made on the flours of the seeds in the state and roasted, aiming to put in evidence the alkaloids, coumarins, tannins, flavonoids and saponosides, only the tannins, flavonoids and saponosides proved to be positive. The same analysis revealed the absence of these metabolites in boiled seed flour and the presence of tannins and flavonoids in the cooking water. Observation of the impact of the content of these present metabolites in the seeds on the biological and histological parameters of the experimental rats as compared to the control rats do not reveal any significant differences or particular remarks. It emerges from this study that Sphenostylis stenocarpa does not have a toxic effect on the health of the consumer, who should fully benefit from its nutritional potential.

Amoatey HM, Klu GYP, Bansa D, Kumaga FK, Aboagye LM, Benett SO, Gamedoagbao DK. 2000. African yam bean (Sphenostylis stenocarpa): A neglected crop in Ghana. West African Journal of Applied Ecology 1, 53–60.

Apata DF, Ologhobo AD. 1990. Some aspects of biochemistry and nutritive value of African yam bean seed (Sphenostylis stenocarpa). Food Chemistry 36, 271–280.

Azeke MA, Fretzdorff B, Buening-Pfaue H, Betsche T. 2007. Comparative effect of boiling and solid substrate fermentation using the tempeh fungus (Rhizopus oligosporus) on the flatulence potential of African yam bean (Sphenostylis stenocarpa) seeds. Food Chemistry 103, 1420–1425.

Dansi A, Vodouhè R, Azokpota P, Yedomonhan H, Assogba P, Adjatin A, Loko YL, Dossou-Aminon I, Akpagana K. 2012. Diversity of the neglected and underutilized crop species of importance in Benin. The Scientific World Journal 2012, 1–19.

Duodu KG, Taylor JRN, Belton PS. 2003. Factors affecting sorghum protein digestibility. Journal of Cereal Science 38, 117–131.

Edem DO, Amuga CI, Eka OU. 1990. Chemical composition of African yam beans. Tropical Science 30, 59–63.

Ekanayake S, Jansz ER, Nair BM. 2000. Literature review of an underutilized legume: Canavalia gladiata L. Plant Foods and Human Nutrition 55, 305–321.

Ezeonwumelu JO, Julius AK, Muhoho CN, Ajayi AM, Oyewale AA, Tanayen JK, Balogun SO, Ibrahim A, Adzu B, Adiukwu CP, Oloro J. 2011. Biochemical and histological studies of aqueous extract of Bidens pilosa leaves from Ugandan Rift Valley in rats. British Journal of Pharmacology and Toxicology 2(6), 302–309.

Fasayiro SB, Ajibadé SR, Omole AJ, Adeniyan ON, Farinde EO. 2006. Proximate, minerals and anti-nutritional factors of some underutilized grain legumes in south-western Nigeria. Nutrition and Food Science 36(1), 18–23.

Ghaniyah OA, Abiodun AO. 2016. Physical, proximate and anti-nutritional composition of African yam bean (Sphenostylis stenocarpa) seeds varieties. Journal of Food Research 5(2), 67–72.

Gilani GS, Cockell KA, Sepehr E. 2005. Effects of antinutritional factors on protein digestibility and amino acid availability in foods. Journal of AOAC International 88, 967–987.

Ndidi US, Ndidi CU, Abbas O, Aliyu M, Francis GB, Oche O. 2014. Proximate, antinutrients and mineral composition of raw and processed (boiled and roasted) Sphenostylis stenocarpa seeds from Southern Kaduna, Northwest Nigeria. 2014, Article ID 280837, 9 pages.

Nehad NAM. 1990. Effect of presoaking on faba bean enzyme inhibitors and polyphenol after cooking. Journal of Agricultural and Food Chemistry 38, 1479–1482.

Nyananyo BL, Nyingifa AL. 2011. Phytochemical investigation on the seed of Sphenostylis stenocarpa (Hochst ex A. Rich.) Harms (Family Fabaceae). Journal of Applied Sciences and Environmental Management 15(3), 419–423.

Olson H, Betton G, Robinson D, Thomas K, Monro A, Kolaja G, Lilly P, Sanders J, Sipes G, Bracken W, Dorato M, Deun KV, Smith P, Berger B, Heller A. 2000. Concordance of toxicity of pharmaceuticals in humans and in animals. Regulatory Toxicology and Pharmacology 32, 56–67.

Onuoha CH, Harry BJ, Eze SO. 2017. Evaluation of nutrients and anti-nutritional factors of different species of African yam bean (Sphenostylis stenocarpa). European Journal of Basic and Applied Sciences 4(1), 1–8.

Teo S, Stirling D, Thomas S, Hoberman A, Kiorpes A, Khetani V. 2002. A 90-day oral gavage toxicity study of d-ethylphenidate and dimethylphenidate in Sprague–Dawley rats. Toxicology 174, 183–196.

Wagner H, Bladt S. 2001. Plant Drug Analysis. 2nd ed. Springer, Berlin, Heidelberg, New York.

Yakubu MT, Akanji MA, Oladiji AT. 2007. Haematological evaluation in male albino rats following chronic administration of aqueous extract of Fadogia agrestis stem. Pharmacognosy Magazine 3, 34–38.

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