Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba

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Research Paper 01/04/2017
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Adaptation and optimization of various physico-chemical conditions for pyrene degradation by bacteria isolated from the Rhizospheric soil of Morus alba

Nadia Bibi, Mohammad Hamayun, Anwar Hussain, Aaqib Sayyed, Sumera Afzal Khan
Int. J. Biosci.10( 4), 343-353, April 2017.
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Isolation from contaminated sites using classical enrichment technique is of common practice and give promising results but with a narrow range of microbial community. In this study, wild bacteria were isolated and adopted through changing growth media and exposure to UV-light for 15minutes. Various physico-chemical conditions were optimized for pyrene degradation by isolate M3 with 1000ppm concentration, optimum temperature of 35°C, pH 7 and shaking speed of 120 rpm. Isolate M3 degraded 76.31% pyrene with 0.970 OD value and 2.7×10-31 CFUmL-1 after 120 hours. Metabolites formed during degradation process on comparison with authentic standards were identified as phenanthrene, catechol, salicylaldehyde and salicylic acid. Plasmid isolation and curing showed a fractional reduction in pyrene degradation by this isolate. Experiment was performed in triplicate and data was analysed by 2-way ANOVA and level of significance 0.002.


Abdelhay A, Magnin JP, Gondrexon N, Baup S, Willison. 2008. Journal of Applied Microbiology and Biotechnology 78, 881888.

Ahmed S, Ahmed S, Nisar MF, Hussain K, Majeed A, Ghumroo PB, Afghan S, Shahzad A, Nawaz K, Ali K. 2010. Isolation and characterization of a bacterial strain for aniline degradation. African Journal of Biotechnology 9, 11731179.

Ahmed S, Ahmed S, Nisar MF, Hussain K, Majeed A, Ghumroo PB, Afghan S, Shahzad A, Nawaz K, Ali K. 2010. Isolation and characterization of a bacterial strain for aniline degradation. African Journal of Biotechnology 9, 11731179.

Alias S, Abdul-Talib S, Omar M, Hussain NH. 2011. “Degradation of 3-ring PAHs by S. spiritovorum and C. urealyticum Strain under Non Indigenous Condition,” in International Congress of Chemistry and Environment (ICCE 2011), Port Dockson Malaysia.

Arulazhagan P, Vasudevan N. 2014. Biodegradation of polycyclic aromatic hydrocarbons by a halotolerant bacterial strain Ochrobactrum sp. VA1. Marine Pollution Bulletin 62, 388394.

Deeb RA, Alvarez-Cohen L. 1999. Temperature Effects and Substrate Interactions during the Aerobic Biotransformation of BTEX Mixtures by Toluene-Enriched Consortia and Rhodococcus rhodochrous; Biotechnology and Bioengneering 62, 526536.

Doick KJ, Dew NM, Semple KT. 2005. Linking catabolism to cyclodextrin extractability: determination of the microbial availability of PAHs in soil. Environmental Science and Technology 39, 88588864.

Ebrahimi M, Sarikhani MR, Fallah R. 2012. Assessment of biodegradation efficiency of some isolated bacteria from oil contaminated sites in solid and liquid media containing oil-compounds. International Research Journal of Applied and Basic Sciences 3, 138147.

Gaskin S, Bentham R. 2005. Comparison of enrichment methods for the isolation of pyrene-degrading bacteria. International Biodeterioration and Biodegradation 56, 8085.

Guo C, Dang Z, Wong Y, Tam N. 2010. Biodegradation ability and dioxgenase genes of PAH-degrading Sphingomonas and Mycobacterium strains isolated from mangrove sediments. International Journal of Biodeterioration and Biodegradation. 64, 419426.

Guo CL, Zhou HW, Wong YS, Tam NFY. 2005. Isolation of PAH-degrading bacteria from mangrove sediments and their biodegradation potential. Marine Pollution Bulletin 51, 10541061.

Haritash AK, Kaushik CP. 2009. Review on Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs). Journal of Hazardous Material 169, 1-15.

Johnsen AR, Wick LY, Harms, H. 2005. Principles of microbial PAH-degradation in soil. Environmental Pollution. 133, 71-84.

Juhasz AL, Britz ML, Stanley GA. 1997. Degradation of fluoranthene, pyrene, benz [a] anthracene and dibenz [a,h] anthracene by Burkholderia cepacia. Journal of. Applied Microbiology 83, 189-198.

Kafilzadeh F, Sahragard P, Jamali H, Tahery Y. 2011. Isolation and identification of hydrocarbons degrading bacteria in soil around shiraz refinery. African Journal of Microbiology Research 4, 3084-3089.

Kastner M, Breuer JM, Mahro B. 1998. Impact of inoculation protocols, salinity and pH on degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of PAH-degrading bacteria introduced into soil. Applied and Environmental Microbiology 64, 359-62.

Khan JA, Sha R. 2011. Isolation and characterization of micro-organism from oil contaminated sites. Advances in Applied Science Research 2, 455-460.

Khan K, Naeem M, Arshed MJ, Asif M. 2006. Extraction and characterization of oil degrading bacteria. Journal of Applied Sciences 6, 2302-2306.

Kim SJ, Kweon O, Jones RC, Freeman JP, Edmondson RD, Cerniglia CE. 2007. Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology. Journal of Bacteriology 189, 464-472.

Kiyohara H, Nagao K, Yana K. 1982. Rapid Screen for Bacteria Degrading Water-Insoluble, Solid Hydrocarbons on Agar Plates. Applied and Environmental Microbiology 43, 454-457.

Krivobok S, Kuony S, Meyer C, Louwagie M, Willison JC, Jouanneau Y. 2003. Identification of pyrene-induced proteins in Mycobacterium sp. strain 6PY1: evidence for two ring-hydroxylating dioxygenases. Journal of Bacteriology 185,3828-3841.

Kumar G, Singla R, Kumar R. 2010. Plasmid Associated Anthracene Degradation by Pseudomonas sp. Isolated from Filling Station Site. Nature and Science 8(4), 89-94.

Lakshmi MB, Velan M. International 2nd Conference on Environmental Science and Technology IPCBEE. 2011, vol 6.

Leahy JG, Colwell RR. 1990. Microbial degradation of hydrocarbons in the environment. American Society of Microbiology 54(3), 305-315.

Li XJ, Li PJ, Lin X, Zhang CG, Li Q, Gong ZQ. 2008. Biodegradation of aged polycyclic aromatic hydrocarbons (PAHs) by microbial consortia in soil and slurry phases. Journal of Hazardous Material 150, 21-26.

Macleod CJA, Semple KT. 2006. The influence of single and multiple applications of pyrene on the evolution of pyrene catabolism in soil. Environmental Pollution 139, 455-460.

Miller CD, Child R, Hughes JE, Benscai M, Dar JP, Sims RC, Anderson AJ. 2007. Diversity of soil mycobacterium isolates from three sites that degrade polycyclic aromatic hydrocarbons. Journal of Applied Microbiology 102, 1612-1624.

Moscoso F, Deive FJ,  Longo MA, Sanroman MA. 2015. Insights into polyaromatic hydrocarbon biodegradation by Pseudomonas stutzeri CECT 930: operation at bioreactor scale and metabolic pathways. International Journal of Environmental Science and Technology 12, 1243-1252.

Mukesh KDJ, Sindhu G, Prasanna KP, Balakumaran MD, Kalaichelvan PT. 2012. Effect of Triton X-100 on Degradation of Polycyclic Aromatic Hydrocarbons by Pseudomonas sp. PSS6 Isolated from Municipal Wastes Sediment. Advances in Applied Science Research 3, 1383-1388.

Naveenkumar S, Manoharan N, Ganesan S, Manivannan SP, Velsamy G. 2010. Isolation, Screening and In Vitro Mutational Assessment of Indigenous Soil Bacteria for Enhanced Capability in Petroleum Degradation. International Journal of Environmental Sciences 2, 80-95.

Neelofur M, Prasad SV, Mahesh M. 2014. Enhance the Biodegradation of Anthracene by Mutation from Bacillus species. BMR Biotechnology 140, 1-19.

Nnamchi CI, Obeta JAN, Ezeogu LI. 2006. Isolation and characterization of some polycyclic aromatic hydrocarbon degrading bacteria from Nsukka soils in Nigeria. International Journal of Environmental Science and Technology 3, 181-190.

Nour Sh El, Gendy, Yasser M, Moustafa Salem A, Habib Sherif Ali. 2010. Evaluation of Corynebacterium variabilis Sh42 as a degrader for different poly aromatic compounds. Journal of American Science 6, 11.

Othman N, Hussain N, Talib SA. 2010. Degradation of polycyclic aromatic hydrocarbon by pure strain isolated from municipal sludge: Synergistic and cometabolism phenomenon. (ICENV) p. 86-90.

Pan SW, Wei SQ, Yuan X. 2008. “The Removal and Remediation of Phenanthrene and Pyrene in Soil by Mixed Cropping of Alfalfa and Rape,” Agricultural Science. in China 7, 1355-1364.

Park KS, Sims RC, Dupont R. 1990. Transformation of PAHs in soil systems. Journal of Environmental Engineering (ASCE) 116, 623-640.

Ping L, Zhang C,  Zhang C, Zhu Y,  He H, Wu M,  Tang T, Li Z,  Zhao H. 2014. Isolation and characterization of pyrene and benzo[a]pyrene-degrading Klebsiella pneumonia PL1 and its potential use in bioremediation. Applied Microbiology and Biotechnology 98, 3819-3828.

Puglisi E, Cappa F, Fragolis G, Trevisan A, Delre AAM. 2007. Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere 67, 548-556.

Rosmarie AF. 1993. Toxicity summary for fluoranthene.

Sartoros C, Yerushalmi L, Beron P, Guiot SR. 2005. Effects of surfactant and temperature on biotransformation kinetics of anthracene and pyrene. Chemosphere 61, 1042-1050.

Shokrollahzadeh S, Golmohammad F, Shokouhi H. 2012. Study of Sphingopyxis Isolates in Degradation of Polycyclic Aromatic Hydrocarbons. Chemical Engineering Transaction 27, 55-60.

Survery S, Ahmad S, Subhan SA, Ajaz M, Rasool SA. (2009). Hydrocarbon degrading bacteria from Pakistani soil: Isolation, identification, screening and genetical studies. Pakistan Journal of Botanical Sciences 7, 1518-1522.

Top EM, Springael D. 2003. The role of mobile genetic elements in bacterial adaptation to xenobiotic organic compounds. Current Opinion in Biotechnology 14, 262-269.

Vinas M, Sabate J, Espuny MJ, Solanas AM. 2005. Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil 71, 7008-7018.

Wick AF, Nicholas WH, Sukkariyah BF, Haering KC, Daniels WL. 2007. Remediation of PAH-Contaminated Soils and Sediments: A Literature Review Journal of Biotechnology 6, 107-113.